Assistant Engineering

Srinath Jagarlapudi

**** ***** ***** ****, *** **22, San Diego, CA 92122

Ph no:- 678-***-****, abm6j4@r.postjobfree.com

Summary

I am a graduate student from Georgia Institute of Technology with 3 years

of experience as a research associate. My work has been to study signaling

pathways and post-translational modifications such as phosphorylation on co-

regulatory proteins. During my experience, I have been able to work on many

Molecular biology and biochemical techniques such as Western blotting, 2D

electrophoresis, co-IP, qRT-PCR, mutagenesis and protein purification

techniques.

Technical expertise

. Molecular Biology techniques: DNA and RNA isolation, Quantitative real

time PCR (qRT-PCR), Site directed mutagenesis, Transformation, Protein

purification techniques, co-IP, knockdown using siRNA, Western

Blotting, 2-D Electrophoresis, maintaining mammalian cell cultures.

. Bioinformatics Tools: Sequence analysis, 3DProtein Structure analysis

by VMD, Argus lab.

. Computer Expertise: "C programming" and Data structures, Microsoft

Office Suite.

Education

Georgia Institute of Technology

Atlanta, GA, USA

Masters, Biology (GPA: 3.8/4.0)

08/2006-Till

date

. Key Electives: Biochemistry, Foundations of Cell and

Molecular biology, Macromolecular Structures, Cancer

Biology, Macromolecular modeling.

Jawaharlal Nehru Technological University

India

Bachelor of Technology, Biotechnology (GPA: 4.0/4.0)

09/2002-04/2006

. Key Electives: Protein Engineering, Biopharmaceutical

technology, Molecular Pathogenesis, Immunology, Bio-Organic

Chemistry, Metabolic Engineering, Micro-Biology.

o Recipient of "Best Outgoing Student 2002-2006 Batch" Award.

Experience

Georgia Institute of Technology

Atlanta, GA, USA

Graduate Research Associate

08/2006 - Till date

. Studied the functional significance of posttranslational modifications

on a co-regulator protein, p54nrb. Determined how these modifications

regulate the activity of p54nrb in both splicing and transcription.

. Studied the mode of activation of p21-activated kinases (PAK) 1 and 6

in adrenal cortex cells. Also determined the mechanism by which these

kinases regulate CYP17 gene transcription

GVK Biosciences Pvt. Ltd

India

Student Researcher

01/2006

-07/2006

. High throughput screening of Matrix metalloproteinase (MMP) and

Caspase Inhibitors.

. Developed and standardized in vitro assays for MMPs 1-9 and Caspases 3

and 8.

Center for Plant and Molecular Biology, Osmania University

India

Research assistant

04/2005 -07/2005

. Screening of Thermophilic soil bacteria producing enzymes like

protease and chitinase.

Publications / Poster Presentations

. Sewer MB., Dammer EB., Jagarlapudi S., "Transcriptional Regulation

of Adrenocortical Steroidogenic Gene Expression" Drug

Metab Rev. 39(2): 371-88. 2007

. Sewer MB., Jagarlapudi S., "Complex assembly on the human CYP17

promoter" Mol Cell Endocrinol. 2008 Oct 19.

. Presented poster "SF-1 phosphorylation integrates periodic ligand

binding and transactivation" at 2007 Keystone symposia, nuclear

receptor conference.

o Travel Award, 2007 Keystone Symposia Nuclear receptor Conference

. Presented poster "Post translational modifications by PAK6 and CARM1

regulate p54nrb activity" at 2008 Experimental biology conference.

. Presented poster "Differential regulation of CYP17 transcription by

PAKs" at 2009 ASBMB conference.

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