Assistant Research Medical Experience .Net Project
Resume:
gunasekar manoharan
Email: abo0j5@r.postjobfree.com
Address: ****, **** ******, ***-*, ...
City: Carlsbad, California, 92008, USA
State: CA
Zip: 92008
Country: USA
Phone: 760-***-****
Skill Level: Entry
Salary Range: 5
Willing to Relocate
Primary Skills/Experience:
See Resume
Educational Background:
See Resume
Job History / Details:
GUNASEKAR MANOHARAN, Ph.D, 0011 760-***-****
2825, hope avenue, apt-3, abo0j5@r.postjobfree.com
Carlsbad, California, 92008, USA
PROFESSIONAL PROFILE
Graduate Research Assistant, University of Central Lancashire, Preston, UK. 2011- present
Investigated time-course effects of Momordica charantia extract on cell viability in various cancer cell lines.
Investigated the dose dependent effects of Momordica charantia extract and, momocharin on cell viability in various cancer cell lines and the combined effects of Momordica charantia extract and, momocharin with temozolomide and vinblastine on cell viability and apoptotic pathways.
Investigated the modes, cellular, and molecular mechanisms of actions of Momordica charantia extract and, momocharin in inducing cancer cell death.
Investigate the dose-dependent toxic effects of the vinblastine, cisplatin and temozolomide on cancer cell viability using different types of cancer cells using confocal microscope.
Identified the dose-dependent cytotoxic effects of Momordica charantia extracts and vinblastin on the viability of glioma cells using proliposome formulations.
Developed and validated by UV and Reverse phase HPLC method for the simultaneous quantification of Levocetirizine and Pseudoephedrine in tablet and syrup form and Nebivolol and Amlodipine in tablet form.
Identified the different elements and the level of the in cell culture medium which is uptake by the cancer cells before and after anti cancer drug treatment and was measured using ICP-MS.
Transferred technology to our development partners, thus conserving in-house resource for new projects. Supervised and led a number of successful research projects with three temporary employees over 1 year.
Formulation R&D, Dr. CL Analytical lab Chennai, India. (2006-2007)
Developed once daily controlled release dosage forms for antibiotics.
Supervised tablet production and optimized production parameters for hygroscopic drug
Molecules.
Technical expertise
Cell Biology
Animal tissue culture - Handling and maintenance of cell culture
Sub-cellular organelle fractionation and isolation
Microscopy techniques - Fluorescent/Confocal microscopy
Cell proliferation and cell viability assays
Soft agar assay, clonogenic assay, and matrigel invasion assay
Transient and stable transfection techniques
Biochemistry and Molecular Biology
Biochemical estimations (DNA, RNA, protein, and various enzyme activities)
PCR, Gradient PCR, RT-PCR, real time PCR, and RT-PCR arrays
Analytical Chemistry
CD, FTIR, DSC, HPLC, LC-MS, ICP-MS, MALDI-TOF MS, and electrophoresis
EDUCATION
Doctorate of Philosophy, Cancer Biology, University of Central Lancashire, Preston, UK, (2008-2011)
"Completed his PhD in less than two and half years with great dedication to research"
Identified the modes and cellular and molecular mechanisms of action of each the crude water-soluble extract of M. charantia and alpha, beta momocharin in inducing cell death by measuring caspase-3 and caspase-9 activities, cytochrome-c release and cytosolic calcium.
Master of Science, Toxicology, University of Strafford, London, UK, (2007-2008)
Master of Science, Pharmaceutical Analysis, The Tamilnadu Dr. MGR Medical University, India, 2006
Bachelor of Science, Pharmacy, The Tamilnadu Dr. MGR Medical University, India, (1999-2003)
RESEARCH / WORK EXPERIENCE
University of Central Lancashire, Preston, UK 2008 - 2011
Teaching Assistant (for Prof. Jaipaul Singh) 2009 - 2011
Assisted undergraduate and graduate students with lab project implementations
Taught basic research principles for summer interns and graduate students
Created research projects and assignments for M.Pharm and M.Phil students
Gave lectures on basic principles of pharmacology and analytical chemistry
ACADEMIC ACHIEVEMENTS
Selected as a member of the School of Pharmacy and Allied Sciences Ambassadors based on academic achievement, strong leadership skills, enthusiasm, and commitment to Uclan.
Research assistantship, University of Central Lancashire, 2008-2011
Stood second in class during undergraduate and graduate study.
Undergraduate scholarship, 1999-2003
PUBLICATIONS
Manoharan G, Singh, J. Medicinal Chemistry of the Anti-Diabetic Effects of Momordica Charantia:
Active Constituents and Modes of Actions. The Open Medicinal Chemistry Journal, 2011, 5, (Supple 2-M2) 70-77.
Manoharan G, Singh, J. Lea RB. Cellular mechanism of action of, momorcharin on cell viability of different cancer cell lines (Communicated to Journal of Cell Biology).
Manoharan G, Singh, J. Cellular mechanism(s) of action of a water-soluble extract of Momordica Charantia in inducing cell death on different cancer cell lines (Communicated to Journal of Cell Biology).
Manoharan G, Singh, J. Beneficial effects and cellular mechanism of action of Momordica charantia in the treatment of cancer (In preparation).
Manoharan G, Singh, J. Effects of Momordica charantia fruit extract with the combination of temozolamide and cisplatin in the treatment of glioma cancer (In preparation).
RESEARCH PRESENTATIONS
Manoharan G, Singh J, and Lea RB. Cellular mechanism(s) of action of a water-soluble extract of Momordica Charantia in inducing cell death on different cancer cell lines. Cellular Signalling, Oxford, UK 2011.
Jaiswal SR, Manoharan G, Ahmed W, and Elhissi A. Treatment of glioma using liposomes Generated from alcohol-based Proliposomes. UKICRS Symposium, Belfast, Northern Ireland 2011.
Manoharan G, Singh J, and Lea RB. Mechanism of action of Momordica charantia and, momorcharin on cell viability of different cancer cell lines. Cellular & Integrative Neuroscience research conference, Birmingham, UK 2010.
Manoharan G, Singh J, and Lea RB. Cellular mechanism of action of alpha beta momorcharin on cell viability of different cancer cell line. Cellular Signalling, Manchester, UK 2010.
Manoharan G, Singh J, and Lea RB. Effects of Momordica charantia fruit extract with the combination of temozolamide and cisplatin in the treatment of glioma cancer. Cellular Neurophysiology, Macclesfield, UK 2010
Manoharan G, Singh J, and Lea RB. Beneficial effects and cellular mechanism of action of Momordica charantia in the treatment of cancer. Science and Technology Graduate School Annual Research Conference, Preston, UK, 2010.
Manoharan G, Singh J, and Lea RB. Effects of Momordica charantia fruit extract in the treatment of cancer. Cellular & Integrative Neuroscience research conference, Cardiff, UK 2009.
Manoharan G, Singh J, and Lea RB. Beneficial effects of Momordica charantia in treatment of cancer. Science and Technology Graduate School Annual Research Conference, Preston, UK 2009.
PROFESSIONAL AFFILIATIONS
American Association of Pharmaceutical Scientists
American Association for the Advancement of Science
Member of the Royal Pharmaceutical Society of Great Britain
Member of the British Toxicology Society
Contact this candidate