Safety Officer Manager

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Heather J. Hughes

**** ******** **, *******, ** 95648

Cell: 916-***-**** Home: 916-***-**** e-mail: ********@*******.***

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Interests in Veterinary Medicine:

I am interested in small animal and avian/exotic animal clinical veterinary

medicine. In addition, I am very passionate about small animal behavior,

internal medicine, surgery and avian/small mammal medicine as well as

molecular research. One day I hope to combine these interests with my

research goals in my career as a veterinarian by doing both clinical

practice and research. I have worked as a small animal and exotic animal

veterinarian in the Sacramento area following my 2009 graduation from UC

Davis Vet Med. I enjoy working in supportive multi-doctor small

animal/exotic animal practices where I can continue to develop and expand

my clinical veterinary skills to provide personable, high quality medical

care that incorporates diagnostic technology such as ultrasound & digital

radiography. I am committed to quality client education as I firmly believe

a good client-doctor relationship is the cornerstone for quality veterinary

medical care.

Qualifications

o Veterinary Clinical/Laboratory Animal skills:

Veterinary Clinical (canine, feline, psittacine): abdominal

ultrasound expertise, ear cytology, diff-quik staining,

fecal analysis (fresh and float), blood smear analysis,

abdominal ultrasound, standard surgery- spay, neuter, mass

removal phlebotomy, restraint, injections (IP,IM SC, IV,

microchip), TPR, SQ fluid administration, assisted feeding,

radiography, wound & catheter care, ear cleaning, surgery prep,

etc.

Lab Animal: rodent survival surgery (SQ drug pump implants),

venipuncture (tail vein), cardiac stick, brain

perfusion, fat pad analysis, indirect calorimetry, NMR body

analysis, injections (IP, IM, SC), oral gavage,

behavioral feeding assays.

o Highly Computer literate: Familiar with all major MS Windows and

Macintosh based applications, including

Word, Excel, Access, PowerPoint, Outlook, DNA sequence analysis, primer

design, plate reading software, etc.

o Extensive research experience with training in several laboratory

techniques, including:

Molecular Biology: DNA cloning, gel purification, UV

spectroscopy, electrophoresis, bacterial transformation

& screening, DNA PCR, RT PCR, qPCR, sequencing, DNA/RNA

extraction, DNA dot blotting, Southern &

Northern blot, in-situ hybridization, autoradiography.

Protein Chemistry: chromatography, native and SDS PAGE

electrophoresis, Western blot.

Immunochemistry: ELISA, florescence microscopy, radioimmunoassay.

Neurobiology: brain sectioning, punch analysis, immunostaining,

stereotactic drug infusion.

Cell Culture: mammalian culture (rodent cardiac myocytes, PC12,

hippocampal neurons, canine glioma cells),

bacterial expression, long-term bacterial culture propagation.

Organic/Analytical Chemistry: HPLC, ion-exchange chromatography.

Education

o DVM, UC Davis School of Veterinary Medicine. Class of 2009.

o MS in Neuroscience, June 2003. University of California at Irvine,

Irvine, CA.

Thesis- An Inducible Transgenic Model of Dendritic mRNA Targeting.

o BS in Biology, BA Chemistry, August 1996. Seattle Pacific University,

Seattle, WA.

Emphasis in cellular biology, ecology and marine biology.

o CLspMB Certification (Clinical Laboratory Specialist in Molecular

Biology), August 1999.

Clinically certified to perform molecular diagnostic testing in

cancer, paternity and infectious disease.

Publications

o Smith JB, Nguyen TT, Hughes HJ, Herschman HR, Widney DP, Bui KC, Rovai

LE. Glucocorticoid-attenuated response genes induced in the lung during

endotoxemia. Am. J. Physiol. Lung Cell. Mol. Physiol. 2002. 283: 636-47.

o Roth JD, Hughes HJ, Kendall E, Baron AD, Anderson CM. Anti-obesity

effects of the -Cell Hormone Amylin in Diet Induced Obese Rats: Effects

on Food Intake, Body Weight, Composition, Energy Expenditure and Gene

Expression. Endocrinology. 2006. Dec; 147:5855-64.

Employment

Relief Veterinarian, Watt Ave Pet Clinic, Sacramento, CA.

July 2010

to Small animal veterinary care utilizing ultrasound, radiography, in house

present bloodwork and cytological analysis, allergy testing and immunotherapy,

behavior assessment and therapy

Surgery (spay, neuter, etc)

Relief Veterinarian, Northern California Veterinary Specialists,

July 2010 Sacramento, CA.

to

present Emergency small animal veterinary care utilizing ultrasound, radiography,

in house bloodwork and cytological analysis, critical care therapy

Relief Veterinarian, Marysville Veterinary Hospital, Marysville, CA.

July 2010 Small animal veterinary care utilizing ultrasound, radiography, in house

to bloodwork and cytological analysis, allergy testing and immunotherapy,

Aug 2010 behavior assessment and therapy

Surgery (spay, neuter, etc)

Veterinarian, Bird and Pet Clinic of Roseville. Roseville, CA.

Jan 2009 Small animal and exotic veterinary care utilizing ultrasound, radiography,

to in house bloodwork and cytological analysis, allergy testing and

May 2009 immunotherapy, behavior assessment and therapy

Surgery (spay, neuter, declaw, tail amputation, minor wound repair,

enterotomy)

4.5 month externship position

Veterinarian, Jackson Creek Veterinary Clinic. Jackson, CA.

Sept 2009 Small animal and exotic veterinary care including well care (vaccinations,

to new pet/puppy/kitten exams), illness diagnosis and treatment, and after

Nov 2009 hours emergency care

Digital radiography, ultrasound, in house blood panels and cytology in

addition to 'sent out' bloodwork and fecal analysis utilized for diagnosis

Routine surgery (spay, neuter, tail amputation, minor wound repair) and

cystotomy

Part time position

Senior Research Associate, Amylin Pharmaceuticals, Inc. San Diego, CA.

Nov 2003 Metabolic and neurobiological research in obesity and diabetes using a

to rodent diet-induced obesity model

July 2005 In addition to research duties, performed lab managerial tasks such as

ordering, safety officer and training new lab members

Techniques utilized: food intake and body composition monitoring,

real-time pcr, rodent NMR, aseptic rodent survival surgery, indirect

calorimetry, biochemical analysis

Data presented at NAASO (2004); Keystone Symposia (2004, 2005); peer

reviewed journal publication in Diabetes

Veterinary Technician, Midland Animal Clinic, Poway, CA.

Sept 2003

Small animal veterinarian assistance, injections, phlebotomy,

to autoradiography, handling, fecal flotation exams, ear cleaning, nail trims,

July 2005 prescription filling, client education.

Teaching assistant, Dept. of Neurobiology and Behavior, UCI.

Sept 2001 Taught 9 wk undergraduate neuroscience laboratory

to Course content- neural anatomy on rabbit and sheep brains,

June 2003 neuropharmacology on rabbit ileum, pharmacological nigrostriatal lesion

(simulates Parkinson's disease) & behavioral testing on rats, human EEG

In research utilized multiple molecular biology, biochemistry and cell

culture techniques to create an inducible transgenic mouse (PCR, cloning,

sequencing, cell culture, western blot, etc)

Research Associate II, UCLA, Dept. of Pediatrics/Neonatology.

Aug 1999

to Immunology research on chemokine/cytokine expression in the lung in

Aug 2001 response to endotoxiemia

Acting lab manager (ordering, training new lab members, writing protocols)

and safety officer

Techniques utilized: northern blots, gel electrophoresis, DNA, PCR,

cloning, tissue culture, etc.

UC Davis Veterinary School Student Activities:

o Solano County Trap Neuter and Release, Fairfield shelter: Vet Student co-

President, 2007-2008, Still volunteer monthly performing spays and

neuters (approx. 80 spays & 100+ neuters, 1 enucleation, 1 tail

amputation so far), Officer duties: recruiting/organizing vet student

volunteers for monthly feral cat spay and neuter clinic for the positions

of student spay surgeon, student neuter surgeon, spay assistants,

anesthesia team leader, anesthesia assistants and crash tech/neuter

assistant, Additional duties- helping clinic head vet (Dr. Abby

Whitehead) with administration of clinic flow and managing controlled

drug log.

o Canine Medicine Club: Co-President, 2007-2008, duties: listed below &

coordinating other officer responsibilities, ordering/ distributing club

t-shirts.

o Co-Jr. President, 2006-2007 & 2006-2005, duties: in charge of

organizing CMC table at 2007 KCRA health expo, represented club (with

my schnauzer) at the KCRA health expo in Jan 2006; helped plan and

volunteered at CMC annual Davis Picnic Day events; volunteered at

annual CMC symposia & CMC wetlabs, distributed food during food

pickups (vendor food for vet student program).

o Externships: Sacajawea Health Care for Pets, Federal Way, WA (6/25-

7/20/07) & All About Pets, Citrus Heights, CA (9/1-

9/15/09):.Observation/discussion of diagnosis/treatment at a busy 4

veterinarian staffed small animal-exotics veterinary practice that has

been a fixture in the community for 30 years (Dr. Lee Harris, owner (WA),

Dr. Lynda Zucca (CA)). Observed surgery (wide range from routine to

orthopedic including my own cats' thyroidectomy in WA and laser surgery,

dentals, chemotherapy, ultrasound, autoradiography, acupuncture, many

doctor-client interactions and other standard clinic practices.

o 2006 STAR grant recipient (funded via Merck-Merial grant): Summer

research in novel liposomal anti-topoisomerase therapy (CPT-11) for

malignant canine glioma with Drs. Peter Dickinson and Richard LeCouteur

published in 2007

o Attendee 2006 Merck Merial Veterinary Scholars Conference at LSU Vet Med:

presented poster on STAR grant research.

o Mercer Veterinary Clinic for the Homeless: 2006-2007 Co-VP of Volunteers

and Co-VP of Food Donations. Enlisted both student and veterinarian help

for the clinic, organizing and collating patient charts, assisting with

patient exams, obtaining and transporting donated food to the monthly

clinic meeting the veterinary needs of Sacramento's homeless, appeared on

local news show to advertise Christmas Santa Paws fundraiser with

kittens.

o Behavior Medicine Club: 2005-2006 co-Symposium fundraising coordinator &

symposium food coordinator, volunteered at 2006 symposium staffing

registration table as well as attending talks, helped distribute food

during periodic food pickups.

o Veterinary Business Management Association: 2006-2007 co-speaker

coordinator, help bring speakers to educate veterinary students about

good business techniques in preparation for being future successful

practice owners, hosted 2 speakers/quarter (6/yr), attended 2007 NAVC

conference as VBMA officer.

o Avian and Exotics Club: Planned the annual A& E symposia, volunteered in

the symposium at the registration table, volunteered and brought my

parrots, rats, & 3-legged turtle to the clubs' exotic animal display at

2006, 2007, 2008 UC Davis Picnic Day.

o Companion Avian and Exotic Animals (CAPE) treatment crew: 2005-2006

school year,assisted CAPE students, technicians and veterinarians with

care of inpatient animals, typically involves feeding, oral, IM, IP, IV

injections, weighing, TPR, etc

o Feline Medicine Club, Orphan Kitten Project: spring 2006, fostered and

bottle fed two orphan kittens (from 2 wks old), vaccinated, spayed, and

adopted them through the club.

o Annual UC Davis Spay Event Participant: 2006, 2007 served as a

'guardian' for multiple castrated or spayed animals throughout the pre-

exam/induction/surgery/recovery/post-exam process, helped check in and

discharge animals.

References

Available upon request.

Contact this candidate