Food Medical

Uma Basavanna Ph. D

**** ***** **** ******, ********, MD 21045

Phone: 443-***-**** E-mail: abjxt6@r.postjobfree.com

RESEARCH EXPERIENCE:

Sept 2008-Present

US Food and Drug Administration, Center for Food Safety and Applied Nutrition,

Office of Regulatory Science, Division of Microbiology,College Park, MD

IRTA Postdoctoral Fellow (Advisor: Dr. Shashi Sharma)

Develop, test, optimize and validate assays for the detection and identification of

infectious disease pathogens of military interest.

REGULATORY RESEARCH: Collected and tested outbreak investigational samples as part

of FDA's regulatory effort.

Applied genomic and proteomic technologies to understand the biology of Clostridium

Botulinum, Salmonella enterica, Staphylococcal aureus species. Supervised and

managed select agent laboratory, Trained graduate students/JIFSAN trainees in

BSL-2/BSL-3 operating procedures. Documented, wrote SOP's, maintained quality

control records.

ADVANCED METHODOLOGY/PROTOCOL IMPROVEMENT: Developed and validated of novel

methodologies to identify, subtype food borne pathogens from variety of samples.

Developed a Taqman real time PCR assay for the detection of Clostridium Botulinum

serotype F neurotoxin gene. Validated high through put multiplex suspension array

system (Bioplex) in the detection and confirmation of the proteinaceous Botulinum

toxins A, B, E and F. Studied protein domain analysis and phylogenetic analysis of

BoNT neurotoxin sequences. Developed cell culture method to isolate and enrich

Salmonella enterica from shell eggs for subsequent detection by Real time PCR.

Developed Neuronal cell based FRET functional assay to detect C botulinum .

COMMUNICATION/PUBLICATION: Organized and maintained lab records, analyzed and

interpreted data, drafted research publications, presented research findings at

national and international scientific meetings.

ACCOMPLISHMENTS:

Collected and analyzed toxin producing strains of Clostridium Botulinum in Low-Acid

canned Food Plant Cooling water, Chamomile/Tea samples, Infant formula samples and

other environmental samples and reported the findings.

Developed and validated detection methods, demonstrated enhanced skill and

experience working with FRET Confocal, Fluorescence Microscopy techniques

Published the research findings in 5 peer-review journals, and one invited book

chapter.

.

Oct 2003-Oct 2006

Johns Hopkins University, Bayview Medical center, Department of Nephrology,

Baltimore, MD

Postdoctoral Fellow (Advisor: Dr. Michael Sutters)

Earliest changes in the development and Pathogenesis of Autosomal Dominant Polycystic

Kidney Disease(ADPKD)

CLINICAL STUDIES: Performed research to determine the functional aspects/role of

polycystin-1 &2, the protein products of PKD genes. Developed various cell culture

models with the conditions of over-expression and null-expression of polycystin;

studied the subsequent biological /Physiological effects. Conducted studies in animal

models.

ADVANCED INSTRUMENTATION/METHODOLOGY: Applied and optimized Intracellular/ER ca++

imaging and analysis on the Nikon Diaphot Microscopy. Demonstrated enhanced

familiarity, skill, and experience working with a variety of molecular biology

techniques, including tetracycline-inducible and adenoviral gene expression systems.

Applied strategies with somatic cell knockout and antisense technologies for

successful generation of stable cell lines with null-expression of

Polycystin-1.Prepared primary cell cultures from a heterozygous mouse line with PKD-1

conditional Knockouts as part of collaboration work.

ACCOMPLISHMENTS:

Published findings in two international peer-review journals, BBRC and Am J physic.

Presented research finding at the national and international meetings

EDUACTION:

Ph. D. Biochemistry, Mysore University, Karnataka, India.

Dissertation: "Comparative characterization of Russell's viper venom from

different regions of India"

M.Sc. Biochemistry, Mysore University, Karnataka, India.

PUBLICATIONS:

Basavanna, U, Sachdeva A, Zink D.L and Sharma, S.K (2010) Genotyping and sequencing

of dual toxin producing strains of Clostridium botulinum in Low acid canned Food

Plant cooling water. (In preparation)

Basavanna, U and Sharma SK (2010) Development of cell based flourogenic assay for the

detection of botulinum neurotoxin type A. (In preparation)

Defibaugh-Chavez S.L.H, Basavanna U and Sharma S.K (2010) A Taqman real time PCR

assay for detection of the Clostridium botulinum serotype F neurotoxin gene. (The

Botulinum Journal) Submitted

Basavanna U, Skinner GE and Sharma SK (2010). Detecting Botulinum in food: effective

and sensitive detection of Clostridium botulinum toxin in foods. In Clostridium

botulinum: A spore forming organism and a challenge to food safety: Advances in Food

Safety and Food Microbiology. Nova Science Series. Invited Chapter

Sharma S K, Basavanna U, Shukla HD. (2010) Protein Domain Analysis of C. botulinum

Type A Neurotoxin and Its Relationship with Other Botulinum Serotypes. Toxins, 2 (1),

1-9

Day JB, Basavanna U, Sharma SK. (2009) Development of a cell culture method to

isolate and enrich Salmonella enterica serotype enteritidis from shell eggs for

subsequent detection by real-time PCR. Appl Environ Microbiol. 75(16),5321-7

Weber KH, Lee EK, Basavanna U, Lindley S, Ziegelstein RC, Germino GG, Sutters M.

(2008) Heterologous expression of polycystin-1 inhibits endoplasmic reticulum

calcium leak in stably transfected MDCK cells. Am J Physio, 294(6), F1279-86.

Basavanna U, Weber KH, Hu Q, Ziegelstein RC, Germino GG and Sutters M (2006) The

isolated polycystin-1 COOH-terminal can activate or block polycystin-1 signaling.

BBRC, 359( 2), 367-372

Basavanna U and Gowda T V (2002) Molecular mechanism of lung hemorrhage induction by

VRVPLVIIIa from Russell's viper (Vipera ruselli) venom. Toxicon, 38, 1129-1147.

Prasad NB, Basavanna U, Bhat KGS and Gowda TV (2001) Comparative characterization of

Russells viper (Daboia/ Vipera russelli) venoms from different regions of India.

Biochimica et Biophysica Acta, 1428, 121-136

FELLOWSHIPS AND AWARDS:

Junior Research Fellowship, University Grants Commision (UGC) India, 1998

Senior Research Fellowship, University Grants Commission (UGC), India, 2000

Oak Ridge Institute for Science Education (ORISE) IRTA Post doctoral

Research Fellow, 2008 to present

U.S Food and Drug Administration, Center for Food Safety and Applied

Nutrition, College Park, MD ent

LABORATORY RESEARCH SKILLS:

Cell & molecular biology techniques

DNA, RNA preps; RFLP analysis; Q-RT-PCR; DNA and protein sequence analysis, Southern

and Northern analysis.

recombinant DNA construction, cloning, expression & analysis of recombinant protein,

reporter-based assays.

In vitro transcription, translation and GST pull down assays.

Immunohistochemistry & mRNA in situ analysis

Propagation,& generation of stable cell lines,constitutive and inducible

(tetracycline & tamoxifen) gene expression.

Cell based fluorescence-activated cell sorting (FACS) & enzymatic assays for antibody

mimetic biomarker screening

DNA sequencing (Sanger's method)

Protein purification and characterization

Protein purification by affinity chromatographic (glutathione affinity matrix,

conventional columns, HPLC and TLC).

Protein expression and purification in Baculovirus system.

Electrophoretic (PAGE, SDS-PAGE, IEF), spectroscopic and fluorimetric techniques.

Immunological techniques

purification and characterization of antibodies and proteins, isolation of immune

cells from peripheral blood, spleen and bone marrow, FACS analysis, ELISA-based

multiplexing (MSD's ECL, Luminex, EnVision),

potency determination of monoclonal antibodies (binding affinity, specificity,

neutralization strength), antibody neutralization and titration, immunogenicity

testing .

T cell functional assays (ADCC, CTL, ELISPOT, uptake assays, proliferation),

Determination of cytotoxic activity (LDH, Caspase3/7) cytokine assays, Western

blotting, electrophoresis (SDS-PAGE, microfluidic LabChip)

COMPUTER SKILLS:

-Operating systems: Windows and Macintosh

-DNA and protein analysis: DNA STAR, Sequencher, Mac Vector

-Data management: FileMaker Pro, multiplexing (MSD, Luminex, ) FACScan with software

Lysis II, LabChip GXII

REFERENCES:

1. Dr. Shashi K Sharma

Microbiologist

USFDA/Center for Food Science and Nutrition

5100 Paint branch parkway

College Park,MD -20780

abjxt6@r.postjobfree.com

Phone: 301-***-****

2. Dr. James B Day

Microbiologist

US FDA/Center for Food Science and Nutrition

5100 Paint branch parkway

College Park,MD -20780

abjxt6@r.postjobfree.com

Phone: 301-***-****

3. Dr. Michael Sutters

Nephrologist

Virginia Mason Medical Center

1100 Ninth Ave.

Seattle, WA 98101

abjxt6@r.postjobfree.com

Phone 206- 223-6600

Contact this candidate