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Project University

Location:
San Diego, CA, 92122
Posted:
March 09, 2010

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Resume:

Qi Zhong (Ph.D.) Tel:858-***-****, Email: abne04@r.postjobfree.com

Summary of Qualification

A highly-motivated and detail oriented scientist with an active mind, high

standard of excellence and multi-year extensive working experience; a team

player with analytical and strategic thinking, excellent problem solving

and solid communication skills and multi-task ability

Core Competency

V Expertise in biochemistry, molecular, structural and cell biology

V Proficiency in protein purification and refolding including

centrifugation, filtration and chromatography,

V Proficiency in protein chemistry methodologies including biophysical

characterization of proteins and protein interactions, crystallization

V Experience within the field of cancer research, immunology and protein

engineering

V Strong critical thinking, analytical and organizational skills.

V Ability to independently design and perform experiments, and analyze

results for multiple projects

V Capacity to organize assignments and work within deadlines

V Ability to communicate clearly, and to report and present experimental

results and analysis to internal and external clients

V Ability to work effectively as a team player in a complex, changing

environment

V Team player, intensely committed to success and getting the job done well

in a highly dynamic environment.

V Ability to function in a fast-paced entrepreneurial environment.

Selected Achievements

. Designed protein purification processes

. Constructed hundreds plasmids for structural and functional studies

. Built DNA library to sequence genomes of phages using 454 services

. Expressed genes in mammalian cells, yeast and E.coli

. Created a stable COS cell line expressing GFP-SNX1

. Set up, operated and maintained FPLC system, AKTA and Duoflow

. Purified more than twenty recombinant proteins including:

< four lysin enzymes with lytic activity against a broad range of bacteria

< 10+ SNX proteins

. Purified 100+ milligrams of proteins for NMR and crystallography

. Characterized proteins using physical methods including CD, FTIR

fluorescence, and FPLC

. Performed immunoassay (ELISA, Western blot, immuno-precipitation, immuno-

staining)

. Co-defined the NMR solution structure of PX domain

. Wrote experiment protocols and SOPs

Education

Ph.D. (Biochemistry) Simon Fraser University, Burnaby, B.C., Canada

Title: 2D NMR and FTIR studies on amphipathic peptides in solution and on

lipid membranes

M.Sc. (Biochemistry) Beijing University, Beijing, P.R.China

Title: Purification and characterization of Calmodulin from Peking duck

brain

B.Sc. (Biophysics) University of Science and Technology of China, Hefei,

P.R. China

Working Experience

Sapient Discovery LLC San Diego, California

Scientist 5/1/2009-present

Kent SeaTech Corporation San Diego, California

Molecular Biologist 2/1/2006-1/31/2008

University of California

San Diego, Dept of Medicine S,San Diego, Dept of Medicine San

Diego, California

Staff Research Associate, Project Scientist 5/1/1999-1/31/2006

The Scripps Research Institute, Dept. of Immun., Mol. Biology San Diego,

California

Postdoctoral Fellow 2/1/1997-12/31/1999

University of Sheffield Dept. of Mol. Biology and Biotechnology

Sheffield, UK

Postdoctoral Fellow 9/1/1993-1/31/1997



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