EKTA PATEL

*** *** ****** **, *** TB, Pittsburgh, PA - 15220, 412-***-****, abn0k9@r.postjobfree.com

OBJECTIVE

Seeking a full-time position as a Chemist in which my educational background and research expertise can be effectively

utilized to ensure rapid progress on scientific development projects.

EDUCATION

M.S. in Biotechnology, Duquesne University, Pittsburgh, PA. GPA: 3.2 Aug ’11 - Dec ’12

M.Sc. in Biomedical Science, University of East London, London, UK. Sept ’07 - Nov ’09

Diploma in Medical Laboratory Technology, Sardar Patel University, Anand, India. Jun ’06 - July ’07

B.Sc. in Microbiology, Sardar Patel University, Anand, India. Jun ’03 - Apr ’06

LABORATORY SKILLS

• Microbiology: Aseptic methods, Media preparation, Microscopy and cell culture (cell lines and Assays).

• Histology: Freezer inventory - Cryostat, tissue Culture, tissue staining and tissue processing.

• Molecular Biology and Analytical Chemistry: Isolation of total RNA, cDNA synthesis, Real time PCR, SDS-PAGE, gel

electrophoresis, capillary electrophoresis, micro-array, western blot, ELISA, chromatography (HPLC, GLC, TLC, SEC,

Absorption, Ion-exchange, Affinity), In situ hybridization (FISH), Spectroscopy (FTIR), Flow cytometry, Mass Spectrometry

(MALDI, FAB, ESI), Optical Spectrometry, FRET, NMR, Chemiluminescence.

• Serology and Immunology: Biochemical tests in organ failures (mainly liver and kidney), Antigen-antibody reactions,

Radioimmunoassay (RIA), Haemagglutination, Immune assay, Immunophenotyping, Immunologic pregnancy test, Urine

analysis, Fecal analysis, Sputum examination and Montox test.

• Hematology: Complete and differential blood count of WBCs, Platelet count, ESR, Hemoglobin estimation &

Electrophoresis, ABO blood grouping, Compatibility test, Diagnosis of Anemia, Leukemia & Thalassemia.

• Data Analysis: Using MS- Excel and Prism 4 or 5.

PROFESSIONAL EXPERIENCE

LABORATORY RESEARCH VOLUNTEER, INFECTIOUS DISEASES AND MICROBIOLOGY – UPMC, PITTSBURGH, PA. MAY ’11 - PRESENT

• Performing all research aspects of blood and tissue processing (Separation of WBC & RBC, Culture of blood cells,

PBMCs, isolation Staining of Tissue, microscopy and Cryostats), Complete and differential blood count of WBCs, ELISA, RT-

qPCR, RNA isolation, cDNA synthesis, cell culture, performing SDS page, Gel electrophoresis and western blot, Antigen-

antibody reactions, Media preparation, Microscopy and cell culture (cell lines and Assays), Radioimmunoassay (RIA),

Haemagglutination, Immune assay and Immunophenotyping, Chromatography: HPLC, GLC, Ion-exchange chromatography,

Affinity chromatography & absorption chromatography, flow cytometry and mass spectrometry.

INTERN- QUALITY CONTROL, SCHNEIDER’S DAIRY, PITTSBURGH, PA. SEPT ’12 – JAN

’13

• Performing laboratory testing to meet standards set up in PMO, Standard Methods for the Dairy Industry and FDA 2400

methods.

• Responsible for running and interpreting results on the following tests: butterfats; total solids; temperature; appearance;

weights, code date verifications, direct microscopic count (DMC), Appendix N, pH; brix; coffee testing; phosphatase; total

acidity; shelf life verification; flavor; odor. Perform Coliform (stress test), antibiotic tests and Standard Plate Count and final

plate count for bacteria.

• Responsible for auditing CIP chemical concentrations and ensures compliance to company specifications and to

implement the milk quality as per ISO & MMPO standards

• Responsible for reviewing and approving daily, weekly, monthly recording charts for Regulatory, HACCP, and SQF

compliance to ascertain improvements within the lab and plant.

• Maintaining the records as per ISO and FDA requirements.

• Participated in internal Audits to meet cGMP, SOP and OSHA regulations.

INTERN – IN VIVO PHARMACOLOGY, VERTEX PHARMACEUTICALS INC., SAN DIEGO, CA. MAY ’12 - AUG ’12

• Examined the expression changes of pain target genes after injury in the relevant areas of the pain pathway such as the

primary sensory neurons (DRG neurons).

• Investigated whether the changes in the expression of molecules could be used as efficacy biomarkers or to provide

insight into mechanisms underlying pain.

• Performed data evaluation to investigate how pain models can be applied for new drug discovery such as efficacy testing

and exploring the mechanism or compounds.

• Techniques performed: RNA isolation and purification, cDNA synthesis for qRT-PCR and qRT-PCR.

PHARMACY ASSISTANT, BECKTON PHARMACY, LONDON, UNITED KINGDOM. SEPT ’08 - SEPT ’10

• Dispensed over-the-counter medicines, maintained patient records, dosing information and directions for use.

TRAINEE LABORATORY TECHNICIAN, KARAMSAD MEDICAL HOSPITAL, GUJARAT, INDIA. JUN ’06 - JUN ’07

• Timely and accurately diagnosed hematological, serological, biochemical and microbiological of clinical samples.

AWARDS

• Graduate Scholarship, Duquesne University, Pittsburgh, PA.

• Graduate Scholarship, University of East London, London, UK.

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