Project Manager Quality Control
Resume:
Brenda M. Hertzog
Lansdale, PA 19446
abmu09@r.postjobfree.com
PROFILE
Associate Scientist with extensive experience in pharmaceutical and
clinical laboratories including over 10 years working in a regulated, GLP
structure. Career versatility demonstrated by knowledge and techniques in
the areas of Proteomics/Biomarker Discovery, Histology/Molecular Pathology
and Medical Technology. Managed and trained research associates in daily
laboratory operations. Developed and administered contracts involving the
out-sourcing of projects for assay development and analysis. Exceptional
organizational, troubleshooting, management and problem solving skills. MT
A.S.C.P. certified.
RESEARCH SKILLS AND TECHNIQUES
Protein Analysis Immunological Techniques
Enzyme - link immunosorbent assays Immunohistochemistry (single/doubles)
(ELISA)
Protein immunoblotting (western) Histological special stains
Protein isolation Immunohistochemistry Antibody
Pre-absorption
Immunoprecipitation In Situ Hybridization (ISH)
RNA purification Tissue Embedding / Processing / Microtomy
RT-PCR Cell Culture and Cell/tissue chip
Development
SELDI protein analysis (Ciphergen
ProteinChip ) Protein profiling, Ligand
fishing, protein-protein and
compound-protein interactions
PROFESSIONAL EXPERIENCE
Johnson and Johnson, Research and Early Development, Spring House, PA.
1999-2007
Associate Scientist - Target Validation Team (2003 - 2007)
Supported early development projects of therapeutic teams with assay
development and validation in biomarker discovery through the use of
protein based techniques such as western analysis, gel electrophoresis,
immunoprecipitation and ELISA. Managed a team of Research Associates in
Histology to support various therapeutic areas in drug development. Primary
contact that directly worked with legal department to develop quotes and
contracts with both commercial and private outside vendors in order to out-
source work for assay development and analysis. Over saw contacts to
ensure all specifications and time lines were achieved before any payments
were released. Accomplishments include:
Biomarker Discovery
. Developed and validated an ELISA and western protocol for the measurement
of cSLPI, a novel biomarker for chymase activity used in allergic
rhinitis and asthma clinical studies.
. Conducted Protein-Protein interaction studies, characterized receptor-
ligand binding to determine a potential ligand for orphan receptor to
develop it as a new drug target.
. Received Encore Gold Award (2006) for optimization and execution of the
cSLPI assay. Optimized the gel-based assay for the measurement of cSLPI
levels in laboratory and clinical samples along with the method of
collection of saliva samples using the OraSure technology.
MorphoMetric/Histology
. Independently coordinated the MorphoMetric /Histology area of the Target
Validation team by working with the team members to design novel methods
and procedures for incoming projects from various therapeutic teams along
with establishing and overseeing project priority, checkpoints and
timelines.
. Developed new histological procedures and protocols as needed to complete
projects while providing the necessary training to Research Associates to
perform these procedures.
. Worked one-on-one with project champions to help develop and design
scientific experiments to characterize/validate targets.
. Attended Therapeutic Team project meetings to understand the needs and
project objectives and offer insight on where histological techniques
could be used to further research and help obtain established goals.
. Involved in key projects, including Target Validation, Target
Characterization, Assay Development, Biomarker Development and Model
Confirmation.
Page 2 Brenda M. Hertzog
PROFESSIONAL EXPERIENCE - Continued
o Validated the effects of JNJ compounds on DSS induced colitis.
o Validated the efficacy of C1s inhibitor compounds in the rat
glomerular nephritis model.
o Evaluated numerous C-fms models (CIA, xenografts, glomerular
nephritise) and the efficacy of various FMS inhibitor compounds and
their effects on decreasing macrophages.
o Partnered with team project manager in the validation of the
arthrosclerosis model and development of best method to analyze the
levels of arthrosclerosis in the 15LO mouse model and observe the
effects of the agonist in this model.
o Validated high priority projects from other Johnson and Johnson
Research sites including the validation of the Oncology target in
various cancer tumors for Centocor which played a major role in
advancing the target in the pipeline.
o Provided key Histology/Target Validation support for the Metabolic
team involving characterization of a brain protein which lead to
discovering a novel distribution of this protein in other key
areas, defining it as a new drug target.
Research Associate - Target Validation Team (1999 - 2003)
Supported the site's Therapeutic teams through the use of histological
techniques which include processing, embedding, microtomy (paraffin and
frozen tissue), special stains, immunohistchemistry and in situ
hybridization
. Developed and launched the MorphoMetric Antibody
Database Search Engine, providing various J&J Drug
Discovery sites access to antibody information and
antibody tissue distribution.
. Received Impact Award (2001) for efforts in design and application of the
cell chip and constructing an antibody database search engine, which
could be accessed globally.
ST. LUKES HOSPITAL, Bethlehem, PA
1989 - 1999
Histotechnologist (1996 - 1999)
Trained as a Histologist and duties included Microtomy, Special Stains and
Immunohistochemistry all performed under regulated guidelines in the
hospital's Anatomic Pathology Laboratory. Gained experience in working on
an Automatic Microtome.
Medical Technologist (1989 - 1996)
Worked throughout the entire laboratory performing diagnostic tests through
the use of various automated clinical analyzers along with performing the
maintenance and quality control on the instrumentation to meet with
A.S.C.P. regulations and SOPs.
BLOOD BANK OF DELAWARE, Newark, DE
1985 - 1988
Medical Technologist
Responsibilities included typing, antibody screening, serological and
infectious disease testing of donor blood along with the preparation of
various blood components.
ALFRED I. DUPONT INSTITUTE, Wilmington, DE
1982 - 1984
Medical Technologist
Worked in every section of the clinical laboratory with direct
responsibility for the chemistry and microbiology departments.
Chemistry responsibilities included daily patient work-up, quality
control and instrument maintenance. As chief technologist in
Microbiology, handled the daily patient work-up and quality control
along with research projects requested by staff physicians and
surgeons. Reviewed use of new products in the laboratory and the
development of procedures to incorporate products into the daily work
schedule of the microbiology department.
MEDICAL DIAGNOSTIC LABORATORIES, Allentown, PA
1981- 1982
Medical Technologist
Worked throughout the entire laboratory and oversaw the hematology
department. Managed the laboratory, including quality control.
Page 3
Brenda M. Hertzog
EDUCATION
Bachelor of Science, Medical Technology, MILLERSVILLE STATE COLLEGE,
Millersville, PA
Internship, Allentown School of Medical Technology, Allentown, PA
ACCREDITATION
Registered with the American Society of Clinical Pathologists (MT - 144407)
- Medical Technologist
PUBLICATIONS
Manuscripts
1. Michael R. D'Andrea, Patti A. Reiser, Deborah A. Polkovitch, Norah A.
Gumula, Barbara Branchide, Brenda M. Hertzog, Danielle Schmidheiser,
Stanley Belkowski, Myriam C. Gastard, Patricia Andrade-Gordon. "The use
of formic acid to embellish amyloid plaque detection in Alzheimer's
disease tissues misguides key observations". Neuroscience Letters, 342
(2003) 114-118.
2. Michael R. D'Andrea, Patti A. Reiser, Deborah A. Polkovitch, Norah A.
Gumula, Barbara Branchide, Traci Williams-Riles, Danielle Schmidheiser,
Brenda M. Hertzog. "Application of collagenase on paraffin sections to
enhance Collagen IV immunohistochemical detection without distorting
tissue morphology". Biotechnic and Histochemistry, accepted 2003.
3. M. R. D'Andrea, Robert G. Nagele, Norah A. Gumula, Patti. A. Reiser,
Deborah A. Polkovitch, Brenda M. Hertzog, Patricia Andrade-Gordon.
"Lipofuscin and A-beta 42 exhibit distinction patterns in normal and
Alzheimer's disease brains". Neuroscience Letters 323 (2002) 45-49.
Book Chapter
Lou XJ, Belkowski SM, Dixon JM, Hertzog B, Horwitz D, Ilyin SI, Lawrence
D, Polkovitch D,Towers M, D'Andrea MR. Strategies of biomarker discovery
for drug development. Frontiers in Drug Design and Discovery, eds.
Caldwell, D'Andrea, 2006. Pp. 5-22
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