**** ***** ****** **.

N. Las Vegas, Nevada ***** Phone: 702-***-****

702-***-****

E-mail: abjzdj@r.postjobfree.com Brian P. Gibson

Objective

Presently seeking a challenging Laboratory Technician position, within a

Public or Private testing facility, where my education and multi-tasking

skill sets will be most beneficial.

Summary of Qualifications

Methodologies: Technical proficiency and theoretical application of

utilizing GC/MS, dHPLC,

Real-time PCR, Tissue Culture, DNA & RNA Extraction, as well as Rapid

high-volume Blood and serum processing and sample accessioning, all to

strict GLP & GMP compliance, respectively.

Equipment Utilized: Robotic workstations: QIAGEN BioRobot 9604 &

Beckman BIOMEK 2000; Mass Spectrometers, Thermal Cyclers, UV

Illuminators, UV PCR Cabinet/Workstations, Bio-safety and Chemical Fume

Hoods, Incubators, Centrifuges, Balances, Vortexes, Sero-Pipets &

Digital Pipettors, as well as Phase-Contrast, Fluorescent, and

Projection Microscopes, respectively.

Certifications: OSHA Bloodborne Pathogens Standard, OSHA Laboratory

Standard-Chemical Hygiene Plan, OSHA Hazard Communication Standard, WSU

OEHS Research Investigation with the Animal Model, and the U.S. NRC

Radiological Isotope Health and Safety Standard.

Relevant Laboratory Experience

Laboratory Techniques Applied:

. Maintaining quality control of laboratory testing to ensure

accurate and timely lab reporting while performing statistical

analysis & exhaustive database management.

. Conducting sample analysis of polychlorinated biphenyls (PCBs) in

groundwater, and soil, by sonification and separatory funnels

necessary for mass spectrometer analysis.

. Analyzing sample chemical matrix to determine (ppm) % utilizing

GC/MS and dHPLC.

. Consistently performing accurate calculations vital to developing

final formulations.

. Performing routine calibration and maintenance of ancillary

analytical equipment.

Research & Analysis Utilized:

. Cross-contamination free pipetting vital to performing real-time

PCR in determining polymorphisms as biomarkers for prostate and

breast carcinogenesis, respectively.

. Conducting DNA & RNA isolation, extraction, purification &

amplification for qtPCR.

. Performing Tissue culture methods in sustaining 8 cancer cell

lines, to strict protocol.

. Utilizing fluorescent microscopy necessary for image analysis of

DNA damage and repair, in determining dose response of DNA

apoptosis by chemotherapeutic drugs.

. Assisting in Gene Expression arrays essential to RNA analysis &

Assay reproducibility.

Communication Skills Developed:

. Generating clear interpretive data necessary for collaboration,

dissemination, research abstracts, and PowerPoint presentations,

as well as applications for grant support.

. Integral in drafting new protocols & SOPs to ensure conformity to

client specifications.

. Articulate testing issues and updates to colleagues, management,

and clients.

. QIAsoft 4.0, Eagle-Sight 3.2, Komet 3.1, FragmeNT analysis 5.0,

WinCyte 5.0 and SPSS.

. MS Office Suite: Excel, Word, PowerPoint, Publisher, Outlook and

Adobe Photoshop 7.0

Employment History

Laboratory Technician, Molycorp Minerals/Aerotek, Technical Operations

Dept 04/10- 08/10

Laboratory Technician, PolyOne/Manpower, Polymer Formulations Dept

06/09-12/09

Research Assistant, Wayne State University, Chemical Carcinogenesis

Dept 12/04-09/08

Laboratory Technician, the Red Cross/Maxim, Nucleic Acid Testing Dept

08/04-12/04

Laboratory Technician, Midwest Analytical Services, Organic/Semi-

Volatiles Dept 05/02-08/04

Educational History

Molecular Biology major, Analytical Chemistry minor, Wayne State

University, Senior (3.65 GPA)

Tissue Culture, ELISA, Western, Northern & Southern blotting, WSU/Inst.

Chemical Toxicology

RT-PCR & qtPCR, GS/MS, dHPLC, Molecular Epidemiology Dept., WSU/Karmanos

Cancer Inst

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