Qi Zhong (Ph.D.) Tel:858-***-****, Email: abne04@r.postjobfree.com
Summary of Qualification
A highly-motivated and detail oriented scientist with an active mind, high
standard of excellence and multi-year extensive working experience; a team
player with analytical and strategic thinking, excellent problem solving
and solid communication skills and multi-task ability
Core Competency
V Expertise in biochemistry, molecular, structural and cell biology
V Proficiency in protein purification and refolding including
centrifugation, filtration and chromatography,
V Proficiency in protein chemistry methodologies including biophysical
characterization of proteins and protein interactions, crystallization
V Experience within the field of cancer research, immunology and protein
engineering
V Strong critical thinking, analytical and organizational skills.
V Ability to independently design and perform experiments, and analyze
results for multiple projects
V Capacity to organize assignments and work within deadlines
V Ability to communicate clearly, and to report and present experimental
results and analysis to internal and external clients
V Ability to work effectively as a team player in a complex, changing
environment
V Team player, intensely committed to success and getting the job done well
in a highly dynamic environment.
V Ability to function in a fast-paced entrepreneurial environment.
Selected Achievements
. Designed protein purification processes
. Constructed hundreds plasmids for structural and functional studies
. Built DNA library to sequence genomes of phages using 454 services
. Expressed genes in mammalian cells, yeast and E.coli
. Created a stable COS cell line expressing GFP-SNX1
. Set up, operated and maintained FPLC system, AKTA and Duoflow
. Purified more than twenty recombinant proteins including:
< four lysin enzymes with lytic activity against a broad range of bacteria
< 10+ SNX proteins
. Purified 100+ milligrams of proteins for NMR and crystallography
. Characterized proteins using physical methods including CD, FTIR
fluorescence, and FPLC
. Performed immunoassay (ELISA, Western blot, immuno-precipitation, immuno-
staining)
. Co-defined the NMR solution structure of PX domain
. Wrote experiment protocols and SOPs
Education
Ph.D. (Biochemistry) Simon Fraser University, Burnaby, B.C., Canada
Title: 2D NMR and FTIR studies on amphipathic peptides in solution and on
lipid membranes
M.Sc. (Biochemistry) Beijing University, Beijing, P.R.China
Title: Purification and characterization of Calmodulin from Peking duck
brain
B.Sc. (Biophysics) University of Science and Technology of China, Hefei,
P.R. China
Working Experience
Sapient Discovery LLC San Diego, California
Scientist 5/1/2009-present
Kent SeaTech Corporation San Diego, California
Molecular Biologist 2/1/2006-1/31/2008
University of California
San Diego, Dept of Medicine S,San Diego, Dept of Medicine San
Diego, California
Staff Research Associate, Project Scientist 5/1/1999-1/31/2006
The Scripps Research Institute, Dept. of Immun., Mol. Biology San Diego,
California
Postdoctoral Fellow 2/1/1997-12/31/1999
University of Sheffield Dept. of Mol. Biology and Biotechnology
Sheffield, UK
Postdoctoral Fellow 9/1/1993-1/31/1997