Project Assistant

**** ******* ***., ******** ***** Cell: 770-***-****

Berkeley abkdaf@r.postjobfree.com

CA - 94702

SUMMARY

. Extensive experience using cellular and molecular biology techniques

in a research environment.

. Independently managed projects and collaborations through decision-

making, multi-tasking, and designing and implementing experiments.

. Able to work in multidisciplinary teams with cross-functional members.

EDUCATION

Northwestern University, Evanston IL, USA

McCormick School of Engineering and Applied Science

(August-2006 - April 2008)

. Master in Biotechnology

JNT University, Hyderabad, India

(August 2002 - June 2006)

. Bachelor in Biotechnology Engineering

RESEARCH EXPERIENCE

Research Analyst

(March 2010 - September 2010)

Plequis, Inc., New Jersey, USA

Research Associate

Department of Biotechnology, GKVK University, Banglore, India

(October 2008 - January 2010)

. Agro Biomass Conversion for Bio Ethanol Production - project 1

. Fermentation medium preparation, equipment cleaning and setup.

Graduate Student, Northwestern University, Chicago, Illinois

(August 2006 - March 2008)

. Investigated the mechanism and specificity of proteasome mediated

degradation

. Measured distance between the unstructured initiation site and

ubiquitinated site of the proteins using fluorescent resonance energy

transfer (FRET).

. Troubleshooting laboratory instruments (PCR, sequencing).

Research Associate, Center for Cellular and Molecular Biology (CCMB),

Hyderabad, India

(June 2002- August 2006)

Human Genome Diversity Project:

. Investigated the distribution of mitochondrial haplogroup in Sudra

population.

. Inferred the population genetic structure by Analysis of Molecular

Variance (AMOVA).

. Performed genetic analysis using the mtDNA markers, which includes

hypervariable segment I (HVSI), 9 bp, mitochondrial single nucleotide

polymorphisms (SNPs), Y-chromosomal markers, which includes SNPs &

seventeen YSTRs.

. Performed pylogenetic analysis (PHYLIP, MEGA) with various types of

data and DNA sequence analysis (editing, assembling and BLAST

searches).

LABORATORY SKILLS

Molecular Biology

. DNA & RNA isolation, Northern and Southern blotting, plasmid vector

construction, sequencing of phage & plasmids, genotyping, DNA sequence

analysis, designed primers for DNA cloning, subcloning, PCR (including

RT-PCR), site-directed mutagenesis, processing of microarrays, large

scale transient transfections, siRNA transfections, electroporation,

flow cytometry.

Cell Biology

. Mammalian cell tissue culture (including T cells & NK cells),

maintenance of tumor cell lines, transfection of mammalian cells,

hybridoma cell expression.

Protein Analysis

. Lysate preparation, immunoprecipitation, electrophoresis (gel or

capillary), SDS-PAGE, Western blotting (Li-Cor Odyssey), capillary

isoelectricfocusing.

Protein Production

. Virus generation; protein expression and purification, production

using chromatography techniques (ion exchange, column, hydrophobic

interaction), characterization of proteins (stability, solubility)

Immunology

. Antibody characterization, immunohistochemistry, immunocytochemistry,

immunogenicity assays

Assays

. Cell based assays, cytotoxicity assays, biochemical assays, reporter

gene assays, ELISA, ELISPOT RIA, FACS, FPLC, HPLC, GC, FRET, cell

based signal transduction assays, cell proliferation assays, enzymatic

assays, enzyme activity measurements and kinetics

Fermentation:

. Yeast and E. coli, downstream purification, scale up to pilot scale,

harvest/concentrate unit operations

In-Vivo Techniques:

. Rodent handling, dissection, harvesting and processing of animal

tissues, preparation and analysis of tissue sections

Microscopy:

. Immunofluorescent, scanning electron, TEM, confocal and digital

imaging

COMPUTER SKILLS

Programming Language C/C++. Visual Basic and Pascal, Visual C++.

Operating Systems Windows, UNIX/Linux, and Mac OSX

Office Software Excel, DNAStar, Word, Access, and Powerpoint

AWARDS & HONORS

. Received 2006 Merit Award from CCMB in recognition of outstanding

academic achievement.

. Best research thesis award in April 2006, by JNT University.

. Best poster presentation, June 2005 held at CCMB, International

conference held on Translational research B2B-2K5 on Cancer and other

diseases.

LEADERSHIP

. Teaching assistant at undergraduate level in Biochemistry, Cell

biology, Genetics, and Molecular Biology.

. Elected Class Representative to the Student Affairs Council.

. Representative of the Center for Cellular and Molecular Biology (CCMB)

for the Human Genome project presentation.

. Project team leader; organized and wrote presentations for scientific

meetings.

PUBLICATIONS & MEETING PRESENTATIONS

. Human Genome Diversity, poster and paper presented at a National Level

Technical Symposium, Pragnya, 2005.

. Mechanism of proteasome degradation, poster presentation at

Northwestern University, 2008

References provided upon request

Contact this candidate