Medical Development

Xiaofeng Zhao, M.D., Ph.D. candidate

*** **** *******, **** **** BSRB, University of Michigan, Ann Arbor, MI

48109

E-mail: abmkd1@r.postjobfree.com Tel.: 607-***-****

QUALIFICATIONS

. Strong background in molecular and cellular biology and in vivo mice

model study.

. Extensive experience in in-vivo study of tumors and blood vessel

formation in genetically modified mice: knock-out, knock-in and

transgenic mice generation; mice breeding and handling; mice anatomy,

surgery and mammary gland transplantation.

. Proficient in various molecular and cellular biology techniques: western

blotting, protein phosphorylation, flow cytometry, and various cell-based

assays (BrdU incorporation, TUNEL, cell migration, adhesion, invasion and

endocytosis assays etc.). Great experience in signal transduction and

mechanism of action studies. Experienced in gene expression and

regulation, biomarker identification in blood vessel formation and tumor

progression.

. Proficient in tissue collection and examination and various cell

isolation and culture: tissue embedding and paraffin or frozen

sectioning; hematoxylin and eosin staining; immunohistochemistry;

immunofluorescence; endothelial cell, mammary gland epithelial cell,

hemotopoitic and mammary gland stem cell isolation and handling; 3-D

primary cell/stem cell culture.

EDUCATION AND ACCOMPLISHMENTS

. Ph.D. candidate in biochemistry, molecular and cell biology, Cornell

University, Ithaca, NY (Aug. 2004 - expected Aug. 2010).

Thesis: Critical roles of the kinase activity and proline-rich motif of

Focal adhesion kinase in angiogenesis and breast cancer.

* Analyzed the roles of FAK kinase activity in endothelial cell survival

and barrier function during embryonic development (X. Zhao et al.,

Journal of Cell Biology, Jun.7, 2010, editorial highlight).

> Generated the first endothelial cell specific FAK kinase-defective

mutation knock-in mouse in the world; isolated and examined mouse

embryos at various stage of gestation; isolated and cultured primary

endothelial cells from adult mice and performed various cell-based

in vitro assays and signaling transduction analysis; studied tumor

angiogenesis by tumor cell inoculation and by utilizing inducible

transgenic mice model.

> Provided the first direct in-vivo demonstration that FAK kinase

activity is important in embryo angiogenesis and endothelium

integrity during embryonic development and tumor progression, but

not essential to endothelial cell survival, which is in contrast to

previous in-vitro based studies and suggests a future direction of

FAK target drug design.

* Analyzed the roles of the 2nd proline-rich motif of FAK in mammary

gland tumorigenesis and metastasis (X. Zhao et al., in preparation).

> Generated the first FAK 2nd proline-rich motif mutation knock-in

mice in the world; examined mammary gland development, mammary

tumorigenesis and metastasis using human breast cancer mouse model;

isolated and cultured mammary gland epithelial and mammary tumor

cells; analyzed various cellular functions in vitro; isolated and 3D-

cultured mammary gland stem cells or cancer stem cells and studied

stem cell specific functions.

> Identified the FAK proline-rich motif as not necessary for mammary

gland development but essential for breast cancer progression and

metastasis, which provides an exciting and convincing evidence for a

perfect drug design target for human breast cancer.

. M.D., Medical School of NanKai Univeristy, Tianjin, China (Sep. 1997 -

Jul. 2004)

Analyzed the role of Lmo2 oncogene in hematopoiesis and angiogenesis

through regulating VEGF and EPO.

M.D. training: Clerkship and internship in The First Central Hospital of

Tianjin, 254 Military Hospital, Tianjin Children Hospital, and Institute

of Hematology & Blood Diseases Hospital, Tianjin, China.

REFERENCES

. Prof. Jun-Lin Guan

Department of Internal Medicine, University of Michigan.

109 Zina Pitcher Place, 3027 BSRB, Ann Arbor, MI 48109

E-MAIL: abmkd1@r.postjobfree.com TEL: 734-***-****

. Prof. Siu Sylvia Lee

Department of Molecular Biology & Genetics, Cornell University

339 Biotechnology Building, Ithaca, NY 14853-2703

E-MAIL: abmkd1@r.postjobfree.com TEL: 607-***-****

. Prof. Bendicht Pauli

Department of Molecular Medicine,Cornell University

C4 161 Veterinary Medical Center, Ithaca, New York 14853

E-Mail:abmkd1@r.postjobfree.com TEL: 607-***-****

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