Supervisor Quality Assurance
Resume:
CURRICULUM VITAE
CHRISTOPHER R. SALYER
PO BOX 273
Lakeville, IN 46536
******.**@******.***
Education
M.S., Biological Sciences Dec 2006
Wright State University
Dayton, Ohio
Major: Biological Sciences
Total Credit Hours received: 67.0 (quarter hours)
High Honors: Winter 2005, Summer 2005, Spring 2006,
Summer 2006
B.S., Biological Sciences 2004
Wright State University
Dayton, Ohio
Major: Biological Sciences
Minor: Psychology
Total Credit Hours received: 209.0 (quarter hours)
High Honors: Summer 2004
Diploma 2000
Northmont High School
Clayton, Ohio
Research Interests
My main interests lie in molecular and microbiology and
understanding the molecular determinants of Ribulose-1,5-bisphosphate
carboxylase/oxygenase (RubisCO). An understanding of the structure-function
relationships of a protein requires precise knowledge of the role of the
residues (and regions) that are important for catalysis and stability of
the enzyme. Numerous mutational studies have been performed to investigate
the regions and amino acid residues that influence the catalytic properties
of RubisCO. I am using Synechococcus PCC6301, a cyanobacteria, to study
RubisCO. The promising approach for improving the efficiency of Rubisco is
to investigate residues and regions that are farther from the active site.
My research focuses on two site-directed mutants (A47P and A47G) made at
Alanine-47 in the large subunit of Synechococcus PCC6301 RubisCO. These
mutants are made near the large and small subunit interface in the C-
terminal area of the large subunit which is located far from the active
site.
Professional Experience
Zentis Food Solutions 03/09 to present
2050 N Oak Dr. 40+ hours/wk
Plymouth, IN Ending Pay:
$47,500
Supervisor: Tom Fleming Phone: 574-***-****
You may contact this supervisor
QUALITY ASSURANCE DEPARTMENT LAB SUPERVISOR
Duties: Performed quality control laboratory functions in full compliance
with cGMPs; Supervised the day-to-day activities of analytical technicians,
microbiological technicians and incoming goods technicians; Conducted
appropriate OOS investigations following systematic root cause analysis
approach to identify and implement corrective actions; Managed laboratory
instrumentation to ensure continued compliance to the Quality Program and
cGMPs; Responsible for training and motivation of the laboratories;
Motivated self-starters that can work independently with minimal
superision; Supported other functional areas of the company as required;
Participated in continuous improvement as appropriate; Demonstrated a team
work ethic.
Accomplishments:
Developed strong communication skills
Completed FDA certified Better Process Control School training
Familiarized with LIMS system
Drafted and overhauled new departmental SOP's
KIK Custom Products 02/07 to 03/09
1919 Superior St. 40 hours/wk
Elkhart, IN Ending Pay: $41,820
Supervisor: Nick Proicou Phone: 574-***-**** x2308
You may contact this supervisor
MICROBIOLOGIST
Duties: Performed all required microbiological analysis on raw materials,
bulk batches, and finished products; Performed challenge studies;
Maintained required cultures; Prepared necessary media and reagents;
Maintained records on media and equipment; Worked with outside testing labs
when required; Performed general lab analytical procedures; Performed
environmental sampling and analysis; Trained compounders and warehouse
personnel on proper sampling technique; Trained chemists on micro methods
as needed; Performed all testing in an cGMP compliant manner; and performed
related duties as assigned.
Accomplishments:
Developed strong communication skills
Gained industry experience
Learned new lab techniques such as Celsis Rapid Detection
Peak Foods 08/06 to 12/06
1903 W. Main St. 40 hours/wk
Troy, OH Ending Pay: $15/hr
Supervisor: Ernani Celestino Phone: 937-***-****
You may contact this supervisor
FOOD MICROBIOLOGIST/ QC TECHNICIAN
Duties: Ensured products were produced to meet or exceed consumer
requirements; routinely sampled products to ensure the safety of the
product; conducted analytical tests on incoming raw ingredients as well as
final whipped cream products; performed failure testing; followed GMP's,
HAACP guidelines, and all safety rules explicitly; inspected product;
completed appropriate testing worksheets; communicated with appropriate
personnel; maintained lab and lab equipment; and performed related duties
as assigned.
Accomplishments:
. Developed strong communication skills
. Gained industry experience
. Learned new lab techniques such as viscosity and dry solids testing
Wright State University 08/04 to present
3640 Colonel Glenn Hwy 40+ hours/wk
Dayton, OH Ending Pay: n/a
Supervisor: Dr. Stephanie Smith Phone: 937-***-****
You may contact this supervisor
GRADUATE RESEARCH
Duties: Independently conducted research; conducted data analysis;
prepared reports; performed literature searches; trained other students;
dissemination of research to include poster sessions, presentations, and
manuscripts.
Accomplishments:
. Had my abstract accepted and presented a poster showing my thesis
research at the 2006 Ohio Branch ASM meeting.
. Took on an extremely difficult project (my research was a continuation
of my advisor's PhD Dissertation)
. Worked full-time as a research support technician while still making
time for research my first year
Wright State University 09/05 to 09/06
3640 Colonel Glenn Hwy 20+ hours/wk
Dayton, OH Ending Pay: tuition +
Supervisor: Laura Buerschen Phone: 937-***-****
stipend
You may contact this supervisor
GRADUATE TEACHING ASSISTANT
Duties: Attended lectures; facilitated discussions; prepared lectures and
review materials; held office hours; tutored students; managed and
responded to course-related email; graded assignments; proctored and scored
exams; maintained grading records; maintained course attendance records;
calculated grades; acted as a liaison/mediator between students and the
professor; prepared overheads; reviewed literature; and presented lectures.
Accomplishments:
. Fully funded for the 2005-2006 academic year
. Demonstrated substantial knowledge of subject matter as indicated on
student evaluations
. Developed strong communication and public speaking skills
Wright State University 08/04 to 09/05
3640 Colonel Glenn Hwy 40 hours/wk
Dayton, OH Ending Pay: $28,000
Supervisor: Dr. Dawn Wooley Phone: 937-***-****
You may contact this supervisor
RESEARCH SUPPORT TECHNICIAN
Duties: Supported all aspects of day-to-day operations; participated
directly in research; cleaned glassware; safely handled chemicals;
radiation and biohazards; operated and maintained numerous laboratory
items; performed administrative work; ordered supplies; performed a
complete chemical inventory of the laboratory; photocopied articles; and
established new inventory systems.
Accomplishments:
. Designed and maintained a chemical inventory system
. Designed and maintained a freezer inventory system
. Analyzed gene chip data to compare gene expression
. Learned new techniques such as tissue culture and isolation of total
cellular RNA
Wright State University 02/04 to 08/04
3640 Colonel Glenn Hwy 20 hours/wk
Dayton, OH Ending Pay: $5.15/hr
Supervisor: Dr. Dawn Wooley Phone: 937-***-****
You may contact this supervisor
RESEARCH ASSISTANT
Duties: Cleaned glassware; safely handled chemicals; radiation and
biohazards; operated and maintained numerous laboratory items; performed
administrative work; ordered supplies; performed a complete chemical
inventory of the laboratory; photocopied articles; and established new
inventory systems.
Accomplishments:
. Selected from over 50 undergraduate and graduate applicants
. Gained valuable hands-on research experience as an undergraduate
. Completely upgraded and renovated a BSL-3 facility
Gained experience in the operation of common laboratory equipment
Thesis
Salyer, C.R. 2006. Determining the effect of substitutions at
Alanine 47 in Synechococcus PCC6301 Ribulose-1,5-bisphosphate
carboxylase/oxygenase (RubisCO).
Technical Experience
PCR, electrophoresis, DNA restrictions, mini-prep isolations of
plasmid DNA, Westerns, DNA cloning, plasmid vectors, competent cell
transformation, recombinant screening, site-directed mutagenesis, primer
design, DNA quantitation, DNA sequencing, protein quantification,
bacterial transformation, aseptic technique, sterilization, cell culture,
streak plating, serial dilutions, dilution plating, bacterial staining,
enzyme assays, SDS-PAGE, native PAGE, and molecular modeling.
Publiations
Salyer, C., S. Scott and S. Smith. 2009. Determining the effect of
substitutions at Alanine 47 in Synechococcus PCC6301 Ribulose-
1,5-
Bisphosphate Carboxylase/Oxygenase (RubisCO). Manuscript in
preparation.
Conference Presentations
April 2006, poster The American Society for Microbiology Obio Branch
Spring Meeting, Mason, OH. Determining the effect of
substitutions at Alanine 47 in Synechococcus PCC6301 Ribulose-
1,5-
Bisphosphate Carboxylase/Oxygenase (RubisCO).
Fall 2006, poster Wright State University Biology Department Poster
Presentation, Dayton, OH. Determining the effect of
substitutions at Alanine 47 in Synechococcus PCC6301 Ribulose-
1,5- Bisphosphate Carboxylase/Oxygenase (RubisCO).
Funding and Awards
Wright State University Teaching Assistantship, 2005-2006
Teaching Experience
Teaching Assistant, Wright State University, 2005-2006
Courses Taught [Quarters for each in (x)]
BIO 111: Principles of Biology: Human Biology (Supplemental
Instructor) (1)
BIO 112: Principles of Biology: Cell Biology and Genetics (Lab
Instructor) (1)
BIO 115: Principles of Biological Diversity and Ecology (Lab
Instructor) (2)
Professional Affiliations
American Society for Microbiology 2005-2007
Advanced Training
Completed Bloodborne Pathogens Training-2004
Radiation Safety Training-2004, 2005, 2006
Infectious Waste Training-2006
Lockout/Tagout Training-2006
FDA Certified Better Process Control School Training-2009
Management Skills for First Time Supervisors-2009
Public Service
Science Fair Judge, Chaminade-Julienne Catholic High School-Feb. 2005
Languages
German
References
Nick Proicou
Micrbiology Supervisor
KIK Custom Products
1919 Superior St.
Elkhart, IN 46516
574-***-**** x2309
********@*******.***
Dr. Dawn P. Wooley
Associate Professor
Department of Neuroscience, Cell Biology, and Physiology
Wright State University
3640 Colonel Glenn Hwy
Dayton, OH 45435
****.******@******.***
Terry Nolan
Quality Director
KIK Custom Products
1919 Superior St.
Elkhart, IN 46516
******@*******.***
Contact this candidate