Curriculum Vitae

James L. Thomas

Ph.D.

Curriculum Vitae

Curriculum Vitae

James L. Thomas

Ph.D.

Professor of Pharmacology

Division of Basic Medical Sciences

Department of Obstetrics & Gynecology

Office Address Division of Basic Medical Sciences

Mercer University School of Medicine

1550 College Street

Macon, Georgia 31207

CONTACT 478-***-**** (office)

478-***-**** (lab)

478-***-**** (fax)

abo1ms@r.postjobfree.com

Education

Ph.D. 1981 University of Alabama at Birmingham (UAB)

Major: Pharmacology, Minor: Biochemistry

Advisor: Raymond H. Lindsay, Ph.D.

B.A. 1971 Emory University, Atlanta, GA

Major: Chemistry

POSTGRADUATE TRAINING

1996 -1997 Macromolecular Structure, Department of

Biochemistry, Washington University Graduate

Division of Biology and Biomedical Sciences

(4.0 hr audit)

1993 1994 Laboratory on DNA Manipulation,

Department of Biology, Washington University

(4.0 hr credit)

1981 1985 Postdoctoral Research Associate

Department of Obstetrics & Gynecology

Washington University School of Medicine

St. Louis, Missouri

Mentor: Ronald C. Strickler, M.D.

FACULTY APPOINTMENTS

2010-presentProfessor (with tenure)

Division of Basic Medical Sciences and

Department of Obstetrics and GynecologyMercer University School of Medicine

Macon, GA

2006- 2010Associate Professor (with tenure)

Division of Basic Medical Sciences and

Department of Obstetrics and GynecologyMercer University School of Medicine

Macon, GA

2000 - 2005Assistant Professor (tenure-track)

Division of Basic Medical Sciences and

Department of Obstetrics and GynecologyMercer University School of Medicine

Macon, GA

19912000Research Assistant ProfessorDepartment of Obstetrics and GynecologyWashington University School of Medicine

St. Louis, MO

RESEARCH APPOINTMENTS

2007-presentDirector of Research LaboratoriesMercer University School of Medicine

Macon, GA

19851991Research InstructorDepartment of Obstetrics & GynecologyWashington University School of Medicine

St. Louis, MO

19811985Postdoctoral Research AssociateDepartment of Obstetrics & GynecologyWashington University School of Medicine

St. Louis, Missouri

19721973Electron microscopy technicianDepartment of AnatomyEmory University School of Medicine

1966 1968 American Cancer Society Student Traineeship

Summers Division of Endocrinology

Professional Societies

The Endocrine Society, Society for Gynecologic Investigation, American

Society for Pharmacology and Experimental Therapeutics, American Society for Biochemistry

and Molecular Biology, Sigma Xi.

Research Support

Research Associate with Ronald C. Strickler, M.D., P.I.

National Institutes of Health, "Hydroxysteroid Dehydrogenases

in Human Placental Cytosol", HD15903, 01/01/82 - 12/31/84,

$129,322 (direct costs).

Co-Investigator

National Institutes of Health, "Placental 3B-Hydroxysteroid

Dehydrogenase Isomerase", HD20055, 07/01/85 - 03/31/89,

$183,019 (direct costs).

Co-InvestigatorNational Institutes of Health, "Placental 3B-Hydroxysteroid

Dehydrogenase Isomerase", HD20055, 04/01/89 - 03/31/94,

$573,716 (direct costs).

Principal Investigator

National Institutes of Health, "Placental 3B-Hydroxysteroid

Dehydrogenase Isomerase", HD20055, 12/01/94 - 11/30/99,

$348,181 (direct costs).

Principal Investigator

National Institutes of Health, "Placental 3B-Hydroxysteroid

Dehydrogenase Isomerase", HD20055, 03/01/00 - 02/28/05,

$540,000 (direct costs); $717,625 total costs awarded to Mercer University

on 08/01/00.

Principal Investigator

Medical Center of Central Georgia, Clinical Research Center

Inhibition of Human Type 1 3B-Hydroxysteroid Dehydrogenase

Slows the Growth of Hormone-Sensitive Tumors. 07/01/02 -

6/30/03, $10,000 (direct costs).

Principal InvestigatorMedCen Community Health Foundation Grant

Human type 1 placental 3B-hydroxysteroid dehydrogenase can be inhibited without affecting

the activity of human type 2 adrenal 3B-HSD2.

10/01/03-09/30/04, $18,000 (direct costs).

Principal Investigator

National Institutes of Health, "Placental 3B-Hydroxysteroid

Dehydrogenase Isomerase", CA114717, CA114717, 02/01/05 - 01/31/10,

$630,00 (direct costs); $928,052 total costs awarded to Mercer University.

Teaching

Selected lectures (antithyroid drugs, antipsychotic agents)

in the Pharmacology course at the University of Alabama at

Birmingham Medical, Dental, and Optometry Schools, 1977-80

.

Tutor in the Biomedical Problems Program (Phases: Human Development and Genetics, Renal

(Coordinator), Endocrinology, Hematology).

Resource faculty for Pharmacology (Cancer chemotherapy, antimicrobials),

Direct the research of medical students and Ob-Gyn residents,

Mercer University School of Medicine, 2000 - present

.

Published Articles

4. Thomas JL, Bucholtz KM, Kacsoh B: Selective inhibition of human 3-hydroxysteroid

dehydrogenase type 1 as a potential treatment for breast cancer. J Steroid Biochem Molec

Biol 2011, 125:57 65. http://www.sciencedirect.com/science/article/pii/S0960076010003158

5. Thomas JL, Mack VL, Sun J, Terrell JR, Bucholtz KM: The functions of key residues in

the inhibitor, substrate and cofactor sites of human 3-hydroxysteroid dehydrogenase type 1

are validated by mutagenesis. J Steroid Biochem Molec Biol 2010, 120:192-199.

http://www.sciencedirect.com/science/article/pii/S0960076010002153

6. Thomas JL, Bucholtz, KM, Sun J, Mack VL, Kacsoh B: Structural basis for the selective

inhibition of human 3-hydroxysteroid dehydrogenase 1 in human breast tumor MCF-7 cells.

Mol Cell Endocrinol 2009, 302:174-182.

7. Thomas JL, Mack VL, Glow JA, Moshkelani D, Terrell JR, Bucholtz KM: Structure/function

of the inhibition of human 3-hydroxysteroid dehydrogenase type 1 and type 2 by trilostane.

J Steroid Biochem Mol Biol 2008, 111: 66 73.

8. Thomas JL, Huether R, Mack VL, Scaccia LA, Stoner RC, Duax WL: Structure/function of

human type 1 3-hydroxysteroid dehydrogenase: an intrasubunit disulfide bond in the

Rossmann-fold domain and a Cys residue in the active site are critical for substrate and

coenzyme utilization. J Steroid Biochem Mol Biol 2007, 107:80-87.

9. Pletnev VZ, Thomas JL, Rhaney FL, Holt LS, Scaccia LA, Umland TC and Duax WL: Rational

Proteomics V: Structure-based mutagenesis has revealed key residues responsible for

substrate recognition and catalysis by the dehydrogenase and isomerase activities in human

3-hydroxysteroid dehydrogenase/isomerase type 1. J Steroid Biochem Mol Biol 2006, 101

11. Thomas JL, Boswell EL, Scaccia LA, Pletnev V and Umland TC: Identification of key

amino acids responsible for the substantially higher affinities of human type 1 3-

hydroxysteroid dehydrogenase/isomerase (3-HSD1) for substrates, coenzymes and inhibitors

relative to human 3-HSD2. J Biol Chem, 2005, 280:213**-*****.

12. Thomas JL, Umland TC, Scaccia LA, Boswell EL and Kacsoh B: The higher affinity of

human type 1 3-hydroxysteroid dehydrogenase (3-HSD1) for substrate and inhibitor steroids

relative to human 3-HSD2 is validated in MCF-7 tumor cells and related to subunit

interactions. Endocrine Res, 2004, 30

Strickler RC: Site-directed mutagenesis identifies amino acid residues associated with the

dehydrogenase and isomerase activities of human type I (placental) 3B-hydroxysteroid

dehydrogenase/isomerase. J Steroid Biochem Molec Biol, 1998; 66:327-334.

23. Thomas JL, Evans BW, Strickler RC: Affinity radiolabeling identifies peptides

associated with the isomerase site in human type I (placental) 3B-hydroxysteroid

dehydrogenase/isomerase. Biochemistry 1997; 36:9029-9034.

24. Thomas JL, Nash WE, Strickler RC: Physiologic 3B-hydroxy-5-ene-steroid substrates

bind to 3B-hydroxysteroid dehydrogenase without the prior binding of cofactor. J Steroid

Biochem Molec Biol 1996; 58:211-216.

25. Thomas JL, Frieden C, Nash WE, Strickler RC: An NADH-induced conformational change

that mediates the sequential 3B-hydroxysteroid dehydrogenase/isomerase activities is

supported by affinity labeling and the time-dependent activation of isomerase. J Biol Chem

1995; 270:210**-*****.

26. Nash WE, Mercer RW, Blanco G, Strickler RC, Mason JI, Thomas JL: Over-expression of

human type I (placental) 3B-hydroxy-5-ene-steroid dehydrogenase/isomerase in insect cells

infected with recombinant baculovirus. J Steroid Biochem Molec Biol 1994; 50:235-240.

27. Thomas JL, Nash WE, Crankshaw MW, Strickler RC: Affinity labeling in the presence of

the reduced diphosphopyridine nucleotide, NADH, identifies peptides associated with the

activities of human placental 3B-hydroxy-5-steroid dehydrogenase/isomerase. J Soc Gynecol

Invest 1994; 1:155-163.

28. Thomas JL, Nash WE, Myers RP, Crankshaw MW, Strickler RC: Affinity radiolabeling

identifies peptides and amino acids associated with substrate binding in human placental

3B-hydroxy- 5-steroid dehydrogenase. J Biol Chem 1993; 268:185**-*****.

29. Milewich L, Shaw CE, Mason JI, Carr BR, Blomquist CH, Thomas JL: 3B-Hydroxysteroid

dehydrogenase activity in the tissues of human fetus determined with 5-androstane-3B,17B-

diol and dehydroepiandrosterone as substrates. J Steroid Biochem Molec Biol 1993; 45:525-

537.

30. Strickler RC, Thomas JL: Affinity labeling identifies histidine at the active site of

human placental 3B-hydroxysteroid dehydrogenase/steroid 54-ene-isomerase. Am J Obstet

Gynecol 1993; 168:1216-1222.

31. Thomas JL, Strickler RC, Myers RP, Covey DF: Affinity labeling of human placental 3B-

hydroxy-5-steroid dehydrogenase and steroid -isomerase: evidence for bifunctional

catalysis by a different conformation of the same protein for each enzyme activity.

Biochemistry 1992; 31:5522-5527.

32. Thomas JL, Myers RP, Strickler RC: Analysis of coenzyme binding by human placental 3B-

hydroxy-5-ene-steroid dehydrogenase and steroid 5-4-ene-isomerase using 5'-[p-

(fluorosulfonyl)benzoyl]adenosine, an affinity labeling cofactor analog. J Steroid Biochem

Molec Biol 1991; 39:471-477.

33. Thomas JL, Myers RP, Rosik LO, Strickler RC: Affinity alkylation of human placental

3B-hydroxy-5-ene-steroid dehydrogenase and steroid 5-4-ene-isomerase by 2-

bromoacetoxyprogesterone: evidence for separate dehydrogenase and isomerase sites on one

protein. J Steroid Biochem 1990; 36:117-123.

34. Doody KM, Carr BR, Rainey WE, Byrd W, Murry BA, Strickler RC, Thomas JL, Mason JI: 3B-

Hydroxysteroid dehydrogenase/isomerase in the fetal zone and neocortex of the human fetal

adrenal gland. Endocrinology 1990; 126:2487-2492.

35. Luu-The V, Lachance Y, Labrie C, Leblanc G, Thomas JL, Strickler RC, Labrie F: Full

length cDNA structure and deduced amino acid sequence of human 3B-hydroxy-5-ene steroid

dehydrogenase. Molec Endocrinol 1989; 3:1310-1312.

36. Thomas JL, Myers RP, Strickler RC: Human placental 3B-hydroxy-5-ene-steroid

dehydrogenase and steroid 5-4-ene-isomerase: purification from mitochondria and kinetic

profiles, biophysical characterization of the purified mitochondrial and microsomal

enzymes. J Steroid Biochem 1989; 33:209-217.

37. Thomas JL, Berko EA, Faustino A, Myers RP, Strickler RC: Human placental 3B-hydroxy-5-

ene-steroid dehydrogenase and steroid 5-4-ene-isomerase: purification from microsomes,

substrate kinetics, and inhibition by product steroids. J Steroid Biochem 1988

placental 17B,20-hydroxysteroid dehydrogenase affinity alkylated by estrone 3-

bromoacetate: topographic studies with 16-bromoacetoxy-estradiol-17B 3-methyl ether.

Biochemistry 1985; 24:5361-5367.

42. LaRochelle MC, Thomas JL, Strickler RC: Reactivation of human placental 17B,20-

hydroxysteroid dehydrogenase: affirmation of affinity labeling principles. Steroids 1984;

43:209-217.

43. Thomas JL, LaRochelle MC, Covey DF, Strickler RC: Inactivation of human placental

17B,20-hydroxysteroid dehydrogenase by 16-methylene estrone, an affinity alkylator

enzymatically generated from 16-methylene estradiol-17B. J Biol Chem 1983; 258:11500-

11504.

44. Thomas JL, Strickler RC: Human placental 17B-estradiol dehydrogenase and 20-

hydroxysteroid dehydrogenase: studies with 6B-bromoacetoxy-progesterone. J Biol Chem 1983;

258:1587-1590.

45. Pittman JA Jr, Thomas JL, Dale RC, Dailey G, Beschi RJ, Kontzen FN: Thyroidal

radioiodine uptake values in euthyroid subjects in Birmingham, Alabama. Ala J Med Sci

1969; 6:46-51.

Peer-reviewed abstracts: 65 (not shown)

.

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