Medical C

Xiaomin Mu

**** ******* *****, ******** ***** VA 23454

Tel: 757-***-**** (Cell). Email: abmgvh@r.postjobfree.com

Objective

I am looking for a scientist position in the fields of biochemistry,

molecular and cellular biology, gene therapy, and preclinical development

in industrial settings. My objective is to develop new assays and new

therapeutic products using my skills and experiences to meet the medical

health requirement.

Career Summary

Over 10 year working experience of molecular biology, gene construction,

cell biology and in vivo animal disease models; found androgen receptor and

its related orphan receptors-mediated intracellular signal pathways and

transcription and regulation mechanisms in spermatogenesis and prostate

cancer initialization and progression; developed a cell-based CD4-gp120

mammalian two-hybrid system and a CD4-gp ELISA binding assay for screening

microbicide inhibitors; developed a semen biomarker for microbicide

validation.

Technical Skills

. Tremendous experience in all kinds of hybridization probe design and

labeling.

( Tremendous experiences in animal and human tissue and section

preparations including paraffin-embedded and frozen tissue sections.

. Tremendous experience in in situ hybridization and FISH,

immunohistochemistry, fluorescence microscope, and in vivo image.

( Excellent cell culture skills and experiences in DNA transfection,

reporter gene assay, flow cytometry, and creation of stable cell line

with DNA transfection.

. Strong background in molecular biology, gene cloning, enzyme digestion,

site-directed mutagenesis, DNA/RNA extraction and purification, PCR, Q-

real-time PCR analysis sequence analysis, genotyping, and gene and

protein expression analysis (proteomics).

. Six-year experience in preclinical development of biomarker. Familiar

with most laboratory instrument operations. Good experience in GMP/GLP

practice (recording keeping), familiar with common bioinformatics tool,

and proficiency in MS Office computer programs including word processing

and spreadsheets.

1

Positions and Experience:

Eastern Virginia Medical School, Norfolk VA 2004-

present

Research Scientist

( Developed a semen biomarker for the clinical trials of CONRAD program.

( Developed a CD4-gp120 mammalian two-hybrid system and a CD4-gp120 ELISA

binding assay for screening inhibitors.

( Discovered mechanisms of how cellulose sulfate inhibits HIV-1 entry into

host cells in vitro.

( Discovered novel tyrosine-phospho-proteins in human maturated sperm.

University of Rochester Medical Center, Rochester, NY

1998-2004

Postdoctoral Research Associate,

( Discovered novel functions of testicular nuclear orphan receptors (TR2,

TR3, and TR4) in the development of mammalian prostate and testis.

( Discovered novel signaling pathways (TR2, TR3 and TR4) important in

prostate cancer.

( Discovered important functions of androgen and its related orphan

receptors in germ cell proliferation and the initiation of prostate cancer.

Institute of Zoology, Chinese Academy of Sciences, Beijing, China

1997-1998

Research Scientist

( Discovered the tissue specific expression of several orphan receptors in

rat and primate testes

( Discovered androgen-dependent novel genes in testes.

( Discovered novel genes whose expressions are regulated by environmental

temperature.

Education:

Ph.D., Biochemistry China Agricultural University, Beijing, China.

B.S., Veterinary Medicine Gansu Agricultural University, Gansu, China.

Publications:

1. Mu XM, Yang L, and Chang C. Stage dependent and androgen inductive

expression of orphan receptor TR4. Biochem. Biophys. Res. Commun. 2006,

341 (2), 464-469.

2. Mu XM, Lee YF, Chen YT, Liu NC, Kim E, Syhr CR and Chang C. Delayed and

disrupted late meiotic prophase and subsequent meiotic divisions of

spermatogenesis in mice deficit in testicular nuclear orphan receptor-4

(TR4). Mol. Cell. Biol.2004, 24 (13): 5887-5899.

3. Mu XM, and Chang C. Orphan receptor TR3 mediates apoptosis through up-

regulating transcription factor E2F1 in human prostate cancer LNCaP cells.

J. Biol. Chem. 2003, 278 (44): 428**-*****.

4.Mu XM and Chang C. TR2 orphan receptor functions as negative modulator

for androgen receptor in prostate cancer cells PC-3. The prostate, 2003,

57 (2): 129-133.

5.Yeh SY, Tsi MY, Xu QQ, Mu XM, Lardy H, Huang KE, Lin H, Yeh SD,

Altuwaijri S, Zhou XC, Xing LP, Boyce BF, Huang MC, Zhang S, Gan L, and

Chang C. Generation and characterization of androgen receptor (ARKO) mice:

in vivo model for the study of androgen function in selective tissues.

Proc. Natl. Acad. Sci. USA, 2002, 99 (21):134**-*****.

6. Syhr CR, Collins LL, Mu XM, Platt KA, and Chang C. Spermatogenesis and

testes are normal in mice lacking the testicular orphan nuclear receptor-

2. Mol. Cell. Biol. 2002, 22 (13): 4661-4666.

7. Hu YC, Shyr CR, Che WY, Mu XM, Kim E, and Chang C. Suppression of

Estrogen Receptor-mediated Transcription and Cell Growth by Interaction

with TR2 Orphan Receptor. J. Biol. Chem. 2002, 277 (37): 33571 - 33579.

8. Collins LL, Lin DL, Mu XM, and Chang C. Feedback regulation between

orphan nuclear receptor TR2 and human papilloma virus type 16. J. Biol.

Chem. 2001, 276 (29): 273**-*****.

9. Mu XM, Liu YX, Colin LL, Kim E, and Chang C. The p53/Retinoblastoma-

mediated repression of testicular orphan receptor-2 in the rhesus monkey

with cryptorchidism. J. Biol. Chem. 2000, 275 (31):238**-*****.

10. Guo C-X, Hu Z-Y, Zou R-J, Mu XM, and Liu Y-X. Expression and regulation

of orphan receptor TR2 mRNA in germ cells of cryptorchid testis in rat and

rhesus monkey. Chinese Science Bulletin, 2000, 45(8):720-725.

11. Guo C-X, Tang T-S, Mu XM Li S-H, Fu G-Q, Liu H, and Liu Y-X. Cloning of

novel temperature-related sequence Tag in rat testis during

spermatogenesis. Biochem. Biophys. Res. Commun. 1999, 258 (2), 401-406.

12.Mu XM, Young WJ, Liu YX, Uemura H, and Chang C. Induction of an intronic

enhancer of the human ciliary neurotrophic factor receptor (CNTFR?) gene

by the TR3 orphan receptor. Endocrine, 1998, 9 (1): 27-32.

13.Mu XM and Liu YX. Localization and expression of TR3 orphan receptor in

mouse testis. Chinese Journal of Physiology (Acta Physiologica), 1998,

50(4): 361-366.

14.Liu K, Liu Y-X, Hu Z-Y, Mu XM, and Chen Y-J. Temporal expression of

urokinase type plasminogen activator, tissue type plasminogen activator,

plasminogen activator inhibitor type 1 in rhesus monkey corpus luteum

during the luteal maintenance and regression. Mol. Cell. Endocrinol. 1997,

133(2): 109-116.

References:

Available upon request

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