University Chemical

Lihua NIe

*** ********** **. ***.** COLUMBUS, OH 43202 USA.

1-614-***-**** (cell), 1-614-***-**** (work)

abmpp4@r.postjobfree.com

TECHNIQUES AND SKILLS

. Molecular biology techniques: Protein expression, purification and

characterization; Protein labeling, PCR, DNA purification and cloning.

. Analytical skills: Flash chromatography, HPLC, FPLC, SDS protein gel,

Western blot, NMR, LC-MS, Fluorescence, UV-Vis, CD and Chemiluminescence

spectroscopy.

. Organic synthesis, purification and characterization.

. Solid phase DNA synthesis.

. Familiar with SciFinder, ChemFinder and Protein Data Bank.

. Excellent with Excel spread sheet, Kaleidagraph, ChemOffice, PyMOL,

pDRAW.

. Excellent in written and verbal communication.

RESEARCH EXPERIENCE

. Postdoctoral associate, Ohio State University, Columbus, OH, USA. 2006-

present.

1. Developed a stop codon scanning method for alanine scanning and

unnatural amino acid scanning.

2. Engineered proteins with natural and unnatural amino acids.

3. Screened the activity of recombined proteins with co-expressed GFPUV

fluorescence.

4. In vivo screened protein-protein interaction with Bpa photo-crosslinking

method.

5. Identified protein binding sites and binding partner with mass

spectrometry.

6. Synthesized several unnatural amino acids and fluorescence dye for

protein engineering and protein labeling.

7. Synthesized several Fmoc-protected oligo-phosphoramidites and two DNA

libraries.

. Postdoctoral fellow, Max Planck Institute for Chemical Ecology, Jena,

Germany. 2003-2005.

1. Tracking the biosynthetic and transportation pathway of defensive

compounds in leaf beetle larva with LC-MS method.

2. Synthesized several glucosides and thioglucosides.

EDUCATION

. Ph. D. of Bio-analytical Chemistry, Institute of Chemistry, Chinese

Academy of Sciences, China.

1. Developed sensitive and selective chemiluminescence method for the

detection of Cysteine in human blood.

2. Synthesized two fluorescence dyes for specific recognition of thymidine

and guanosine.

. M. S. of Analytical Chemistry, Beijing Normal University, China.

1. Developed a very sensitive chemiluminescence system for the detection of

fluorequinolone antibacterial reagents, fleroxacin and Lomefloxacin, in

human blood.

2. Revealed the Fluorescence Resonance Energy Transfer (FRET) mechanism of

chemiluminescence.

. B. S. of Chemistry, Jiangxi Normal University, China.

1. Synthesized and characterized a complex of aminoacetic acid and formic

acid cobalt.

AWARDS

. Alexander von Humboldt research fellowship. (2004-2005)

. Max-Planck institute research fellowship. (2003-2004)

. China Association for Instrumental Analysis award (CAIA). (2003)

SELECT LIST OF PUBLICATIONS

1. Ma, H.; Nie, L.; Xiong, S. (2004): Recognition of Guanine by A Triazine

Fluorescent Probe through Intermolecular Multiple Hydrogen Bonding,

Supramolecular Chemistry 16(5), 311-317.

2. Nie, L.; Ma, H.; Li, X.; Sun, M. (2003): Recognition of Thymine by A

Triazine Fluorescent Probe through Intermolecular Multiple Hydrogen

Bonding, Biopolymers, 72, 274-281.

3. Nie, L.; Ma, H.; Sun, M.; Li, X.; Su, M.; Liang, S. (2003): Direct

chemiluminescence determination of cysteine in human serum using quinine-

Ce(IV) system, Talanta, 59, 959-964.

4. Nie, L.; Zhao, H.; Wang, X.; Yi, L.; Lu, Y.; Jin, L.; Ma, H. (2002):

Determination of Lomefloxacin terbium sensitized chemiluminescence

method, Analytical and Bioanalytical Chemistry, 374, 1187-1190.

5. Nie, L.; Zhao, H.; Wang, X. (2001): Determination of Fleroxacin by Tb3+

Sensitized Chemiluminescence, Chinese Journal of Analytical Chemistry, 29

(8): 910-912.

6. Nie, L.; Zhao, H.; Wang X.; Wang, X. (2001): Study on the interaction of

bovine albumin and fleroxacin by fluorescence method, Journal of Beijing

Normal University (Natural Science), 37, 87-91.

MANUSCRIPTS IN PREPARATION

1. Nie, L.; Lavinder, J.; Magliery, T. J.; A synthetic approach to alanine

scanning.

2. Nie, L; Magliery, T. J.; Bpa scanning YbaK protein for capturing protein-

protein interaction.

CONFERENCE ORAL AND POSTER PRESENTATIONS

1. Nie, L.; Magliery, T.J. Alanine scanning protein with synthetic

approach. 2010 Fall National Meeting of the American Chemical Society in

Boston, MA.

2. Nie, L.; Magliery, T.J. 2010 Central Regional Meeting of the American

Chemical Society in Dayton, Ohio.

3. Nie, L.; Sarkar, M.; Magliery, T.J. The synthesis of Fmoc-TAG

phosphoramidite and its application in solid phase DNA library synthesis,

2008 Central Regional Meeting of the American Chemical Society in

Columbus, Ohio.

4. Nie, L., Lavinder, J., Magliery, T.J.; A synthetic approach to stop

codon scanning mutangenesis. 2008 Fall National Meeting of the American

Chemical Society in Philadelphia, Pennsylvania.

5. Nie, L.; Keventzidis, C.; Magliery, T.J. A synthetic approach to

scanning mutagenesis with photoaffinity labels for identifying protein-

protein interactions, Ohio Collaborative Conference on Bioinformatics,

2007, Miami University in Oxford, Ohio.

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