SANDEEP KAKADE

****, ***** ****, **** ***

Email:ablrx0@r.postjobfree.com

Ames, IA-50010

Ph : 313-***-**** (C)

515-***-**** (O)

CAREER PROFILE

Strong fundamentals in Chemical Engineering, Materials Science,

micro/nano particle synthesis, fluorescence microscopy, cGMP techniques,

single molecule techniques and Drug/Gene Delivery.

EDUCATION

Master of Science: Chemical Engineering

Dec 2010 (expected)

Iowa State University, Ames, IA

Master of Science: Materials Science and Engineering

May 2007

Wayne State University, Detroit, MI

Bachelor of Engineering: Chemical Engineering

July 2002

Bangalore University, Bangalore, India

ACADEMIC RESEARCH

Chemical & Biological Engineering, Iowa State University, Ames, IA

Graduate Research Assistant

Nov. 2008 - Present

A single quantum dot-smFRET based bionano-sensor to monitor the

proteolytic activity of matrix metalloproteinase (MMP 14) on QD-peptide

bioconjugates. For this we used the principles of single molecule

Fluorescence Resonance Energy Transfer (smFRET) and Total Internal

reflection fluorescence microscopy (TIRFM) along with biocompatible, water

soluble quantum dots (CdSe-ZnS) as fluorescent probes for the single

molecule detection and imaging.

Polymeric colloidal handles incorporating biocompatible, water soluble

QDs by layer-by layer self assembly and ligand exchange processes. These

micron sized particles have applications in cellular targeting and imaging,

colloidal handles to carryout force measurements by optical trapping and as

fluorescent probes for experimental fluid mechanics.

Lee Laboratory, Purdue University, West Lafayette, IN

Research assistant

Jun. 2007 - Jul 2008

Development of a rapid and early analyzer using the magnetic tweezer

setup for the detection of heart attacks in conjunction with Sensera Inc.

Boston. This project involved fabrication, physical characterization and

surface functionalization of porous alumina membranes for picogram level

detection of cardiac troponin in blood serum. Also surface functionalized

superparamagnetic iron oxide microparticles were used for detection of

cardiac troponin in blood.

Wayne State University, Detroit, MI

Nov. 2005 - May 2007

MS Thesis: DNA containing Layer-by-Layer thin films on colloidal

microparticles for gene delivery

Development of a microcapsule based DNA immunization system using the

layer-by-layer self assembly process. System consisted of biodegradable

polymeric microparticles with layers of poly (ethylene mine) and plasmid

DNA. Physical Characterization using zeta Dynamic Light Scattering and

scanning probe microscopy was achieved. Studies involving transfection and

cellular uptake (using Flow cytometry) of the microcapsules by macrophage

and dendritic cell lines were carried out. HPLC was used to study the

release profile of proteins and drugs from these microcapsules.

Development of an ultrasound assisted perfluorocarbon emulsion droplet

based system, containing polyelectrolyte shells for cancer immunogene

therapy.

PUBLICATIONS

"Physical characterization and transfection activity of layer-by-layer

plasmid DNA/PEI thin films deposited on the surface of biodegradable

microparticles" Sandeep Kakade, Devika S. Manickam, Hitesh Handa, Guangzhao

Mao, David Oupick , Intl. Journal of Pharmaceutics, 2009, 365, 44-52.

INDUSTRIAL WORK EXPERIENCE

Interplex Electronics India Pvt. Ltd

April 2003 - July 2004

Process Engineer

Job profile included Process Control and Optimization, Process

Chemistry, Materials Selection, Design and development activities for the

reduction of loss of precious metal content from the plating baths.

Electrochemical plating, Chemical Etching and Photolithography techniques

for the fabrication of printed circuit boards and electronic components.

Bio-gen Extracts India Pvt Ltd

Aug 2000 - March 2003

Co-operative Intern and Graduate Engineer

Process control and optimization of the solvent extraction process to

enhance the production of drugs from herbal plants. Analytical and

biological purification assays (HPLC and Column) of enzymes and

neutraceuticals. Well conversant in cGMP techniques.

SKILLS

. Laboratory/Research

Analytical techniques (HPLC, Column, UV-VIS spectroscopy, X-Ray

Diffractometry, Atomic absorption Spectroscopy, Dynamic Light

scattering), and polymer chemistry

Microscopy (Light, Fluorescence, Confocal, Scanning Electron microscopy,

Scanning Probe microscopy), biophysical techniques (single molecule

imaging using TIRF and fluorescence resonance energy transfer, Flow

Cytometry)

Micro/nanoparticle synthesis using solvent evaporation, layer-by-layer

self assembly technique.

Cell culture techniques (mammalian), gel electrophoresis.

Microfabrication techniques (photolithography and Chemical etching)

. Computer/Imaging/Data Analysis

Programming Languages: FORTRAN 77, C.

Metamorph Image analysis software, Image J, NIH Image, Adobe Photoshop,

Microsoft Office (word, Excel, PowerPoint) LABVIEW, MATLAB, and MathCAD.

Honors/Awards

. Graduate Student Symposium Poster Competition, First Place, College of

Engineering, Wayne State University, Detroit.

Professional Affiliations

. American Institute of Chemical Engineers (AICHE), & Society of

Biological Engineers (SBE)

. ASM International-The Materials Society

. Biophysical Society.

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