Plant Manager

Amanda R. Stiles

Department of Plant and Microbial Biology (PMB)

University of California, Berkeley

abkdkc@r.postjobfree.com

EDUCATION

**** – 2007 PhD in Plant Physiology and Molecular Biology, with Dr. Beth

Grabau

Virginia Polytechnic Institute & State University, Blacksburg VA

1995 – 1999 B.S. in Biology / Chemistry minor / Magna Cum Laude

Virginia Polytechnic Institute & State University, Blacksburg VA

RESEARCH EXPERIENCE

8/2007 – present Laboratory Manager / Postdoctoral Scholar, Dept. of Plant and

Microbial Biology, University of California, Berkeley.

9/2002 – 8/2007 Graduate Student, Dept. of Plant Pathology, Physiology, and

Weed Science. Virginia Tech, Blacksburg, VA.

9/2001 – 12/2001 Extended Studies Graduate Student. Dept. of Bioinformatics and

Computational Biology. George Mason University, Fairfax, VA

1/2001 – 9/2001 Technician at Norman M. Cole Pollution Control Plant. Lorton,

VA

1/2000 – 6/2000 Intern at the German Institute for Health and Environment.

Saarbrücken, Germany

9/1998 – 5/1999 Undergraduate Researcher, Dept. of Biological Sciences. Virginia

Tech,

TECHNICAL SKILLS

Molecular Biology

• DNA, RNA and plasmid isolation

• PCR and Quantitative (real-time) PCR

• Cloning using traditional methods and the Gateway™ system

• Recombinant protein expression and characterization

• Northern and Southern blotting

Biochemistry

• High performance liquid chromatography (HPLC)

• Ultraviolet/Visible (UV/Vis) spectroscopy

• Enzyme kinetic assays

• Western Blotting

• Inductively coupled plasma – optical emission spectroscopy (ICP-OES) analysis

• Experience with basis mass spectroscopy analysis using MALDI-TOF, LC/MS,

and GC/MS

• Experience with basic electron scanning microscopy

Plant Biology

• Plant tissue culture initiation and propagation (soybean, peanut, wormwood,

snowlotus)

• Plant transformation techniques (agrobacterium, gene gun)

• Plant cultivation and analysis using indoor growth chambers

• Greenhouse experience (collecting, initiating and propagating plants, setting up

hydroponics)

Computer Skills

• Basic bioinformatics tools

• Website design and maintenance

• Basic programming experience in C++

• Microsoft Office, Open Office, Endnote, Photoshop,

Dreamweaver

TEACHING OPPORTUNITIES

Lecturer: Biotechnology Applications (BCHM/BIOL 4784) – Spring 2006

Instructor: Molecular Biology Laboratory (BIOL 4774) – Spring 2004

GSI: Plant Pathology Laboratory (PPWS 3104) – Fall 2004

JOURNAL ARTICLES (Peer reviewed)

Chamberlain, P., Qian, X., Stiles, A.R., Cho, J., Jones, D., Lesley, S., Grabau,

E.A., Shears, S.B., Spraggon, G. (2007) “Integration of inositol phosphate

signaling pathways via human ITPK1” Journal of Biological Chemistry.

282(38):281**-*****.

Stiles, A.R., Qian, X., Shears S.B., Grabau, E. (2008) “Metabolic and signaling

properties of an Itpk gene family in Glycine max”. FEBS Letters. 582(13):1853-

1858.

Stiles, A.R., Bautista, D., Atalay, E., Babaoğlu, M., Terry N., (2010)

“Mechanisms of Boron Tolerance and Accumulation in Plants: A Physiological

Comparison of the Extremely Boron-Tolerant Plant Species, Puccinellia distans,

with the Moderately Boron-Tolerant Gypsophila arrostil” Environmental Science

and Technology. Epub Aug 10, 2010.

Stiles, A.R., Torabinejad, J., Grabau, E.A., “Late Pathway Genes in Phytic Acid

Biosysnthesis in Glycine max” Manuscript in preparation

REPORTS AND UPDATES (Non-peer reviewed)

Amanda Stiles and Norman Terry (2009) “Long-Term Reclamation of Boron-

Contaminated Soils - First Quarterly Report: February 1 to May 1, 2009”, pp. 1-

21.

Amanda Stiles, Amelia Nguyen, Sindhu Kubendran and Norman Terry (2009)

“Long-Term Reclamation of Boron-Contaminated Soils - Second Quarterly

Report: May 1 to August 1, 2009”, pp. 1-33.

Amanda Stiles, Amelia Nguyen, Henry Tran and Norman Terry (2009) “Long-

Term Reclamation of Boron-Contaminated Soils - Third Quarterly Report: August

1 to November 1, 2009”, pp. 1-13.

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Norman Terry, Amanda Stiles, Priya Padmanabhan (2010) “Biotechnological

Approaches to Removing Boron from Electric Utility Wastewater”, Electric Power

Research Institute, Technical Report: 1021443, pp. 1-54.

Amanda Stiles and Norman Terry (2010) “Long-Term Reclamation of Boron-

Contaminated Soils – Year End Summary of Current Research Status”, pp. 1-7.

Amanda Stiles and Norman Terry (2010) “Long-Term Reclamation of Boron-

Contaminated Soils - Report for Feb 1 to July 31, 2010”, pp. 1-27.

Jung Chen Huang, Matthew Verce, Amanda Stiles, and Norman Terry (2010)

“Development of a Constructed Wetland Water Treatment System for the Salton

Sea Species Conservation Habitat – A Review”, pp. 1-42.

GRANTS WRITTEN AND FUNDED

Identifying Protein-Protein Interactions Involving Soybean Inositol

Phosphate Kinases - Graduate Research Development Project Grant at Virginia

Tech University, 4/1/05

Botanical Drug Development: A Scientific Rationalization of the Process –

University of California Research Bridging Grant, 3/21/08

Use of Boron Hyperaccumulating Plants for the Remediation of Boron

Contaminated Effluents – Electric Power Research Institute, 5/19/08

Developing Novel Procedures for the Long-Term Reclamation of Boron-

Contaminated Soils – Rio Tinto Borax, 9/19/08, Renewed 2010

Biotechnological Approaches to Removing Boron from Electric Utility

Wastewater – Electric Power Research Institute, 11/9/08, Renewed 2010

FUNDING PRESENTATIONS

Once the oil is out of the bay, what do we do with it? (Matter of Trust non-profit,

4/30/08)

Bioremediation of Hydrocarbon Contaminated Mats. (Presidio Trust, 5/12/08)

Bioremediation of Hydrocarbon Contaminated Mats. (Evergreen Oil, 5/16/08)

Remediation of Hydrocarbon Contaminated Mats. (Patagonia, 9/31/08)

ORGANIZATIONAL AND MANAGEMENT EXPERIENCE

Laboratory Management

• Assisted in the reestablishment of the research laboratory of Dr. Norman Terry

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(2007-2010).

• Managed research projects, laboratory setup, training, purchasing, and funding.

• Researched and co-wrote grants to generate funding and re-establish research

program.

• Initiated research projects in several different scientific disciplines (research in

progress).

Mentoring – 2002 - 2010

• Worked intensively to train and mentor undergraduate students, graduate rotation

students and technicians at Virginia Tech University.

• Trained and mentored a team of twelve undergraduate research assistants and

graduate students at the University of California, Berkeley.

Leadership Experience

Berkeley Postdoctoral Association (BPA – Vice President) : 2007 - 2009

• Wrote and distributed bi-monthly newsletters to 1100 Berkeley postdocs.

• Revitalized the website and kept it updated during my tenure at UCB.

• Founded the International Postdoc Visa Committee.

• Initiated and organized a newsletter for postdocs at all 10 campuses (~5,000

postdocs).

University of California Council of Postdoctoral Scholars (Chair) - 2009 - 2010

AWARDS AND HONORS

10/2006 Bruce Perry Fellowship

9/2006 Arthur J. Webber Outstanding Graduate Student Award

3/2006 22nd Annual Research Symposium 2nd place poster (Agriculture

and Animal Sciences Division), Virginia Tech

5/2005 Colonel Horace E. and Elizabeth F. Alphin Graduate Tuition

Scholarship

3/2005 21st Annual Research Symposium 1st place poster (Agriculture and

Animal Sciences Division), Virginia Tech

2005 Inducted into Phi Sigma Biological Honor Society

9/2002 – 5/2005 Virginia Tech Cunningham Fellowship

9/2003 Bruce Perry Tuition Scholarship

9/2002 Bruce Perry Tuition Scholarship

9/1999 – 9/2000 Carl Duisberg Society (CDS) International Student Exchange

Award

1999 Inducted into Phi Beta Kappa

CONFERENCES ATTENDED

Stiles, A.R., Grabau, E.A., 2003, “Identification and characterization of inositol

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1,3,4-trisphosphate 5/6-kinase and inositol 1,4,5-trisphosphate 3/6-kinase in

Glycine max”, 20th Mid-Atlantic Plant Molecular Biology Society Conference

(MAPMBS), August 7-8, 2003, Laurel, MD (as poster)

Stiles, A.R., Grabau, E.A., 2005, “Characterization and Modification of a

Biosynthetic Pathway in Glycine max - Improving the nutritional characteristics of

soybeans”, Lipid-Mediated Plant Signaling Meeting, October 26-29, 2005,

Raleigh, NC (as poster)

Stiles, A.R. and Grabau, E.A., 2005, “Characterizing steps in phytic acid

biosynthesis as targets for manipulating inositol phosphate metabolism in

soybean”, Bouyoucos Conference on Inositol Phosphates in the Soil-Plant-

Animal System, August 21-24, 2005, Sun Valley, ID (as poster)

Stiles, A.R., Shears S.B., Xun, Q., Grabau, E.A., 2006, “Role of inositol kinases in

phytic acid biosynthesis”, Molecular and Cellular Biology of the Soybean

Conference, August 5-8, 2006, Lincoln, NE (as poster)

Stiles, A.R., Grabau, E.A., 2006, “Generating a low phytic acid soybean:

Targeting inositol kinase enzymes in Glycine max”, 23rd Mid-Atlantic Plant

Molecular Biology Society Conference (MAPMBS), August 17-18, 2006, Laurel,

MD (as poster)

RESEARCH PRESENTATIONS

Isolation of Inositol 1,3,4-Trisphosphate 5/6-kinase and Inositol 1,4,5-

Trisphosphate 3/6-kinase from Glycine max, January 29, 2004, Blacksburg, VA

Characterization of Late Pathway Genes in Phytic Acid Biosynthesis in Glycine

max, November 3, 2004, Blacksburg, VA (Research Proposal)

Elucidating the Role of Inositol Kinases in Phytic Acid Biosynthesis in Glycine

max, October 19, 2005, Blacksburg, VA (Research Update)

ADDITIONAL SKILLS

Conversant in Spanish and German, learning Mandarin Chinese

Certified Emergency Medical Technician (EMT) and Scuba Diver

Edit scientific papers as an independent contractor for American Journal Experts

Website design and maintenance: http://plantbio.berkeley.edu/~terry

Hobbies: reading, languages, Aikido

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