DR. AMER NAVEED QURESHI

*** **********, ******** *******, **

+44(0)791-***-****, +44(0)126*-******

abn5jp@r.postjobfree.com

British Citizen

http://uk.linkedin.com/pub/amer-qureshi/2a/188/b49

Profile A cell culture specialist/molecular biologist/protein chemist

with 18 years of extensive research experience in the field of molecular

biology, cell culture, protein purification, cell based assay development,

ex vivo/ in vivo gene expression and gene modulation, including 6 years'

experience in GLP/GMP work working for a CMO. Keen to explore venues for

suitable application.

Key Skills

ELISA: In vitro diagnostic assay design: Antibody production: Antibody

titration: Protein qualitative and quantitative analysis:

Immunohistochemistry assays: Electrophoresis, agarose and poly-acrylamide:

Western, Southern and Northern blotting: FACS: Enzyme Kinetics: Drug

development: Cell transformation, transfection and transduction: Lentivirus

construction, production and concentration: Tissue culture and Cell

Factories and fermenters: Cell based assay design for bioactivity: Con-

focal microscopy: Industrial grade maintenance and running of lab, GLP/GMP:

Protein Structural Studies: Gene isolation: Gene expression: Gene

sequencing (Sanger and Next Gen High throughput): Designing

oligonucleotides/primers and Blast searches: Gene purification: Molecular

cloning: PCR/RT-PCR: DNA exo/endo nuclease digestion: Vector modification:

Promoter control: Ribozyme engineering: Bioinformatics: Proteomics:

Multiplex qPCR: Global gene expression analysis: Genotyping and

homozygosity: SNP arrays: Protein & Enzyme purification and related

techniques: AKTA machines: 10L Fermenter: EHDJ: Ultra-filtration:

Spectrophotometry techniques: Protein crystallization: RNA and DNA

labelling: Electron microscopy: HPLC: Column chromatography using size,

charge and affinity resins: Atomic Force microscopy: MS Office (Excel,

Word, PowerPoint, Project, Visio), SigmaPlot, SoftMax Pro, Corel Draw,

Adobe CS5

Work Experience

June 2012 - To date Senior Molecular Biologist, Analytical Department,

AMGEN (BIOVEX), UK

- QC testing of viral production batches under GMP

- Targeted sequencing

- Residual Host Cell Quantification

- Development of QC Analytical Assays

March 2011 - January 2012 Senior Research Scientist R & D DEPARTMENT,

SCIPAC (a CMO), UK.

- Expression and purification of recombinant human Leukemia Inhibitory

Factor (rh LIF) from Pichia based expression system using upto 40L

fermenter.

- Construction of expression system for human Interleukin 2 receptor

alpha (IL2ra) in E. coli.

- Development and managing in-house cell based assay systems to

determine the bio-activity in terms of effective dose at 50% (ED50)

for in-house manufactured products like rh EGF, FGF-1, FGF-2, and LIF.

- Conducting a feasibility study for the expression of Aspergillus

oryzae FAD dependent Glucose Dehydrogenase (FADGDH) in Pichia.

- QC the products manufactured. ELISA based quantification of products.

- Attended 'Introduction to ISO 13485' course.

March 2009 - Feb. 2011 Research Associate REPRODUCTION AND ENDOCRINOLOGY,

DIABETES RESEARCH GROUP, KING'S COLLEGE LONDON, UK.

- Genotype analysis using Affymetrix 50K SNP data to look for pancreas

agenesis in a consangious family.

- Pre-amplify precious cDNA samples, and qPCR 674 gene messages to

create a gene expression profile in different human foetal tissue

samples.

- Construction of lentivirus to express transcription factors for trans-

differentiation of non-beta cells to beta cells.

- Expression of oncogenes in beta-cells to initiate proliferation.

2004 - Feb. 2009 Research Associate, RANDALL DIVISION, KINGS COLLEGE

LONDON (VIRATIS, a spin-off company), UK, work carried out as a CRO and CMO

for MMI, UK, a pharmaceutical company.

- Drug development for the depletion of HIV-1 receptors in human blood

cells in view to inhibit HIV-1 infection, worked under GLP standards.

Production of antibodies.

- Cell culture using a number of Mouse, Human, and Monkey primary cells

and cell lines.

- Study of regulatory procedure (FDA and EMEA) for evaluation of drug

delivery system to be used for clinical trials.

- Ribozyme expression in isolated Rhesus macaque peripheral blood cells.

- Design and construction of lentiviral system for delivery of

ribozymes.

- Large scale production and titration of lentivirus, GMP standard work.

- Ribozyme expression in isolated Marmoset peripheral blood cells.

- Designing of in vitro diagnostic assay for lentivirus transduction

efficiency, using ELISA and qPCR.

- In vivo studies assessing lentivirus based delivery system in mice and

marmosets.

- Electrohyrodynamic jetting of cells.

- Home Office license holder for work on mice, rats, guinea pigs,

rabbits and marmosets.

- Development of cell and tissue based ELISA and immunohistochemistry

assays for detection and quantification of expression of gene products

and depletion of gene targets.

- ELISA based and FACS based assay development for titration of viral

transduction.

- Cell based assays for antagonist and agonist study for GPCRs

- Production of polyclonal antibodies against proteins of interest and

assay development for its titration.

Oct. 2003- Oct. 2004 Research Scientist RANDALL DIVISION OF CELL AND

MOLECULAR BIOPHYSICS, SCHOOL OF HEALTH AND BIOMEDICAL SCIENCES, KINGS

COLLEGE LONDON, LONDON, UK.

- Isolation of Tubulin from brain.

- Analysis of microtubules for Ferro-electric properties.

- Structural analysis of microtubules with electron microscopy and

atomic force microscopy.

May 1994- August 1996 RANDALL INSTITUE KINGS COLLEGE LONDON, SCHOOL OF

BIOMEDICAL SCIENCES, KINGS COLLEGE LONDON, UK

- Training in Molecular Biology and Protein Chemistry with focus on C -

reactive protein (CRP), Creatine Phosphokinase (CPK), and Lactate

Dehydrogenase (LDH).

- Generation of novel methods for the isolation & purification of CRP,

and different isoforms of CPK and LDH.

- Analysis of CPK isoforms in heart diseases.

- Isolation of tubulin, polymerization and depolymerization studies.

- Production of antibodies against Tubulin (A and B), Kinesin (light and

heavy chain), CRP, CPK (M, MB and B) and LDH and ELISA based

titration.

- Column chromatography techniques using size, charge and affinity

resins.

- Cloning and sequencing of CRP.

- Expression of CRP.

Publications

. Guntermann, C., Murphy, B. J., Zheng, R., Qureshi, A., Eagles P. A.

M., and Nye, K. E., (1999) Human Immunodeficiency Virus-1 Infection

Requires Pertussis Toxin Sensitive G- Protein-Coupled Signalling and

Mediates cAMP Downregulation, Biochemical and Biophysical Research

Communications, Vol. 256, No. 2,, pp. 429-435.

. Eagles, P. A. M, Qureshi, A. N., Jayasinghe, S. N., (2006)

Electrohydrodynamic jetting of mouse neuronal cells. Biochem J 394,

375-8.

. Jayasinghe, S. N., Qureshi, A. N., and Eagles, P. A. M., (2006)

Electrohydrodynamic Jet Processing: An Advanced Electric-Field-Driven

Jetting Phenomenon for Processing Living Cells, Small, 2, 216 - 219.

. Jayasinghe, S. N., Eagles, P. A. M. and, Qureshi, A. N., (2006)

Electric field driven jetting: an emerging approach for processing

living cells, Biotechnology Journal, 1, 86-94.

. Qureshi, A., Zheng, R., Parlett, T., Shi, X., Balaraman, P., Cheloufi,

S., Murphy, B., Guntermann, C., Eagles, P. (2006) Gene silencing of

HIV chemokine receptors using ribozymes and single-stranded antisense

RNA. Biochem J, 394, 511-518.

. Knight, W., (2006) Missing a few brain cells? Print new ones, New

Scientist, 28th January issue, 26.

Teaching Experience

- 2004-2006 Graduate Certificate for Academic Practices (Non-award

Route) Kings College London

- 1994 -2011: Looking after BSc and MSc research projects

- 2001-2011: Running Biochemistry tutorials for Year 1

BSc and BDS students and Pharmacology lecture for MSc students.

Educational Qualification

Jun 2012 - to date Greenbelt Lean Six Sigma Zenith Lean

Training, UK.

Aug. 1996-June 2003, PhD RANDALL CENTRE, KINGS COLLEGE LONDON, SCHOOL OF

BIOMEDICAL SCIENCES, KING'S COLLEGE LONDON, UK

- Title of the thesis: Ribozyme-based systems against chemokine

receptors CCR5 and CXCR4 for inhibiting HIV-1 replication.

- The research areas include: Molecular cloning of HIV receptors like

CCR5 and CXCR4 by RT-PCR; mRNA expression of these molecules;

Generation of antibodies against CCR5, its mutant and CXCR4;

Development of specific ribozymes against the above targets;

Generation of a T7 RNA polymerase expression system in mammalian cells

for the endogenous production of ribozymes; Vector modification using

different mammalian promoters for the expression of genes and

ribozymes.

Sep. 1991- Apr. 1994 MSc INSTITUTE OF CHEMISTRY, UNIVERSITY OF THE PUNJAB,

LAHORE, PAKISTAN

- Major subject: Analytical Chemistry.

- Research Project: Development of new instrumental technique for the

determination of atmospheric ozone.

1988-1990 BSc UNIVERSITY OF THE PUNJAB, LAHORE, PAKISTAN

- Major Subjects: Chemistry, Botany and Zoology

References :

Dr. Peter J. Sargent

Business Development Manager

BBInnovations

Flat 4

71 Eccleston Square

London SW1V 1PJ

Tel: +44 (0-207*-******

Email: abn5jp@r.postjobfree.com

Dr Peter Eagles

Hodgkin's Building,

King's College London University of London.

Guy's Campus,

London Bridge,

SE1 1UL

Tel: +44 (0-20-784*-****

Email: abn5jp@r.postjobfree.com

Dr. Richard Zheng

Director of Knowledge Transfer

University of East London

Docklands Campus,

4-6 University Way, London E16 2RD, UK

Tel: +44(0) 20 8223 2854

Email : abn5jp@r.postjobfree.com

Dr Philip Marsh

Henrietta Raphael Building,

King's College London University of London.

Guy's Campus,

London Bridge,

SE1 1UL

Tel: +44 (0-20-784*-****

Email: abn5jp@r.postjobfree.com

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