Ju Wang

Mount Sinai School of Medicine Icahn Bldg 10-76, 1425 Madison Ave, New

York, NY 10029

abmrk6@r.postjobfree.com 917-***-**** (cell), http://www.linkedin.com/in/juwang

OBJECTIVE: Biological related position. Open to relocation. Available

immediately.

SUMMARY: . Solid biochemistry, molecular and cellular biology

experimental skills, with 5 years of hands-on experience in a

lab studying Multiple Sclerosis & related neurodegeneration.

. Excellent organizational, teamwork and communication skills.

. Be able to prepare reports, summaries,

presentations of results, protocols, quantitative analyses,

and maintain appropriate documentation of experiments. Be

independent in Experiment design, execution and

interpretation of data.

. Have taken Biostatistics and Statistics in

both undergraduate and graduate courses.

EDUCATION: . M.S. in Biology, Mount Sinai School of Medicine, NYU,

Spring 2010.

. B.S. in Biology, Capital Normal

University, China.

SKILLS: . In vivo, Animal: handling mouse model of

demyelination and neurodegeneration (Cuprizone, lysolecithin,

EAE), genotyping, mouse surgery including stereotaxic

injection, intraperitoneal injection, mini-pump infusion.

. Biochemistry and protein analysis: Western

immunoblot, SDS-PAGE elctrophoresis, spectrophotometry,

protein immunoprecipitation, ELISA, enzyme assays (HDAC

activity assay, MTT apoptosis assay, etc)

. Molecular biology: DNA work, cloning and

sequencing, recombinant DNA technologies, plasmid design and

construction. RNA isolation, q-PCR (Real-time PCR), reporter

gene assay (Luciferase), transient expression assay,

Lenti/Adeno-viral based RNAi (sh,si RNA), ChIP (chromatin

immunoprecipitation, ChIP-on-chip.

. Histology: tissue preparation and cryo-

sectioning, immuno-fluorescence staining and confocal

microscopy. Electron microscopy study of demyelinated axons.

. Cell biology: extensive experiences with primary neuron and glia

cell culture from mouse and rat, magnetic-bead cell

isolation, brain slice cultures, various mammalian cell line

cultures.

. Computer skills and data analysis:

proficiency in data analysis softwares and websites including

Excel, Prism, Powerpoint, Photoshop, SDS, Pubmed, BLAST,

DAVID, experiences with microarray data analysis.

. Instruments: lab automation technologies

such as Stratagene real-time PCR system with 96-well and 384-

well microtiter plates.

EXPERIENCES and RESEARCH:

Research and graduate Assistant at a lab

studying Multiple Sclerosis and related neurodegenerative

diseases. (2006-2010)

. Histone acetylation is involved in the opposing

regulation of Shh and BMP signaling in oligodendrocyte

development and remyelination. New targets of Shh and BMP

signaling in regulating oligodendrocyte development were

identified using combined Microarray analysis and knocking-

down strategy in primary cells. Protein-protein and protein-

DNA interaction studies were used to elaborate the regulatory

mechanism of Shh and BMP signaling in oligodendrocyte

development and remyelination. Worked with Cuprizone and

Lysolecithin demyelination mouse model (2008-present).

. Characterize the role of SWI/SNF complexes and the Brg-

interacting partners. This has lead to the finding of novel

Brg1-involved epigenetic regulation in oligodendrocyte

differentiation. Use microarray data to find the target and

then confirmed by functional over-expression study in primary

oligodendrocyte cells. Mutation of protein, Protein-protein

and protein-DNA interaction were applied in the project

(2007-2008)

. Using Yy1 conditional knockout mice to illustrate the

critical role of YY1 in myelination. Deletion of Yy1 has led

to demylination of axons in CNS and PNS. Further mechanism

was investigated by finding Yy1 downstream genes which are

inhibiting oligodendrocyte differentiation and cell cycle

exit. (2006-2008). Also worked to generate conditional

knockout mice. This work has been published in Neuron, 2007.

. Using enzymatic assays to characterize apoptotic potential

in cancer cell lines deficient in BH3-only proapoptotic

proteins, which leads to the identification of possible

pathways through which BH3-only proteins regulate apoptosis

in cancer cells. (2006)

HONORS:

. Plenary speaker, American Society for

Neurochemistry 40th Annual Meeting, Charleston,

2009.

. Excellent Thesis Research Award at Capital Normal University,

2004.

. University Scholarship for Outstanding Undergrad Students, 4

years in a row.

PUBLICATIONS:

. Wang J., Shen S., Nguyen T., Androulakis

I., Casaccia P. (2009). Opposing roles of BMP4 and SHH in

modulating HDAC activity in neonatal cortical rat

oligodendrocyte progenitor cells. Conference abstract,

Journal of Neurochemistry, Volume 108 Issue s1 - March 2009 -

(1-182).

. . He, Y., Dupree, J., Wang, J., Sandoval,

J., Li, J., Liu, H., Shi, Y., Nave, K. A., and Casaccia-

Bonnefil, P. (2007). The transcription factor Yin Yang 1 is

essential for oligodendrocyte progenitor differentiation.

Neuron 55, 217-230.

. Shen Tian, Ju Wang, Xinfang Chen,

Zhenhong Yuan, Xiushan Yang *, Ethanol production of

immobilized zymomonas mobilis, Acta Energiae Solaris Sinica,

26, 2, Apr, 2005, p 219-231. (Co-first author)

. Xinfang Chen, Shen Tian, Ju Wang, Xuefeng Li,

Yaping Pan, Zhenhong Yuan, Xiushan Yang *, Acclimatization of

strains for increasing ethanol yield from glucose and xylose,

Acta Energiae Solaris Sinica, 26, 2, Apr, 2005, p 215-218.

CONFERENCES ABSTRACTS:

. Wang J., Shen S., Nguyen T., Androulakis

I., Casaccia P. (2009). Opposing roles of BMP4 and SHH in

modulating HDAC activity in neonatal cortical rat

oligodendrocyte progenitor cells. American Society for

Neurochemistry 40th Annual Meeting, (March 7-11), Charleston,

SC.

. . He, Y., Dupree, J., Wang, J., Li, J.,

Shi, Y., Nave, K. A., Casaccia-Bonnefil,P. (2007).

Transcription Factor Yin Yang1 is essential for

oligodendrocyte progenitor differentiation. Society for

Developmental Biology 2007 Mid-Atlantic Regional Meeting

(March 30-31), Princeton, NJ.

. He, Y., Dupree, J., Wang, J., Sandoval,

J., Li, J., Casaccia-Bonnefil,P. (2007). Activation of

histone deacetylase by Yin Yang1 is required for

oligodendrocyte progenitor differentiation. Annual Symposium

Rutgers and UMDNJ Graduate Student Association (February 23)

Piscataway, NJ.

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