La Jolla, CA, ***** Mobile: 858-***-****

Email: abjgi4@r.postjobfree.com Open to Relocation

Immigration status: Permanent Resident

OBJECTIVE: To find an opportunity that utilizes my biomedical science

knowledge and inter-personal skills towards growth of an organization and

my professional growth.

PROFILE: I am a diligent and highly motivated team player with extensive

research experience in molecular biology and biochemical techniques

including 6 years at the postdoctoral level focused on diabetes and female

reproduction using mammalian cell culture and knock out mice models. Proven

ability to execute a research project through various stages to a logical

conclusion with little or no supervision. My present position is an

Assistant Project Scientist, level II at the University of California, San

Diego.

RESEARCH AND TECHNICAL EXPERTISE

Molecular Biology/ Biochemical techniques

Mammalian cell culture, recombinant protein expression, Transfections,

Cloning, mutagenesis, RNAi (siRNA knock down, shRNA knock down), RNA-DNA

extraction, PCR, genotyping, RT-PCR, Sybr green and Taqman Real-Time PCR

(Bio-Rad, MJ Research now Biorad, Applied Biosystems, Roche),

immunofluorescence, CHIP assays, immunoprecipitation, transfections,

lentivirus transfections, Western, blot, Northern blot, Southern blot,

radioimmunoassay, EIA, ELISA, dot-blot assays,

histology/immunohistochemistry, in vitro and in vivo imaging/microscopy

(brightfield, fluorescence/confocal), primary and mammalian cell based

assays, Multi-plex/Luminex assays (LH, FSH), RNA microarray (Affymetrix,

Nimbelgen), serum/plasma analysis, flow cytometry (FACS), Insulin Tolerance

Test (ITT), IPGTT, Creating conditional (pituitary/brain) knock-out mice,

Diet-induced obesity/diabetes mouse models (inflammation, diabetes), animal

surgery, primary cell cultures, and tissue extraction/gene expression, mice

fertility assessment. Protein characterization, Post translational

modification/phosphoprotein/glycosylation analysis, Size-exclusion/gel

filtration (SEC), Ion-exchange (IEX), affinity, hydrophobic interaction

(HIC), Expanded bed chromatography (EBAC), HPLC, GC, FPLC (AKTA) and

UV/Fluorescence spectroscopy, MALDI-TOF and 2D electrophoresis.

Additionally, I have been actively training my colleagues on various

techniques from scratch.

Others Skills

? Proficient in communication (oral/written); scientific and popular

writing.

? Proficient in MS word, Excel, PowerPoint, Adobe Illustrator, Adobe

Photoshop and statistics software, JUMP IN

5.1 and Graph-pad Prism for Error analysis & ANOVA. Theoretical knowledge

of DOE/RSM.

University Service

? Lectured on '"Hormonal Regulation of Glucose and Lipid Metabolism" for a

graduate course (BIOM-266

Hormone action) in Biomedical Sciences Research program at University of

California, San Diego

? Mentored graduate/undergraduate students

Memberships

2010-present, co-Social Chair, Association of Women in Science (AWIS)

2009-present, member, Association of Women in Science (AWIS)

2009-present, member, Newsletter committee, AWIS (San Diego Chapter)

2005-present, The Endocrine Society

1996-present, Society of Biological Chemists, India

Honors and Awards

? 1998 Recipient of Certificate of Honor during MSc for scoring above

80 % marks.

? 1998 Qualified Graduate Aptitude Test for Engineering & Science:

Life Sciences with 90.59 percentile.

? 2001 Awarded Senior Research Fellowship (SRF) by Council of

Scientific and Industrial Research (CSIR) on all India level based on

appraisal of published work and interview (March, 2001 - June 2003)

External Professional Activities

? 7/2010-current: Editor, American Journal Experts

? 4/2010-current: Senior Research Proposal Consultant with H&C Scientific

Resources International, CO, USA

? 2/2010: Featured in www.voiceofSanDiego.org in the article, "When a

Doctoral Degree Isn't Enough" (Feb 8)

?2001-2003: Consultant with Prof. M.N. Gupta (IIT-Delhi) for Dabur India

limited, the fourth largest FMCG company in India (Purification of the

antidiabetic peptides of bitter gourd, Momordica charantia).

Research Accomplishments

Research articles: 25; Review articles: 2; Conferences papers: 5;

General articles: 7

Research/Work Experience

2009-present Assistant Project Scientist (II), University of

California, San Diego, Department of Medicine

? Neuronal/Brain Peroxisome Proliferator Activated Receptor ? (PPAR?)

regulates reproduction and fertility in mice.

? Diet-Induced Obesity Alters the Estrous Cyclicity in Female and

Gonadotropin Levels in Male C57BL/6 Mice.

2006-2009 Postdoctoral Fellow, University of California, San Diego,

Department of Medicine

? PPAR( Regulates the GnRH and FFA Mediated Effects on the inflammatory

Kinases and Gonadotropin Expression in Pituitary gonadotropes

? Free Fatty Acids Activate Inflammatory Kinases p38MAPK, JNK and Elevate

LH( mRNA via Toll like receptors 2/4 But Suppress FSH( mRNA In Pituitary

L(T2 Gonadotropes.

2003-2006 Postgraduate Researcher, University of California, San Diego,

Department of Rep Medicine

? Discovered that Phosphorylation of the oocyte proteins, BMP-15 and GDF-9

plays a critical role in determining agonistic or antagonistic functions.

? Characterized the posttranslational modification of recombinant human BMP-

15 mature protein by MALDI-TOF.

1998-2003 Graduate Student, Indian Institute of Technology, Delhi, India

? Developed efficient, fewer steps, high yielding protocols for

bioseparation of industrially important enzymes

including amylases, tannase, phospholipase, lipases.

? Developed reusable immobilization strategies for enzymes.

? Developed microassay format for the well known Lowry's assay for protein

estimation.

1996-1998 Master's Student, College of Basic Sciences, HPKV, Palampur,

HP, India

? Developed a simple and sensitive spectrophotometric assay for the enzyme,

tannase. The assay is still being used

by many tanning industries/workers in India and abroad.

? Purified tannase from Aspergillus niger using ion-exchange chromatography

and gel filtration.

BIBILIOGRAPHY Primary published work: Original articles

Lu M, Sarruf D, Talukdar S, Li P, Nalbandian S, Sharma S, Schwartz M, and

Olefsky JM (2010) Brain Peroxisome Proliferator-Activated Receptor Gamma is

Essential for Energy Homeostasis and the Effects of Thiazolidinediones.

Nature Letters, (under revision).

Sharma S, Sharma P, Mistry DS, Mellon P, Chang RJ, Olefsky JM, Webster NJ

(2010) PPAR( agonist Rosiglitazone suppresses activation of inflammatory

kinases and gonadotropin expression in pituitary gonadotropes. Biol Repr.,

In Press

McMahon H*, Sharma S*, Shimasaki S (2008) Phosphorylation of BMP-15 and GDF-

9 plays a critical role in determining agonistic or antagonistic functions.

Endrocrinology, 149(2):812-7. (* Equal contribution)

Saito S, Yano K, Sharma S, McMahon H, Shimasaki S. (2008) Characterization

of the posttranslational modification of recombinant human BMP-15 mature

protein. Protein Sci. 17(2):362-70.

Sugiura K1, Su Y, Pangas SA, Sharma S, Wigglesworth K, O'Brien1 MJ, Matzuk

MM, Shimasaki S, Eppig JJ (2007) Oocyte-derived BMP15 and FGFs cooperate to

promote glycolysis in cumulus cells. Development, 134 (14):2593-603.

Yoshino O, McMahon H, Sharma S, Shimasaki S. (2006) A unique preovulatory

expression pattern plays a key rolein the physiological functions of BMP-15

in the mouse. Proc Nat Acad Sci, USA 103:10678-83

Clelland E, Kohli G, Campbell RK, Sharma S, Shimasaki S, Peng C (2005) Bone

morphogenetic protein-15 in the zebrafish ovary: complementary

deoxyribonucleic acid cloning, genomic organization, tissue distribution,

and role in oocyte maturation. Endocrinology, 147, 201-209

Otsuka F, Moore RK, Wang X, Sharma S, Shimasaki S (2004) Essential role of

the oocyte in estrogen amplification of follicle-stimulating hormone

signaling in granulosa cells. Endocrinology, 146, 3362-7.

Sharma S, Gupta MN (2003) Synthesis of antioxidant propyl gallate using

tannase from Aspergillus niger van Teighem in nonaqueous media. Bioorg Med

Chem Lett 13 (3): 395-397.

Sharma S, Kaur P, Jain A, Rajeswari MR, Gupta MN (2003) A smart

bioconjugate of chymotrypsin. Biomacromolecules 4(2): 330-336.

Teotia S, Sharma S, Gupta MN (2003) Casein hydrolysis in PEG-salt two phase

system by Eudragit-chymotrypsin bioconjugate. Biotechnol. Lett. 25: 637-

639.

Shah S, Sharma A, Sharma S, Gupta MN (2003) Biodiesel Preparation by Lipase-

Catalyzed Transesterification of Jatropha Oil. Energy Fuels 18(1): 154-159.

Roy I, Sharma S, Gupta MN (2003) Smart biocatalysts: design and

applications. Adv. Biochem. Eng. Biotechnol. 86, 159-189.

Shah S, Sharma S, Gupta MN (2003) Enzymatic transesterification for

biodiesel production Indian J. Biochem. Biophys. 40, 392-399.

Sharma S, Teotia S, Gupta MN (2003) Bioconversion in aqueous two phase

system using a smart biocatalyst: Casein hydrolysis by a chymotrypsin

derivative. Enzyme Microb Technol 32: 337-339.

Sharma S, Bhat TK, Gupta MN (2002) Bioaffinity immobilization of tannase

from Aspergillus niger on concanavalin A-Sepharose CL-4B. Biotechnol Appl

Biochem 35(3): 165-169.

Roy I, Sharma S, Gupta MN (2002) Separation of an isoenzyme of polyphenol

oxidase from Duranta plumieri by expanded bed chromatography. Protein Expr

Purif 24(2): 181-187.

Jain S, Sharma S, Gupta MN (2002) A microassay for protein determination

using microwaves. Anal Biochem 311(1): 84-86.

Sharma S, Gupta MN (2001) Purification of phospholipase D from Dacus carota

by three-phase partitioning and its characterization. Protein Expr Purif

21(2): 310-316.

Sharma S, Gupta MN (2001) Alginate as a macroaffinity ligand and an

additive for enhanced activity and thermostability of lipases. Biotechnol

Appl Biochem 33:161-5.

Sharma S, Sharma A, Gupta MN (2000) One step purification of peanut

phospholipase D by precipitation with alginate. Bioseparation 9: 93-98.

Sharma A, Sharma S, Gupta MN (2000) Purification of wheat germ amylase by

precipitation. Protein Expr Purif 18: 111-114.

Sharma A, Sharma S, Gupta MN (2000) Purification of alkaline phosphatase

from chicken intestine by three-phase partitioning and use of phenyl-

Sepharose 6B in the batch mode. Bioseparation 9: 151-161.

Sharma S, Bhat TK, Dawra RK (2000) A spectrophotometric method for assay of

tannase using rhodanine. Anal Biochem 279: 85-89.

Sharma S, Bhat TK, Dawra RK (1999) Isolation, purification and properties

of tannase from Aspergillus niger van Tieghem. World J Microbiol Biotechnol

15: 673-677.

Manuscripts: Under Preparation

Sharma S, Olefsky JM, Webster NJG. Neuron-Specific PPAR( Deletion Causes

Fertility Defects in C57BL/6 Mice.

Sharma S, Webster NJG. Free Fatty acids modulate gonadotropin levels in

vitro in L(T2 cells and in vivo in C57BL/6 Mice.

Abstracts Published/Presented:

Sharma S, Sharma P, Mellon PL, Olefsky JM, Webster NJG. PPAR( agonist

rosiglitazone suppresses activation of inflammatory pathways/kinases in

pituitary L(T2 gonadotropes. SCCPIR Research meeting, May10-12, Portland,

Oregon, USA (2007) (Poster).

Sharma S, Mellon PL, Olefsky JM, Webster NJG. Free Fatty Acids Activate

Inflammatory Kinases p38MAPK And JNK and Elevate LH( mRNA But Suppress FSH(

mRNA In Pituitary L(T2 Gonadotropes. The Endocrine Society Meeting, San

Francisco, June 15-18, P2-713 (2008) (Poster)

Sharma S, Sharma P, Hedlin M, Mistry DS, Olefsky JM, Webster NJG. PPAR(

Regulates the GnRH and FFA Mediated Effects on the inflammatory Kinases and

Gonadotropin Expression in Pituitary gonadotropes. SCCPIR Research meeting,

May 10-12, Chicago, IL, USA (2009) (oral presentation)

Sharma S, Webster NJG. Diet-Induced Obesity Alters the Estrous Cyclicity in

Female and Gonadotropin Levels in Male C57BL/6 Mice. The Endocrine Society

Meeting, San Diego, June 19-22, P2-74 (2010) (Poster)

Sharma S, Olefsky JM, Webster NJG. Neuron-Specific PPAR( Deletion Causes

Fertility Defects in C57BL/6 Mice. The Endocrine Society Meeting, San

Diego, June 19-22, P1-204 (2010) (Poster)

Invited Articles, Book Chapters, General Articles

Roy I, Sharma S, Gupta MN. Smart biocatalysts: design and applications.

Adv. Biochem. Eng. Biotechnol. 86, 159-189 (2003) (Book Chapter)

Sharma S. "New Country and Career: New Lessons." The Scientist 18(7): 51

(2004) (general article)

Sharma S. "Productivity versus Hardwork" Current Science 92(8): 1031 (2007)

(general article)

Sharma S. "Meeting goals of a PhD degree" Current Science, 95(7):814 (2008)

(general article)

Sharma S. "Productivity in Science: Synergy of hard work and hard thinking"

AWIS Newsletter (2009)

Sharma S. "Careers beyond postdoctoral training" AWIS Newsletter (2009)

Sharma S. "The Power of Positive Thinking-We Are What We Think We Are" AWIS

Newsletter (Jan, 2010)

Sharma S. "Life is all about balance: Work-Life Balance" AWIS Newsletter

(March, 2010)

Sharma S. "AWIS SAN DIEGO INTRODUCES "TEAMMATES": Volunteer to mentor and

make a difference in

Youth". Newsletter (May, 2010)

EDUCATION

PhD (2003) Chemistry Department, Indian Institute of Technology

(IIT), (Biochemistry) Delhi, India

MSc (1998) College of Basic Sciences, HPKV, Palampur (Biochemistry),

(H.P.), India

BSc (1996) College of Basic Sciences, HPKV, Palampur (Basic Sciences)

(H.P.), India[pic]

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