CURRICULUM VITAE

VJEKOSLAV ZANINOVIC, M.D.

ADDRESS: *** ******* *** *** *

Kansas City KS 66101

E-MAIL: abjzm8@r.postjobfree.com

PHONE: 913-***-****

EDUCATION:

Medical Doctor (M.D.) University of Rijeka, School of Medicine, Croatia

1979-1985

RESEARCH AND MEDICAL APPOINTMENTS

CONSULTANT 2005-

2010

. GEN-PROBE, INC. 2009-

2010

Responsible for design, development and implementation of clinical

research in the field of in-vitro diagnostics. Study implementation -

Investigator Selection, Site Qualification Visits, Initiation Visits

and Interim Monitoring Visits. Protocol Development, Consent Form

Development, Clinical Regulatory Strategy Development, Monitoring Plan

Development, and Preparation of Clinical Reports. Clinical trial

oversight to ensure compliance with study protocols, IRB requirements,

FDA regulations and clinical objectives.

. TRIAGE WIRELESS, INC..

2009 Responsible for design, development and implementation of

clinical research in the field of class II medical device with

cardiovascular application. Clinical trial oversight to ensure

compliance with study protocols, IRB requirements, FDA regulations and

clinical objectives in the field of continuous noninvasive patient

monitoring.

. PHOTOTHERA, INC.

2008 Responsible for design, development and implementation of

clinical research in the field of class III medical device for the

treatment of acute ischemic stroke. Activities included Protocol

Development, Informed Consent Development and Monitoring Plan

Development. Clinical trial oversight, coordinated the activities of

CRC's/CRO to ensure compliance with study protocols, IRB requirements,

FDA regulations and clinical objectives in the field of acute ischemic

stroke.

CLINICAL RESEARCH ASSOCIATE

2001-2003

Medtronic MiniMed, Inc. Northridge, CA

Management of all facets involved in the successful conduction of clinical

trials with responsibilities including:

. Protocol development, investigators selection, site management,

and monitoring

. CRF Development, Regulatory Agency Approval and Submissions, and

interpretation of research data while ensuring ongoing

compliance of FDA guidelines and regulations

. Training of investigators and clinical site personnel on the

protocol requirements, site responsibilities and reinforcement

of GCP guidelines

. Educating and training of subjects participating in clinical

trials conducted in-house on the requirement of the protocol,

and their responsibilities during the course of the clinical

study.

Responsible for the following clinical trials:

. Continuous Glucose Monitoring System with subcutaneous and

implantable glucose sensors

. Vascular Glucose Monitoring System

. Diabetes Data Management System which provides patients and

physicians with the ability to access insulin pump and glucose meter

downloads on-line.

PROJECT SCIENTIST 1995-2001

Brentwood Biomedical Research Institution, West Los Angeles VA Medical

Center, Los Angeles, CA

. Responsible for developing study protocol, data interpretation, data

analysis using statistical methods, preparation and publication of

abstracts and papers.

. Design and conduction of experiments which elucidated early changes

that take place at the cell molecular level following tissue injury.

. Research emphasis on the activation mechanisms of the transcription

factor NF-?B and MAP kinase and their role as the regulators of pro-

inflammatory cytokines up regulation

. Experimentation with cell death models, apoptosis vs. necrosis in the

animal model and its mediation by the caspase-8 activation, TNF-? and

metalloproteinase.

. Characterization of morphological changes in inflammation by measuring

the amount and distribution of extra cellular matrix proteins by using

TGF? transgenic animals as a model.

. Studied role of adhesion molecules ICAM-1, VCAM-1 and selectins on

leukocyte-endothelium adhesion and extravasation in early phase of

inflammation.

. Using in-vivo microscopy visualized early changes in the tissue

microcirculation after caerulein infusion.

. Measured platelet aggregation in experimental animals with acute

inflammation before and after treatment with the Platelet Activating

Factor antagonist.

. Developed animal models of acute pancreatitis and pancreatic cancer in

vivo and in vitro on isolated pancreatic acinar cells.

VISITING PROFESSOR

1992-1995

University of California San Diego, San Diego, CA

Department of Gastroenterology

Project:

Role of the adhesion molecules ICAM-1, VCAM-1 and P-selectin on the

neutrophil-endothelium adhesion and neutrophil diapedesis in the cerulein

model of pancreatitis.

TEACHING ASSISTANT 1985-

1990

Department of Biochemistry

University of Rijeka, Croatia

METHODS & TECHNIQUES:

1. RT-PCR, DNA extraction and gel electrophoresis, DNA nick end labeling

TUNEL staining, immunoprecipitation, protein purification, Western Blot,

ELISA, cell and tissue culture, HPLC, tissue fixation for

immunohistochemistry, in situ hybridization, electron, confocal and

fluorescence microscopy, in vivo microscopy, use of AMBIS image analyses

system.

2. Cytokine production measurement, (TNF-(, IL-1?, IL-6, IL-10, MCP-1, MIP-

2, KC, PAF), pancreatic acinar cell isolation by collagenase digestion,

immunohistochemistry and immunofluorescence study on isolated acinar

cells and pancreatic tissue sections.

3. Developed animal (mice, rat, rabbit) models of pancreatitis, animal

surgeries, osmotic pump implantation in animals.

PUBLICATIONS:

1. Gukovskaya AS, Vaquero E, Zaninovic V, Gorelick FS, Lusis AJ, Brennan

ML, Holland S, Pandol SJ. Neutrophils and NADPH oxidase mediate

intrapancreatic trypsin activation in murine experimental pancreatitis.

Gastroenterology. 2002 Apr;122(4):974-84

2. Vaquero E, Gukovsky I, Zaninovic V, Gukovskaya AS, Pandol SJ. Localized

pancreatic NF-?B activation and inflammatory response in taurocholate-

induced pancreatitis. AM J Physiol Gastrointest Liver Physiol. 2001

Jun; 280(6):G1197-208.

3. Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ,

Gukovskaya AS. Activation of pancreatic acinar cells on isolation from

tissue: cytokine upregulation via p38 MAP kinase. AM J Physiol. 2000

Dec;279(6):C1993-2003

Zaninovic V, Gukovskaya AS, Gukovsky I, Mouria M, Pandol SJ. Cerulein up

regulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil

adhesion to these cells. Am J Physiol Gastrointest Liver Physiol. 2000

Oct;279(4):G666-76.

4. Song M, Zaninovic V, Kim D, Gukovsky I, Gukovskaya AS, Kang S, Pandol

SJ. Amelioration of cerulein-induced acute pancreatitis in the rat by

guamerin-derived synthetic peptide. Pancreas 18:231-239, 1999.

5. Gukovsky I, Gukovskaya AS, Blinman T, Zaninovic V, Pandol SJ. Early NF-

(B activation is associated with the hormone-induced pancreatitis. Am.

J. Physiol 275:G1402-G1414, 1999.

6. Periskic S, Zaninovic V, Gukovsky I, Gukovskaya AS, Solomon T, Tsukamoto

H, Pandol SJ. Ethanol feeding increases the sensitivity of the rats to

the cholecystokinin (CCK) induced pancreatitis. Gastroenterology,

1999;117:706-716

7. Gukovskaya AS, Zaninovic V, Gukovsky I, Song M, Sandoval D, Gukovsky S,

Pandol SJ. Pancreatic acinar cells produce, release and respond to tumor

necrosis factor(: Role in regulating cell death and pancreatitis. J Clin

Invest 100:1853-1862, 1997.

8. Gukovskaya AS, Perkins P, Zaninovic V, Sandoval D, Rutherford R,

Fitzsimmons TJ, Pandol SJ, Poucell-Hatton S. Mechanisms of cell death

after pancreatic duct obstruction in the opossum and the rat.

Gastroenterology 110:875-884, 1996.

9. Blinman T, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ,

Gukovskaya AS. Pancreatic acini express cytokines: up-regulation during

preparation from whole rat pancreas. Submitted to J Clin Invest.

ABSTRACTS:

1. Vaquero E, Gukovsky I, Zaninovic V, Gukovskaya AS, Pandol SJ. Up

regulation of transcription factors and cytokine mRNA expression in

taurocholate-induced pancreatitis. AGA 2000.

2. Gukovsky I, Blinman TA, Mouria M, Zaninovic V, Livington E, Pandol SJ,

Gukovskaya AS. P38 MAP kinase is a key regulator of cytokine and

chemokine expression in pancreatic acinar cells. AGA 2000.

3. Gukovskaya AS, Mouria M, Jung Y, Zaninovic V, Pandol SJ. Intracellular

mechanisms of CCK-induced apoptosis in pancreatic acinar cells. AGA

2000.

4. Gukovskaya AS, Zaninovic V, Lam H, Mouria M, Neil L, Pandol SJ.

Neutrophils regulate trypsin activation in cerulein pancreatitis.

Gastroenterology 116:A1128-A1129, 1999.

5. Gukovsky I, Jung Y, Periskic S, Zaninovic V, Kim S, Gukovskaya AS,

Tsukamoto H, Pandol SJ. Ethanol diet sensitizes pancreas to CCK-8

induced activation of NF-(B and cytokine mRNA expression.

Gastroenterology 116:A1129, 1999.

6. Zaninovic V, Gukovskaya AS, Bostandzhyan E, Eysselein VE, Pandol SJ.

Effect of over expression of transforming growth factor ( on necrotizing

cerulein pancreatitis in mice. Gastroenterology 116:A1179, 1999.

7. Blinman T, Gukovskaya AS, Gukovsky I, Zaninovic V, Livingston E, Pandol

SJ. Pancreatic acinar cells express IL-6, KC, MCP-1, and MIP-2: Evidence

from RT-PCR analysis of microscopically isolated acinar cells.

Gastroenterology 114:A443, 1998.

8. Blinman T, Gukovsky I, Gukovskaya A, Zaninovic V, Pandol SJ, Livingston

E. Intrapancreatic expression of cytokines IL-6 and KC is increased in

cerulein pancreatitis: regulation by NF-(B. Gastroenterology 114:A443,

1998.

9. Gukovskaya AS, Gukovsky I, Zaninovic V, Kira DT, Pandol SJ.

Metalloproteinases regulate signal transduction pathways triggered by

extra cellular matrix proteins. Gastroenterology 114:A464, 1998.

10. Gukovsky I, Blinman TA, Zaninovic V, Jung Y, Gukovskaya AS, Pandol SJ.

Role of transcription factor NF-(B in mediating hormone-induced

pancreatitis. Gastroenterology 114:A465, 1998.

11. Zaninovic V, Gukovskaya AS, Gukovsky I, Kim S, Pandol SJ. Adhesion

molecule ICAM-1 is present and functions in rat pancreatic acinar cells.

Gastroenterology 114:A512, 1998.

12. Song MK, Zaninovic V, Kim DR, Gukovskaya AS, Pandol SJ, Kang KW.

Peptide guamerin-D modulated development of cerulein-induced acute

pancreatitis in the rat. Gastroenterology 112:A483, 1997.

13. Periskic S, Zaninovic V, Gukovsky I, Gukovskaya AS, Solomon T,

Tsukamoto H, Pandol SJ. Ethanol feeding increases the sensitivity of the

rats to cholecystokinin (CCK) induced pancreatitis. Gastroenterology

112:A472, 1997.

14. Gukovskaya AS, Gukovsky I, Periskic S, Blinman T, Zaninovic V, Pandol

SJ. Loss of extra cellular matrix contacts leads to NF-(B activation,

cytokine expression, and production and apoptosis in pancreatic acinar

cell. Gastroenterology 112:A446, 1997.

15. Gukovsky I, Gukovskaya AS, Zaninovic V, Periskic S, Tsukamoto H, Pandol

SJ. NF-(B activation is an early marker of pancreatic acinar cell injury

in rat cerulein pancreatitis. Gastroenterology 112:A446, 1997.

16. Zaninovic V, Gukovskaya AS, Blinman T, Gukovsky I, Pandol SJ. ICAM-1

up regulation in experimental pancreatitis induced by supramaximal doses

of cerulein. Gastroenterology 112:A495, 1997.

17. Gukovskaya AS, Sandoval D, Zaninovic V, Pandol SJ. Tumor necrosis

factor regulates cell death in caerulein-induced pancreatitis.

Gastroenterology 110:A394, 1996.

18. Gukovskaya AS, Poucell-Hatton S, Perkins PS, Zaninovic V, Sandoval D,

Fitzsimmons TJ, Pandol SJ. Different mechanisms of cell death in rat and

opossum after duct ligation. Gastroenterology 108:A358, 1995.

19. Gukovskaya AS, Poucell-Hatton S, Fitzsimmons TJ, Perkins PS, Zaninovic

V, Sandoval SI, Rutherford R, Pandol, SJ. Apoptosis in experimental

pancreatitis. Gastroenterology 106:A295, 1994.

LANGUAGES:

Fluent in English, Croatian and Italian.

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