Takara Clark

**** ******** ** *-* Winterville, NC 28590

Phone: 336-***-****

E-Mail: aboab5@r.postjobfree.com

Objective

To utilize my real world experiences along my Bachelor's degree in Biology

to obtain a job working in a pharmaceutical lab so that I may help others

live healthier lives.

Education

December 2008

Bachelors of Science, Biology

East Carolina University, Greenville, NC

Classes included: General Biology, Microbiology, Genetics, Anatomy and

Physiology, Ecology, Evolutionary Biology, Marine Biology, Immunology,

training in research skill, Organic Chemistry, General Chemistry, Physics,

Biostatistics, Calculus, Introduction to Computers and Technical Writing

Minor Speech and Hearing Sciences

Training in phonetics, habilitation and rehabilitation in speech disorders,

skills with

hearing screenings, and some training in sign language

January 2011

Courses taken

Biochemistry and Protein Purification

Experience

July 2011 - Present

Hospira

. Aseptic Certified

. Biological Quality Certified

. Able to Process and Test: Waters for Injection, Purified Waters, and

Sterile Waters

. Have used of a Climet machine for non-viable air sampling

. Have collected viable air samples

. Able to sample areas for cleanliness via anaerobic, yeast and mold, and

trypticase soy agar

. Obtained knowledge and use of the Laboratory Information Management

Systems (LIMS)

. Have tested bacterial, yeast and mold growth

. Have maintained laboratory area

August 2005-May 2011

East Carolina University, Greenville, NC

Skills Acquired

. Able to make up reagents

. Calculate concentrations

. Have made up, poured, and loaded SDS-PAGE gels

. Western Blotting

. HPLC, Ion-exchange Chromatography, Size exclusion Chromatography

. Breaking open cells via French Press

. Able to concentrate materials using a concentrator

. Able to efficiently document and communicate my results with others

. Able to operate UV spectrophotometer

. Able to run protein and enzymatic assays

. Gram Staining, Sterilization techniques, Culturing techniques, Aseptic

techniques, and Serial dilutions

. Identifying and dissection of organs

. Tested water for pH, ppm, temperature, clarity, and nutrients.

. Have set up experiments to test the intraspecific competition of Betta

fish.

. Have set up experiments for interspecific competition of plants

. Analyzed data through observation and biostatistical methods

. Thin layer chromatography

. Extractions of organic compounds

. Analyzing data by UV spectroscopy

May 2008-December 2008

Research Assistant

Have been involved with a research team with a project aimed at developing

molecular markers for the study of the Japanese medaka embryonic

development.

Skills mastered:

. Preparing cDNA from an mRNA template

. Polymerase Chain Reaction (PCR)

. Endonuclease Digesting of DNA with restriction enzyme

. Plasmid construction through ligation of DNA fragment into plasmid

vectors

. Preparing, running, and photographing Agarose gels aimed at analyzing the

results of various molecular reactions

. Conducting Dot Blot reactions for quantitation of labeled riboprobes

. Microdissection of Japanese medaka embryonic head skeleton

. Use of an autoclave machine

Achievements

. Acquired Certified Nursing Assistant (CNA) certification through Medical

Careers program

. Presidential List (Spring 2005)

. Dean's List (Fall 2004)

. Honor Roll (Fall 2005, Spring 2006, Fall 2007)

. Voted Secretary of my dormitory

. Awarded $1000 scholarship

Employment History

July 2011 - Present

Hospira Pharmaceuticals, Rocky Mount,NC

Biological Quality Lab Analyst II

Using aseptic techniques, I sample: areas, waters, and products for

contamination.

March 2009 - January 2009; January 2011 - July 2011

East Carolina University Student Transit Authority, Greenville, NC

Student driver

Transportation of East Carolina University students.

May 2009 - January 2011

The Rush Fitness Complex, Greensboro, NC

Personal Trainer

To promote health and wellness, create exercise programs for my clients,

make sure the exercises are executed safely, help the members at the Rush,

and straighten up the used equipment of the members or other personal

trainers.

May 2004 - August 2006

Clapp's Nursing Center, Pleasant Garden, NC

Certified Nursing Assistant

In charge of patient care: providing a good quality of life for patients

and their families.

Keyword Summary

Biologist with Quantitative Communication Research Skill and CNA

Contact this candidate