Development Manager
Resume:
Jian (Jim) Zhang
abm9g9@r.postjobfree.com
775-***-**** (Home)
Reno, NV 89521
775-***-**** (Mobile)
February 10, 2010
Proven,Inc.
Re: Scientist, Assay Development
Dear Hiring Manager:
I was pleased to see the posting on indeed.com website for the position of
Scientist, Assay Development. I have more than five years pharmaceutical
industry experience in drug discovery and assay development. Upon reviewing
the attached resume, you will find that I am highly capable of fulfilling
this role and performing the associated functions.
My experience, include:
. Assay Development: Bio-chemical assay (FP Assay) and cell-based assays
(transient expression reporter assay, hTR assay and one-step RT-PCR
Assay).
. Designed, developed, validated and implemented biochemical and cell-
based assays for High- Throughput Screening (HTS) on a robotic
platform.
. Experience in compound library management (>300,000 compounds),
screening formats, compounds acquisition, tracking and stock
preparation.
. Mammalian cell culture skills: experience in handling mortal and
immortal cells (MRC5, Hela and BJ).
. Designed and developed project plans, coordinated workflow from concept
to implementation in robotic and automation system to meet
specifications and milestones.
I would enjoy applying my energy in an exciting and challenging position at
Proven. I am familiar and comfortable with industrial team environments and
enjoy the challenges offered by this type of employment. I am flexible and
easily adapt to changes in project direction.
I look forward to an in-person meeting to discuss how my experience matches
the Scientist of Assay Development position and elaborate on how I can
contribute to the success of your company. Thank you for your time and
consideration!
Sincerely,
Jian (Jim) Zhang
Jian (Jim) Zhang
abm9g9@r.postjobfree.com
10520 kenai Dr.
775-***-**** (Home)
Reno, NV 89521
775-***-**** (Mobile)
Objective
Biochemical and cell-based assay development, compound library management
(>300,000 compounds), mammalian cell culture, high-throughput Screening and
automation in biological/pharmaceutical setting.
Qualifications
More than five years pharmaceutical industry experience in drug discovery
and assay design, development, validation, and automation.
. Designed, developed, validated and implemented biochemical and cell-
based assays for High-Throughput Screening (HTS) on a robotic platform;
excellent problem solving skills in HTS.
. Experience in compound library management (>300,000 compounds), screening
formats, compounds acquisition, tracking and stock preparation.
. Participated in drug discovery hit-to-lead process, which included
selectivity and assays required during Structure Activity Relationship
(SAR) studies.
. Coordinated team of researchers successfully completing complex screening
campaign with aggressive timeline.
. Programmed and validated liquid handlers, plate reader, plate washer and
plate sonicator for precision and accuracy.
. Experience in modern spectroscopic analysis; experience and knowledge in
chromatographic analysis, isolation, separation and purification
techniques.
Professional skills
. Assay Development: Bio-chemical assay (FP Assay) and cell-based assays
(transient expression reporter assay, hTR assay and one-step RT-PCR
Assay).
. Mammalian cell culture skills: experience in handling mortal and immortal
cells (MRC5, Hela and BJ).
. Computer skills: working knowledge of Microsoft Excel and Word with
proficiency in data analysis; experience in software: ChemAxxon,
ChemSoft, ChemOffice, Isisbase/draw, SciFinder Scholar, Gaussian 98.
. Analytical techniques: NMR, FT-IR, TLC, HPLC, GC-MS, UV/VIS.
. Ensured seamless HTS data entry, analysis, archiving and retrieval as
liaison with company research information.
Troubleshoot/evaluated/recommended solutions.
. Instrumentation: (programming, troubleshooting, maintenance, user):
liquid handler (Beckman Coulter Biomek FX with integrated ORCA system);
plate reader (Perkin Elmer EnVision); Plate washer (Biotek EL-415W);
dispenser (Thermoelectron Multidrop 96/384) and Plate sonicator
(Microsonic Systems Hendrix-100).
Work Experience
01/2010 - Present Sierra Sciences LLC Reno,
NV
Assay Development / HTS Consultant
. To design, develop and validate biochemical and cell-based assays for
High-Throughput Screening (HTS) on laboratory automation system.
. To handle the chemical compound library database (310,000 compounds) and
maintain the database of chemical libraries
11/2004 - 01/2010 Sierra Sciences LLC Reno,
NV
Research Scientist / Research Associate
. Designed, developed and validated biochemical and cell-based assays for
High-Throughput Screening (HTS) on a robotic platform; completed HTS
campaign of 200k compound library (biochemical, FP-based assay).
. Participated in drug discovery hit-to-lead process, which included
selectivity and assays required during Structure Activity Relationship
(SAR) studies.
. Performed programming, routine maintenance and troubleshooting of liquid
handlers, plate readers, plate washers, plate sonicator and multidrop
dispensers; managed all HTS related consumables and materials supply.
. Responsible for compound library screening formats, compounds
acquisition, tracking and stock preparation; responsible for HTS data
analysis and data management.
. Prepared all cells for the HTS cell-based assays, including cell culture,
trypsinization and plating cells.
1998 - 2004 University of Nevada, Reno Reno,
NV
Research Assistant
. Identified ligand binding sites in proteins and to study protein: protein
interactions; demonstrated its potential application as photo-cross
linkers.
. Performed modern analytical techniques: HPLC, GC-MS, FT-IR, NMR, UV/VIS
and calculation modeling to characterize various compounds.
. Using, maintenance, and timely updating of laboratory notebooks;
interpreted study data and prepared data reports for assigned projects.
1996 - 1998 Central Michigan University Mt.
Pleasant, MI
Research Assistant
. Developed and synthesized indan derivatives as new chemotherapeutic
agents for the treatment of Pneumocystis Carinii Pneumonia; characterized
their structures by FT-IR, GC-MS and NMR.
. Solved the processing problem of a key intermediate in the synthesis of a
target molecule; examined the reduction of different carbonyl compounds
with various reducing agents.
1990 - 1996 Medical School of Beijing University Beijing,
P.R.China
Research / Development Chemist
. Designed and synthesized norcantharidin for anti-cancer drug development;
developed new isolation and purification techniques to improve the yield
of the final products; implemented efficient procedures for scale-up in
Beijing Fourth Pharmaceutical Company; all work was performed in
compliance with pharmaceutical industry Guidelines.
. Worked in a drug discovery group to formulate a drug candidate containing
Chinese herb extracts and norcantharidin for the treatment of cancer and
hepatitis (Chinese Patent issued); provided support in synthesis,
chromatographic analysis and identification of novel compounds.
Education
Ph.D. in Chemistry, University of Nevada, Reno, 2004
M.S. in Chemistry, Central Michigan University, Mt. Pleasant, MI 1998
B.S. in Pharmaceutical Science, Shenyang Pharmaceutical University, China,
1990.
Patents:
1. William H. Andrews; Laura A. Briggs; Christopher A. Foster; Lancer K.
Brown; Mieczyslaw A. Piatyszek; Federico C.A. Gaeta; Rathnam Chaguturu;
Jian Zhang; Tom Kerley. Compound that Increase Telomerase Reverse
Transcriptase (TERT) Expression and Methods for using the same. US
Application No. 12/270,552
2. William H. Andrews; Federico C.A. Gaeta; Laura A. Briggs; Christopher A.
Foster; Lancer K. Brown; Mieczyslaw A. Piatyszek; Jian Zhang; Jessica
Wheeler; Jesse Graham. Quinoline compounds that Increase Telomerase Reverse
Transcriptase (TERT) Expression and Methods for using the same. Filed
November 2008.
3. Shan, Xibin; Guo, Xulin; Zhang, Jian. Formulation of Agan anti-cancer
and anti-hepatitis preparations containing norcantharidin and Chinese
herbs. Chinese Patent 1087526A.
Poster presentations:
1. L.K. Brown, P. Burke, O. Pawlik, A. Collins, W. H. Andrews, J.
Wheeler, J. Zhang, J. Graham, J. Greeson, B. Karakas, O. McGettrick, M. A.
Piatyszek, L. A. Briggs, C. A. Foster. High Throughput Real Time PCR for
the detection of endogenous hTERT mRNA in a Cell Based Drug Screen, The
Quantitative PCR conference, San Diego, CA, 03/2009.
2. J. Zhang, B. Karakas, L.K. Brown, C. Foster, J. Graham, J. Greeson, W.
H. Andrews, S. Tanglao, H. Mohammadpour, F. Gaeta, M. Piatyszek, L. A.
Briggs, R. Chaguturu. A Cell-Based, High Throughput Screen to Identify
Small Molecule Compounds that Derepress the Telomerase Minimal Promoter in
a Transient Luciferase Expression System. Understanding Aging: Biomedical
and Bioengineering Approaches, Los Angeles, CA, 06/2008.
3. J. Wheeler, S. Tanglao, B. Karakas, T. Lovell, L. K. Brown, W. H.
Andrews, L. Tersteege, P. Burke, O. Pawlik, J. Zhang, H. Mohammadpour, L.
A. BriggsInduction of Telomerase Activity in Various Human Cell Lines by a
Small Molecule Compound, C0057684. Understanding Aging: Biomedical and
Bioengineering Approaches, Los Angeles, CA, 06/2008.
4. L. A. Briggs, M. Piatyszek, L. Brown, T. Kerley, J. Zhang, D. Nguyen,
C. Foster, W. H. Andrews, B. Villeponteau, F. Gaeta, B. Karakas, S.
Tanglao, H. Mohammadpour, R. Chaguturu. Induction of Human Telomerase by a
Small Molecule Drug. 37th ANNUAL MEETING: The Role of Genes, Environment
and Chance in Determining Aging, Boulder, CO, 05/2008.
5. E. Gipson, B. Kavlicoglu, L. Brown, J. Zhang, Y. Lee, D. Nguyen, J.
Fleming, J. Townsend, E. Coelho, W. H. Andrews, R. Ardelean, A. Kishpaugh,
F. Villaluz, D. Monda, R. Chaguturu, S. Fraser, H. Mohammadpour, L. A.
Briggs, C. Foster. Fine Mapping of the Human Telomerase Minimal Promoter by
Mutagenesis and Transient Expression Assay. AACR "Translational Research at
the Aging and Cancer Interface", San Diego, CA, 02/2007.
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