Analyst Supervisor

KOYUKI TAKENAKA

**** ******* *** *** ** SAN DIEGO, CA (619) koyuki.takenaka@gmail.

921**-***-**** com

QUALIFICATION SUMMARY

Over 5 years of Analytical Experience: over 3 years in industry / 2 years in

Academic Research

2 Year-Academic Research Experience for M.Sc.:

LC-MS-MS (Thermo TSQ Quantum, ESI, Triple Quadrupoles)

Bioanalytical research, including intensive troubleshooting and problem-solving

with the instrument, sample preparation, and method development

9 months in Environmental: GC-MS (Agilent, FID/TIC) with Purge/Trap Autosampler,

Varian Spectr AA

2.5 years in pharmaceutical including polymer characterization:

HPLC (Shimadzu, UV) with GPC, RP, Ion Exchange Columns

UV/Vis, KF, SPE, ELISA, and dissolution tester

Provided assistance and support to start a new QC lab

RESEARCH EXPERIENCE

01/2008 - Graduate Research for Masters of Science in Analytical Chemistry

5/2010 Supervisor: Prof. Dale Chatfield, San Diego State University

Method Development for Analysis of Testosterone / Epitestosterone in

Human Samples Using LC/MS/MS

Worked on a separation method-development of

testosterone/epitestosterone using LC/MS/MS (Thermo TSQ Quantum with

triple quadrupoles, mostly on ESI, some APCI mode), software:

Xcalibur 2.0.6

10 pg/mL LOD and 50 pg/mL LOQ of standard solutions and reconstituted

urine samples were targeted for a faster and more sensitive method

than existing GC/MS/MS methods utilizing derivatization of

sugar-conjugate steroids

Urine sample preparation: enzymatic hydrolysis of steroid conjugates

followed by solid supported liquid-liquid extraction (Hydromatrix)

/SPE (Isolute C8)

Trouble shooting on HPLC and ESI sprayhead, cleaning the capillary

inlet, replacing tubing, fixing clogging, assisting the supervisor

cleaning quadrupoles

Data collection: exporting and organizing data onto excel sheets

Good analytical laboratory practice: accurate and precise

measurements, paying attention on details, good notebook keeping

Literature search and reviews

Pfizer-Academic Industrial Relations: interactions with multiple

chemists to learn the roles of analytical chemistry and mass

spectrometrists in all parts of projects, with career consultations

and advice over 6 months provided by Michelle Tran-Dube, M.S., at

Pfizer

EDUCATION

8/2010 (exp) SAN DIEGO STATE UNIVERSITY

San Diego, CA

M.S. in Analytical Chemistry

Completed coursework in Mass Spectrometry, Separation Science (HPLC,

GC, CE), Intermediate Metabolites, Chemical Thermodynamics,

Electrochemistry, Literature reviews

08/2002 UNIVERSITY OF UTAH

Salt Lake City, UT

B.S. in Chemistry

Completed coursework in biochemistry, organic, inorganic, physical, and

analytical chemistry

KOYUKI TAKENAKA

5757 COLLEGE AVE APT TT SAN DIEGO, CA (619) koyuki.takenaka@gmail.

921**-***-**** com

WORK EXPERIENCE

10/2006 - LABORATORY ANALYST II

06/2007

AGAT Laboratories, Ltd. (http//:www.agatlabs.com)

Grande Prairie, AB, Canada

Technical analyst at organic-trace station of the environmental

division of commercial laboratories

Extraction and analysis of BTEX in soil and water samples using

GC/MS-FID / Quadrupole (Agilent 5975C Series GC/MSD with 6890N GC)

with purge and trap auto sampler (Teledyne Tekmar, AQUATek 70),

Software: ChemStation

Trained on GC, 6890N for higher concentrations

Preparing external, internal, and reference standards including

calibration checks, matrix spikes, sample spikes, method blanks,

surrogates, and retention time markers

Method validation by meeting criteria for recovery percentage of

untreated samples from GLP certified location, controls, duplicates,

and the determination of LOD and levels of uncertainties

Method Validations of metal analysis using Varian Spectr AA

Preparing high traceability of documentations for audits

Writing the first version of SOPs by revising SOPs from another lab

branch with small difference in lab settings

05/2002 - ANALYST I

10/2004

MacroMed, Inc (bought later by Protherics, now acquired by BTG plc)

Sandy, UT

Privately-held company developing and manufacturing proprietary drug

delivery products

Provided assistance and support to start a new QC lab

Completion of quantitative analyses requested by R&D department in

in-vivo and in-vitro studies and stability test in non-GLP

pre-clinical environment

In-vivo analysis of proteins and small molecules using ELISA, or SPE

followed by HPLC

In-vitro analysis of polymers and peptides using HPLC, Karl-Fischer,

UV-Vis spectrometer, viscometer (Brookfield), dissolution tester, pH

meter, freeze-dryer

Shimadzu VP7 HPLC: reverse-phase, GPC (SEC), and ion exchange

columns, method development, calibration

Set up dilutions of standard solutions, sample preparation with

accurate lab notes in non-GLP environment

Frequent successful troubleshoots and maintenance work on multiple

HPLCs

Accurate data collection using Shimadzu VP7 and Excel, and creating

analysis reports

TEACHING EXPERIENCE

08/2007 - TEACHING ASSISTANT

05/2010 (exp) San Diego State University

Supervisory of 6 semesters of general chemistry and analytical

chemistry labs

Class size: 25 students, 1.5 sections per semester

Advisory and troubleshooting of experiments

Supervisory of the lab safety

Teaching concepts of experiments based on the knowledge of each

student

Grading, reports, quizzes, and notebooks

- References available upon a request -[pic][pic][pic]

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