KOOK-HO CHO, Ph.D.

***** ***** ***** 909-***-****

Moreno Valley, CA 92551 abk9p8@r.postjobfree.com

SUMMARY

Research Scientist with expertise in Molecular Biology, Cellular Biology, Protein Biochemistry and

Bioinformatics. Strong research, analytical, problem solving, communication and relationship

management skills.

RESEARCH SKILLS

Molecular Biology: Handling DNA & RNA, library construction (Genomic & cDNA), library screening(Phage,

E. Coli library), PCR, Real-time PCR, Vector construction, Northern, Southern blot analysis, Primer

extension, RACE, RAPD, Nucleotide Sequencing, Gene Cloning and expression, Gene tagging,

Cellular Biology: Microscopy, Confocal microscopy, In-situ hybridization, Immuno Histo Chemistry, Micro-

dissection, Insect organ culture, Cell-transfection, Drosophila cell culture, Micro-injection of mosquito,

Drosophila embryos,

Protein Biochemistry: Protein expression and purification in Prokaryotic and Eukaryotic System, SDS-

PAGE, Western blot, EMSA, GST-pull down, Immuno-precipitation, ELISA, Enzyme Immuno Assay,

Bioinformatics: Database construct and analysis (Blast 2 Go), Data base search (Fasta, Blast) Sequence

analysis (Edit Sequence, Clustal V, Gene Quencher, DNASIS, Meg Align, Vector NTI),

Laboratory Animals: Insect rearing and maintenance (Moths, Mosquitoes, Fruit flies, House flies,

Cockroach, Grass hoppers, Beetles), Rodents, Mice and Rabbit handling for experiments,

PROFESSIONAL EXPERIENCE

UNIVERSITY OF CALIFORNIA, RIVERSIDE, Riverside, CA 2002 - 2008

Assistant Specialist, Department of Biochemistry (2006 - 2008)

• Examined the epigenetic function of non-coding RNAs during the insect development.

• Investigated the function of non-coding RNAs including Tri-thorax Response Elements (TREs) and

Ubx Exons using RNA interference technology during the development of Drosophila.

Assistant Specialist, Department of Entomology, Department of Cell Biology and Neuroscience (2006)

• Searched for the effective target molecules for the control of pest insect.

• Constructed endocrine cell-specific cDNA library and screening genes for ecdysis signaling

pathways.

• Supervised graduate student: Training for the micro dissection of insect glands.

Post-Doctoral Fellow, Department of Entomology, Department of Cell Biology and Neuroscience (2003 -

2006)

• Defined the molecular mechanism underlying insect ecdysis for the developing of insect pest

control strategies.

• Elucidation of secretory mechanism for the endocrine hormone in insect ecdysis

• Effects of ecdysteroid hormone for the expression of key molecules during insect ecdysis

• Detection of different isoforms of GPCRs involved in signaling for insect ecydsis behaviour.

• Supervised undergraduate student: Training for the Immuno- Histo- Chemistry(IHC), Using con-

focal microscopy.

Post-Graduate Researcher, Department of Entomology (2002 - 2003)

• Constructed mosquito transformation vector with germ-line specific promoter to use producing

mosquitoes which can prevent the disease via ovarian transmission.

KOOK-HO CHO, Ph.D. Page 2

• Defined the regulatory region of vitellogenin receptor for germ line cell- specific expression of target

gene in mosquitoes.

MICHIGAN STATE UNIVERSITY, East Lansing, MI 1997 - 2002

Visiting Research Associate, Department of Entomology, Program in Genetics,

• Defined the expression pattern of vitellogenin Receptor (VgR) gene and gene structure to develop

non disease-transmit mosquito strains.

• Molecular analysis of vitellogenin receptor(VgR) gene in the genome of yellow fever mosquito,

Aedes aegypti

• Elucidation of expression pattern of VgR gene during mosquito vitellogenesis.

• Determined regulatory region of VgR using Drosophila transformation

• Produced antibodies against vitellogenic carboxy peptidase (part of other project)

• Trained an undergraduate student: Drosophila Micro Injection, RT-PCR

THE RESEARCH CENTER FOR NEW BIO-MATERIALS IN AGRICULTURE, Seoul National University,

Seoul, Korea 1994 - 1997

Post Doctoral Fellow (1996 - 1997)

• Cloned RIP (Ribosomal Inactivating Protein) from various vegetables for developing insect

resistance crops.

Research Associate (1994 - 1995)

EDUCATION

PhD, Molecular Biology, Seoul National University 1996

MS, Insect Physiology, Seoul National University 1990

BS, Agricultural Biology, Seoul National University 1988

PROFESSIONAL ASSOCIATIONS

Genetic Society of America: 2006 – 2008

Korean Society of Applied Entomology: 1988 – 1996

AWARDS

Korea Science and Engineering Foundation (KOSEF) Post-doctoral Fellowship,

Oct.1997 – Oct.1998

LABORATORY EQUIPMENT

Light Microscope, Electron Microscope (SEM, TEM), Con-focal Microscope, Phase Contrast Microscope,

ELISA Reader, Sequencer, PCR, Luminiometer, Fluorescence Image Analyzer, HPLC, GC,

Spectrophotometer, Embryo micro injector,

COMPUTERS

Microsoft Word, Excel, PowerPoint, Origin, Canvas, Photoshop, Gene Quencher, Vector NTI, MegAlign,

DANSIS, Edit Sequence, Clustal V…

LANGUAGES

English, Korean

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