Lirui Guan

**** ******* ** *********: (***) ***-

3327

Baltimore, MD 21209 E-mail: ablvkk@r.postjobfree.com

Educational Background

1. September 2005 - May 2009, M.S. in Chemistry.

Johns Hopkins University, Baltimore, MD

2. September 2002 - December 2005, M.S. in Organic Chemistry.

Auburn University, Auburn, AL

3. September 1997 - July 2001, B.S. in Analytical Chemistry.

Hunan University, Hunan, P. R. China

Research Experience

1. DNA interstrand cross-Link formation by abasic Lesion.

2. Irreversible inhibition of DNA Polymerase ? by an oxidized abasic

lesion.

3. Ethynyl-2'-deoxcytidine as mechanistic probe: fluorescence detection of

DNA synthesis in organism.

4. The synthesis of unnatural nucleoside as DNA cross-links reagent,

prodrug or single nucleotide polymorphism (SNP) detection.

5. A synthetic approach to unnatural bis(amino acid)s utilizing Rh(II)-

stabilized carbenoid.

6. The synthesis of tris(2,6-dihydroxyphenyl)E (E = B, P) and the crystal

structure determination by X-ray crystallography.

7. Development of renewable urea sensor based on a self-assembled

polyelectrolyte layer.

Skills

1. HPLC, UPLC, FPLC, UV-Vis.

2. NMR spectroscopy, ESI-MS, MALDI-TOF mass analysis.

3. Multistep organic synthesis including the preparation and handling of

radioactively labeled compounds.

4. Polyacrylamide gel electrophoresis, SDS-polyacrylamide gel

electrophoresis, column chromatography.

5. Protein expression, preparation, purification and immunoprecipitation.

Enzyme and enzyme inhibition kinetic studies.

6. Solid-phase oligonucleotide synthesis and purification, radioactive

labeling of DNA.

7. Molecular biology techniques for manipulating nucleic acids.

8. Proficient in MS office, including Excel, Word, PowerPoint, etc.

Publications

1. Guan, L.; Greenberg, M. M. "DNA Interstrand Cross-Link Formation by the

1,4-Dioxobutane Abasic Lesion" J. Am. Chem. Soc., 2009, 131, 15225 -

15231.

2. Guan, L.; Greenberg, M. M. "Irreversible Inhibition of DNA Polymerase ?

by an Oxidized Abasic Lesion" J. Am. Chem. Soc., 2010, 132, 5004 - 5005

3. Guan, L.; Greenberg, M. M. "Characterization of 5-Ethynyl-2'-

deoxcytidine as Mechanistic Probe" Nucleic Acids Res. (in preparation)

4. Wu, Z.; Guan, L.; Shen, G.; Yu, R. "Renewable urea sensor based on a

self-assembled polyelectrolyte layer" Analyst, 2002, 127, 391-395.

Presentations

1. "Biochemical effects of 1,4-dioxo-2-phosphorylbutane (DOB), an abasic

lesion resulting from the oxidation of DNA." Guan, L.; Greenberg, M. M.

238th ACS National Meeting in Washington DC. August 18, 2009.

2. "Biochemical effects of DOB, an abasic lesion from DNA" Guan, L.;

Greenberg, M. M. 2nd Annual Mid-Atlantic Frontiers at the Chemistry -

Biology Interface Symposium in Baltimore, MD. May 2, 2009.

Teaching Experience

1. Department of Chemistry, Johns Hopkins University (Baltimore, MD)

1) Introductory Organic Chemistry. Teaching Assistant.

2) Intermediate Organic Chemistry Laboratory. Teaching Assistant.

3) Introductory Organic Chemistry Laboratory. Teaching Assistant.

2. Department of Chemistry and Biochemistry, Auburn University (Auburn, AL)

1) Introductory Chemistry and Organic Chemistry Laboratory. Teaching

Assistant.

Contact this candidate