**** ******** ***** ****, *******, FL ***** 407-***-**** (

abk4de@r.postjobfree.com

Wilfredo Seda

Objective

To obtain a meaningful and challenging position that enables me to

build on previous laboratory experience and allows for further

development of analytical skills.

Lab Coursework

Quantitative Biological Methods (Student)

Purified Glutathione-S-Transferase (GST) from an E. coli lysate using

methods such as Size Exclusion Chromatography (SEC) and Ion Exchange

Chromatography (IEX).

Determined protein concentration, reaction velocity, specific

activity, percent yield and fold purification of

Glutathione-S-Transferase (GST) and other proteins in E. coli lysate

using Bradford and DC protein assays, CDNB enzymatic assay, CB-X

protein assay and UV/Visible spectrophotometers.

Visualized Glutathione-S-Transferase (GST) on SDS-PAGE gel and Western

Blot.

Other techniques performed were ligation and transformation, miniprep

and PCR, restriction enzyme digestion and agarose gel electrophoresis.

Quantitative Biological Methods (Externship)

Research program composed of students with a high academic standing in

the BSC 3403C course, Quantitative Biological methods.

Assisted Dr. Robert Borgon in the preparation and demonstration of lab

techniques to undergraduate students.

Completed a research project signifying different ways all current lab

sections may be improved and/or more efficient.

Methods in Drug Discovery

Amplified a targeted gene of Aurora Kinase (AurK) for annealing into

ligase-free pET30 EKLIC vector and subsequently transformation into

BL21 Codonplus E. coli cells. Afterwards, the MagneHisTM Protein

Purification system was used to purify E. coli expressed proteins and

allow for determination of target protein concentration (AurK).

Determined kinetic features of target protein (AurK) such as maximum

rate of catalysis (Vmax), Michaelis constant (Km), effective dose to

inhibit 50% of biological function (IC50), and inhibition constant

(Ki) using a Luminescence-based assay for kinase activity.

Analyzed GPCR-mediated signaling and indirect activation of luciferase

gene in CRE-luc2P HEK293 cells by addition of Forskolin.

Molecular Immunology

Diagnosed the presence of bacterial and viral pathogens using the four

types of test; Agglutination, Precipitation, Enzyme Linked Immuno

Sorbent Assay, and Complement fixation.

Determined causative agents of infections including, entry, role of

pathogenicity, Immune response, and virulence.

Microbiology

Determined proper aseptic techniques on handling bacteria, collecting

cultures, and growing live specimens.

Analyzed Inoculating techniques, as well as preformed dilution ratio.

Determined appropriate selective media, proper test dependent on

pathogen, and proper Petri dish plating.

Organic Chemistry

Prepared Aspirin by acylation of salicyclic acid and acetic anhydride

with a 95.33% yield.

Performed a process known as saponification by hydrolyzing a

triglyceride and filtering it to form a crude product of soap.

Other techniques performed were aldol condensation, steam

distillation, and reflux.

Volunteer History

Florida Hospital East Orlando

March 2010 - Present

Observe Ron Sotomayor, RN in the Wound, Ostomy, and Continence

department as he diagnoses and treats patients with various

complications such as pressure ulcers, decubitus ulcers, PEG tube

infections, etc.

Facilitate the process in which Ron's orders are completed by

delivering the required materials for intervention to each patient's

room.

Allergy & Immunology Office

July 2010 - Present

Observe Dr. Santiago Martinez, MD on a weekly basis as he diagnoses

and treats patients with diseases or disorders such as asthma, upper

respiratory tract infections, allergic rhinitis, etc.

Orlando Cardiovascular Research Institute

April 2010 - Present

Assist Sally Hewitt, RN CCRC in the organization of paperwork for

clinical research trials.

Assisted with the creation of a protocol that will enable Orlando

Cardiovascular Research Institute to become an FDA approved clinic.

Florida Hospital Family Health Center

March 2009 - July 2009

Observed Dr. Brian Browning, DO deliver Osteopathic Manipulative

Therapy (OMT) to patients, with a strong emphasis on Neuromuscular

Manipulation (NMM).

Best Buy Community Services

November 2004 - Present

Participate in various yearly events such as Relay for Life (American

Cancer Society), donations to United Way, and visiting local shelters

during Thanksgiving.

Employment History

Nov 2004 - Present Inventory Specialist; Best Buy, Orlando, FL

Customer Retail

Specialist; Automotive

Technician

May 2009 - Dec 2009 Lab Teaching Assistant University of Central

Florida

Education

Aug 2006 - Dec 2009 B.S. in Molecular University of Central

Sciences & Florida, Orlando, FL

Aug 2004 - July 2006 Microbiology; B.S. in Valencia Community

Biotechnology College

A.A. in General

Studies

Skills & Qualifications

Fluent in Spanish

Extensive experience in collecting data to be used later for

analytical observation

Proficient in Microsoft Word, PowerPoint, Excel, Access, & OneNote.

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