Zeshawn Mumtaz
*** ** *** *******, ***** Miami Beach, FL, 33162
abl5gi@r.postjobfree.com
OBJECTIVE
To obtain a challenging position that will enhance education and provide experience.
EDUCATION
University of Miami, Coral Gables, FL
Bachelor of Science in Biochemistry & Molecular Biology, December 2007
Minor in Biology
EXPERIENCE
Nipro Diagnostics, Inc. Fort Lauderdale, FL
Research & Development Associate, June 2008- Present
• Experience in electrochemistry
o Used single and multichannel potensiostats to perform LOD studies
o Used electrochemical techniques such as chronoamperometry and cyclic voltammetry to asses the potential for new
electrochemical analytes
• Experience with Protein purification
o Purified antibodies using protein A/G columns
o Experience working with dye-ligand chromatography such as cibacron blue dye to isolate BSA from antibodies
o Used dialysis and protein concentrators to perform buffer exchange and concentrate proteins
• Experience with chemical and enzymatic modifications of proteins
o Performed reduction of antibody disulfide bridges in order to create Fab fragments
o Conjugated amine and sulfhydryl containing proteins using maelimide and NHS-ester hetrofunctional cross linkers
o Experienced in creating enzyme label conjugates using glutaraldehyde for glucose oxidase and alkaline phosphatase
o Bound proteins to various solid supports for analysis such as magnetic and latex beads and polystyrene microtiter plates
o Developed methods to conduct immunoassays on nitrocellulose membranes
• Experience with protein detection and quantification
o Used both blue native and SDS-Page gel electrophoresis to detect proteins of interest
o Used Silver staining and Coomaise G-250 dye to detect proteins
o Used a spectrophotometer and or a plate reader to quantify proteins such as Bradford Assay and UV-Vis spectroscopy to
measure chromogenic reagents and or proteins
• Brainstormed and implemented new product design concepts and performed market research
o Proposed vertical flow immunochromatographic design concept for immunoassay
o Designed gold electrodes using a C02 laser
o Researched market reports and gathered data on point of care diagnostic testing and lateral flow testing
o Worked with product development from concept to commercial level feasibility for electrochemical based immunosensor
o Consulted scientific journals on a daily basis for R & D work
• Experience with Tecan automated liquid handling equipment
o Used EVO software to write detailed methods in order to increase throughput and statistical parameters
• Communicated, presented, and archived scientific data for R & D
o Wrote detailed laboratory notebook
o Prepared detailed monthly reports for the directors of both materials and technical development and chemistry
o Presented power point presentations to directors and lab personnel
o Assisted in clinical trials for blood glucose sensors
• Communicated with vendors to help acquire consumable and capital equipment
o Consulted several vendors on automated liquid handling equipment, and on electrophoresis equipment
o Consulted experts in the area of proteomics to improve protein and enzyme biochemistry for current project applications
• Knowledge of FDA, GMP and/or ISO 13485 Quality Systems
o Performed an internal quality audit on materials management for RA/QA
• Basic knowledge of Object-Oriented Programming
o Currently writing software to integrate liquid handling equipment with dated lab equipment
HONORS/ACTIVITIES
• Florida Merit Scholar
• Saxophone Player
• Martial Arts
SKILLS
• Microsoft Word, Excel, and PowerPoint; Web researching skills
• Working knowledge of Spanish