Medical Development

Cover-letter

Date: March, ****

To Whom It May Concern:

I am looking for a scientific position in your company. I

think my professional background would match perfectly for

a position in your company. I have worked over 5 years as a

Scientist, R&D Biochemistry, Siemens. The primary focus of

my work was to characterize molecular allergenic proteins

(i.e small proteins) for diagnosis in Immunoassays. I have

researched on protein and related fields i.e. proteins,

antibodies, antigens and molecular proteins (allergens)

from different Institutes in post doctoral fellowship i.e.

Univ. of Arkansas for Medical Sciences (UAMS, AR), Mayo

Clinic (Rochester, MN), Harvard Medical School (Boston, MA)

and other research Institutes.

The major work of my latest position was involved in

purification of allergenic protein, characterization of

protein by SDS-PAGE, Western blot, Iso-electric focusing,

Liquid chromatography (HPLC), Mass-Spec analysis,

MALDI-TOF, conjugation of small molecules, biotinylation,

monoclonal antibody based assay development and mass

spectrum analysis of protein and small molecules. The major

work was oriented on innovation of new molecules for

diagnostic assays. The new molecules are developed in R&D

and l transferred to manufacturing unit for large scale

production. In process of knowledge transfer, I wrote batch

records, SOPs, executive report and other necessary

documents. I make multiple products following manufacturing

processes including cGMP, cGLP, cGDP, etc.

The development of new molecule involves collaboration with

other departments. I collaborated with different departments

for i.e. antibody development, molecular biology, protein

chemistry group, etc. to develop new molecules for assays.

The recombinant antigen development and purification of

recombinant antigens, monoclonal antibody development for

ELISA assay, detection of conjugation by MALDI-TOF

analysis, etc. as involved in my work.

I am looking for opportunities to achieve further

experience. I feel that my background expertise would match

well for a research scientist position, R&D group,

Biochemical or biochemistry group, or similar in your

company. I hope my expertise on diagnostic products,

protein, antigen, antibody and related molecular diagnosis

would be useful for your company. I would like to use my

expertise and skills for the development of your company.

I hope to get your responses soon.

Thank you,

Moon Sen, PhD

Los Angeles, California

Email: ********@*****.***

Ph : 501-***-**** (cell)

R E S U M E

Curriculum Vitae

4543 West 104 street, Inglewood, CA 90304

E-mail: ********@*****.***

Phone: 501-***-**** (C)

Moon Sen

Position: Scientist, R&D, Siemens, Los Angeles, CA.

Present Location: Los Angeles, California

Company/Industry: Any. Diagnostic or Similar,

Company Size: Any Location preference: USA.

Professional Position:

2007- 2012: Siemens, Healthcare Diagnostics, Los Angeles,

CA: Biotinylation of protein for assay development of

Immulite 2000. A small and large scale protein purification

and characterization of protein by Gel filtration, Ion

exchange, HPLC, Mass-Spec, MALDI-TOF, Purification of

monoclonal antibody, polyclonal antibody, AP conjugation,

developing monoclonal antibody as tool for molecular

diagnosis.

RESEARCH EXPERIENCES IN CHRONOLOGY:

2006- 2007: Post doc Fellow, Pedi/GI department, MGH,

Harvard Medical School, Boston, MA.

. Mucosal Immunity, mechanism of allergy and molecular

immunology.

. TLR-9 and TLR-7 mediated signaling pathways to study

cross-talk between innate versus adaptive immunity.

2004-2005: Research Fellow, Immunogenetics Laboratory,

Department of Immunology, Mayo Clinic, Rochester,

Minnesota.

. Establishment of allergy model on HLA-class II

transgenic mice.

. Superantigens (Staphyllococcal enterotoxins) mediated

Toxic Shock on transgenic mice models.

. Auto-immune Diabetes (type 1 Diabetes) on endogenous

MHC class II knock-out mice models.

2000-2003: Post Doctoral Fellow, Department of Biochemistry

and Molecular Biology, University of Arkansas for Medical

Science; Post Doctoral Fellow: Department of Pediatrics,

Allergy, and Immunology, ACHRI; Little Rock, Arkansas.

. Structure of peanut protein allergens.

. Purification of major peanut allergens and subsequent

characterization.

. Chemical modifications of allergens and

structure-function studies of allergens.

. Isolation of T cells for the T-cell proteomics and

DNA micro-array analysis for epitope mapping.

EDUCATION:

DEGREE INSTITUTION AND LOCATION

YEAR(s) FIELD OF STUDY

Post Doctoral Fellowship MGH, Harvard Medical School,

Boston, MA, 2000 -2006 Molecular

Immunology; Protein Structures and

Mayo Clinic, Rochester, MN.

Purifications;

characterization; Transgenic mice

UAMS - Univ. of Arkansas for

Medical Sciences, Little models to

study allergy and molecular immunology.

Rock, AR.

ACHRI - Arkansas Children's

Hospital & Research

Institute, AR.

Ph.D. Bose Institute, Jadavpur

University, Kolkata, India 2001 Allergy,

Aeroallergens, Pollen allergy, fungal

spore allergy,

Protein Purification, Immunology,

B.Sc. (Honors), M.Sc. Visva Bharati University,

Santiniketan, India 1993, 1995 Life

Science (Specialization in Biochemistry and

Molecular Biology)

TECHNICAL SKILLS:

Biochemical:

. Protein Purification (Native): major protein from

peanut (Ara h1 and Ara h2 allergens) by ion exchange

chromatography..

. Recombinant Protein Purification: rPru av 1 (allergen

from Cherry) using conventional chromatography. Purification

(recombinant proteins of Honey Bee Venom (i.e. Api m1, Api

m2, Api m 3, Api m5) purified by IMAC (Metal chelating

column Chromatography).

. Antibody Purification: Purification of IgG

(polyclonal and monoclonal mAb by Protein A column).

Affinity Purification: Monoclonal Antibody based protein

purification (nPhl p1, nPhl p5) from Grass pollen allergens

. Gel coupling: Conjugation of Sepharose resin to

(Streptavidin, Serum, mAb/pAb, etc.)

. Characterization of protein by HPLC, GC, MS,

MALDI-TOF, Mass- Spec.

. Sequence analysis of protein/ peptide fragments.

Circular Dichroism (CD) for structure analysis of

allergenic protein.

. Biotinylation of protein, quantification of biotin

assay.

. Biochemical Analysis: SDS-PAGE, Western blot, Dot

Blot, IEF, 2D gel

Immunochemical techniques -

. ELISA, Western blot, Dot-blot, Enzyme immunoassays,

ELI-Spot assays, Cytokine assay - IL4, IL10, IL12p40, etc.

. Isolation of CD4-T, CD8 -T-cells.T cell

proliferation. Isolation of B cells and spleen cells using

MACS & Auto-MACS system

. FACS analysis. V? analysis of MHC class II transgenic

mice.

Molecular- Genomic and plasmid DNA isolation, PCR, cloning,

. Cloning of GAD65 and Insulin gene in pCDNA3 and pIG

vectors for the expression of autoantigens for diabetes

disease. Transformation.

. Animal models - for immunotherapy. Intranasal and

intra-peritoneal allergen challenge, gavage feeding, BAL

for neutrophil, eosinophil, lavage isolation, tail bleed,

etc.

Cell culture -Isolation of Dendritic Cells, Cell culture

. PBMC isolation from mice blood. Measuring blood

glucose for diabetes level in NOD, IL10o, mice.

. Microscopy: a) High resolution LM; b) SEM; c)

Fluorescence

. Computer and Software knowledge: basic Knowledge on

word processing and graphics.

. Experience on identification of airborne including

pollen and molds

Industrial Skills: Contribute to GDP, GLP, GMP Compliance

environment. Writing Batch Records (BRs), Standard

Operating Procedures (SOPs) and other regulatory

techniques..

1995-2000: Junior Research Fellow, Bose Institute, Kolkata,

India.

. Aeroallergens and Human Health (a project funded by

the Ministry of Environment and Forests, Government of

India).

. Research staff in a British Council sponsored higher

education link project (1995-1997) between Bose Institute,

India and Rothamsted Experimental Station, UK

(Environmental biopollution in relation to human and plant

disease).

SCIENTIFIC RECOGNITION: Academic Awards and Honors

. Received the award from the Center of Excellence of

Federal Clinical Immunological Society (FOCIS) for "Novel

Research" in 2006.

. ST*AR award recipient for the outstanding article on

Immunotherapy from American Academy of Allergy and

Immunology, 2006.

. Awarded "FIT fellow award" for best abstract on

Immunogenetics by American Academy of Allergy, Asthma, &

Immunology, 2006.

. Recipient of "Fellow- In-Trainee" recognition award

by American Academy of Allergy, Asthma, & Immunology.

2005-2007.

. Awarded for outstanding article by Clinical

Immunological Society (CIS) in PID consortium, 2006.

. Recipient of Young Scientists Award in the field of

'Medical & Veterinary Section' in 86th Indian Science

Congress held at Chennai, India from 3-7 January 1999.

. Selected as a Research Staff in the higher education

link project sponsored by the British Council between IACR,

Rothamsted Experimental Station, U.K. and Bose Institute,

Kolkata, India in 1995.

PUBLICATIONS:

Rajagopalan, G. Sen, M.M. Singh M, et al and David CS.

2006. Intranasal exposure to staphylococcal Enterotoxin B

elicits an acute systemic inflammatory response. Shock.

25(6):647-656.

Rajagopalan G, Sen M, Manisha Singh, et al and David CS.

2006. HLA-DQ polymorphism and Immune Response to Bacterial

Superantigens - A Study Using HLA-DQ Transgenic Mice.

Infect Immunity.

Rajagopalan G, Sen M and David CS. 2004. In vitro and In

vivo evaluation of Staphylococcal Superantigen peptide

antagonists. Infect Immunity 72(11):6733-37.

Sen M, Kopper R, Pons L, Abraham EC, Burks AW and Bannon

GA. 2002. Protein Structure plays a critical role in peanut

allergen stability and may determine immunodominant IgE

binding epitopes. J Immunol.169: 882-7.

Kopper RA, Odum NJ, Sen M, Helm RM, Stanley JS and Burks

AW. 2004. Peanut Protein Allergens: The effect of Roasting

on Solubility and Allergenicity. Int.Arh. Allergy Immunol.

136(1):16-22.

Kopper RA, Odum NJ, Sen M, et al and Burks WA. 2004. Peanut

protein allergens: gastric digestion is carried out

exclusively by pepsin. J Allergy Clin Immunol 114

(3):614-18

Adhikari A, Sen M, Gupta-Bhattacharya S and Chanda S. 2004.

Incidence of allergenically significant fungal aerosol in a

rural bakery of West Bengal, India. Env. Int. 29:1071-78.

Sen M, Bhattacharya S. and Chanda, S. 2003. Airborne rice

pollen and pollen allergen in an agricultural field:

aerobiological and immunochemical evidence. J. Env.

Monitoring 5:959-962.

REFERENCES: Available upon request.

PROFESSIONAL MEMBERSHIPS:

. American Academy of Allergy, Asthma, & Immunology,

USA, 2002 -present.

. Sigma Xi, USA - 2005 to present

. Life member of ASIOA (American Scientists of Indian

Origin in America), USA

. Life member of Indian Science Congress Association,

Calcutta, India

. Life member of Indian Aerobiological Society, Delhi,

India

OTHER QUALIFICATION / INTERESTS:

1. Trained as Yoga Teacher (RYT, certified from USA Yoga

Schools)

2. Therapeutic Yoga (certified from USA)

3. Certified in Herbal Medicine.

Contact this candidate