Assistant Software
Resume:
Li Jing, Ph.D.
**** ******** ****, *** ***, Los Angeles, CA 90066
Phone: 706-***-**** E-mail: **********@*****.***
SUMMARY
Bioanalytical chemist with special expertise in qualitative and
quantitative analysis of biomolecules using Chromatography and Mass
Spectrometry
HIGHLIGHTS
. 6 years' experience in chromatographic separation and mass
spectrometric characterization of peptides, proteins, proteomes and
organic compounds
. Expertise in HPLC, LTQ-FTICR, Orbitrap, TOF, QqQ, MALDI, ESI, nanoESI,
LC-MS/MS, GC-MS, SPE, 2-D protein gel, FT-IR, AFM, STM, and SEM
. Broad practice with biological sample preparation and handling
. Hands-on experience in protein separation, purification, and peptide
sequencing
. Strong capability in methodology development, validation and
application
. 2 years' experience in JAVA programming for automated data processing
. Practical experience in analog/digital circuit design and
implementation
. Excellent communication with chemists and biologists in
multidisciplinary projects
EDUCATION
Ph.D. in Analytical Chemistry, May 2009, University of Georgia, Athens, GA
B.S. in Chemistry, July 2002, Fudan University, Shanghai, China
PROFESSIONAL EXPERIENCE
Aug. 2009-present, Postdoctoral Scholar, Pasarow Mass Spectrometry
Laboratory, David Geffen School of Medicine at UCLA, Los Angeles, CA
Advisor: Professor Julian Whitelegge
1. Developed novel protocols for enrichment of fatty-acylated, and other
covalently lipidated, peptides from complex mixtures
2. Implemented and optimized top-down and middle-down MS strategies for
detection and characterization of protein lipidation in high-density
lipoproteins (HDL)
3. Applied SMIRP (Subtle Modification of Isotope-Ratio Proteomics) to
bacterial systems to improve the metabolic labeling efficiency and
investigate its application in metabolomics
Aug. 2004-May 2009, Research Assistant, Department of Chemistry,
University of Georgia, Athens, GA
Advisor: Professor Jon Amster
1. Established LC, MS, MS/MS (CAD and ECD) & stable isotope labeling
methods to characterize leucine-responsive regulatory proteins in
archaea
2. Designed and implemented a fully automated Java algorithm for
light/heavy peak identification and peptide quantitation of 15N
labeling proteomics, named N15Tool
3. Improved mass measurement accuracy (sub-ppm) for higher mass peptides
with implementation of SWIFT excitation and stepwise-external
calibration in HPLC-MALDI-FTICR analysis
4. Developed a multi-linear regression software to process stepwise-
external calibration data (software and source code are available for
download at http://amstersgi.chem.uga.edu/html/software.html)
5. Investigated a novel calibration method to correct space charge effect
in FTMS analysis and implemented algorithms for automated calibration
of 15N labeled proteome
6. Developed new procedure and condition suitable for analysis of
membrane proteins by bottom-up shotgun proteomics
Jun. 2004-May 2008, Teaching Assistant, Department of Chemistry,
University of Georgia, Athens, GA
1. Taught general chemistry laboratory course
2. Taught graduate level analog/digital electronics laboratory course
3. Trained and supervised undergraduates in an analytical chemistry
laboratory
HONORS AND AWARDS
University of Georgia Dissertation Completion Award, 2009
University of Georgia Student Travel Award, 2008
University of Georgia Student Travel Award, 2007
PUBLICATIONS
1. Jing, L.; Ruchala, P.; Waring, A.; Whitelegge, J. Highly Efficient and
Selective Enrichment of Lipopeptides using Phase Separation for
Proteomic Analysis of Protein Fatty Acylation. Manuscript in
preparation
2. Jing, L.; Li, C.; Wong, R. L.; Parks, B. A.; Liu, Y.; Porat, I.;
Whitman, W. B.; Zubarev, R. A.; Sharp, J. S.; Amster, I. J. Shotgun
Proteomic Analysis Using Accurate Mass Measurement and Nitrogen
Stoichiometry. Submitted to J. Am. Soc. Mass Spectrom.
3. Jing, L.; Amster, I. J. An Improved Calibration Method for MALDI-FTICR
Analysis of 15N-Metabolically Labeled Proteome Digests Using a Mass
Difference Approach. Submitted to J. Am. Soc. Mass Spectrom.
4. Jing, L.; Amster, I. J. Rapid and Automated Processing of MALDI-
FTICR/MS Data for 15N-Metabolic Labeling in a Shotgun Proteomics
Analysis. Int. J. Mass Spectrom., 2009, 287, 27-31
5. Jing, L.; Li, C.; Wong, R. L.; Kaplan, D. A.; Amster, I. J. Improved
Mass Accuracy for Higher Mass Peptides by Using SWIFT Excitation for
MALDI-FTICR Mass Spectrometry. J. Am. Soc. Mass Spectrom., 2008, 19,
76-81
PRESENTATIONS
1. Jing, L.; Ruchala, P.; Waring, A.; Whitelegge, J. Proteomic Analysis
of Protein Fatty Acylation. 58th ASMS Conference, Salt Lake City, UT,
2010
2. Thangaraj, B.; Ryan, C.; Jing, L.; Souda, P.; Faull, K.; Fromme, P.;
Whitelegge, J. Electron-capture and electron-transfer dissociation for
top-down Fourier-transform mass spectrometry of membrane proteins.
58th ASMS Conference, Salt Lake City, UT, 2010
3. Jing, L.; Amster, I. J. An Improved Calibration Method for the MALDI-
FTICR Analysis of 15N-Metabolically Labeled Proteome Digests Using a
Mass Difference Approach. 57th ASMS Conference, Philadelphia, PA, 2009
4. Jing, L.; Amster, I. J. Software for the Automated Processing of Data
from 15N-Metabolic Labeling Proteomics and Accurate Mass Peptide
Identification. 56th ASMS Conference, Denver, CO, 2008
5. Jing, L.; Li, C.; Wong, R. L.; Amster, I. J. Improvements to Stepwise-
External Calibration in FTICR Analysis by Accurate Mass Measurement.
55th ASMS Conference, Indianapolis, IN, 2007
6. Jing, L.; Amster, I. J. Improvements to Stepwise-External Calibration
in FTICR for Proteomic Analysis by Accurate Mass Measurement. 8th
Atlanta-Athens Mass Spectrometry Discussion Group Symposium (AAMSDG),
Athens, GA, 2007 (Oral presentation)
7. Jing, L.; Liu, Y.; Whitman, W. B.; Amster, I. J. Shotgun Proteomic
Analysis of Lrp regulated Proteins of Methanococcus maripaludis Using
Accurate Mass Measurement and 15N-Metabolic Labeling. 54th ASMS
Conference, Seattle, WA, 2006
PROFESSIONAL AFFILIATION
American Society for Mass Spectrometry (ASMS) - Member since 2005
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