Data Service
Resume:
STEVEN G. TERAO
**** ******** ****, *** ****, CA. 95118
*******@*****.***
OBJECTIVE
Seeking opportunities which leverage my assay automation experience to
further develop my technical skills with emphasis on lab automation,
workflow optimization, and computer technology.
AREAS OF EXPERTISE
Assay automation / Workflow Macro and template
streamlining implementation
HPLC, LC/MS In vitro ADME
Pharmacokinetics Immunoassay development
PROFESSIONAL EXPERIENCE
ROCHE PALO ALTO, Palo Alto, CA. 1996 -
2009
Research Scientist
Research Associate
Supported all early ADME efforts through experimental design; assay
automation and data interpretation. Generated data reports supporting
regulatory filings. Coordinated with various departments and outside
vendors to investigate and evaluate new technologies. Authored reports
summarizing evaluations and specifying justifications to implement new
technologies. Studied, proposed and implemented workflow improvements;
increasing overall capacity while maintaining manageable workloads. Over
12 years experience in a GLP regulated environment.
Automation
Hamilton Microlab STAR Caliper Sciclone Caliper Twister
II
Cytomat 2C Liconic STX40 Velocity 11
Vspin
Technology
Windows XP Linux
LC/MS; LC/UV
ABI Sciex Waters MicroMass Thermo Finnigan
Shimadzu HPLC Agilent HPLC Varian HPLC
LEAP Technologies HTS
PAL
In Vitro ADME
Microsome stability Hepatocyte stability Caco
permeability
CYP inhibition Protein binding
Workflow / Assay Automation
1. Studied, evaluated and automated biochemical and cell based assays,
resulting in streamlined workflows, improving assay capacity by at least
2 fold, and full time employee (FTE) utilization by at least 50%.
2. Developed, programmed, validated and implemented fully automated
microsome assay, increasing throughput 12-fold, reduced FTE 50%.
3. Developed, programmed, validated and implemented automated multispecies
hepatocyte metabolic stability assay; increased throughput 2-fold,
reduced FTE count over 50%.
4. Developed, programmed, and tested automated 384-well hepatocyte
stability assay, increased capacity 4-fold, and reduced total hepatocyte
consumption 34% enabling the potential application of hepatocytes as a
primary metabolic stability screening tool.
. Utilized operator input and system output to automatically control
workflow and enhance assay flexibility.
. Utilized templates to automatically create unique human recognizable ID
for each analyte, reducing FTE requirements 80%, and eliminated data
entry errors.
. Implemented self taught VBA programming skills, enabling development,
validation and implementation of macros, reduced FTE requirements more
than 90%, while eliminating data entry errors.
Troubleshooting / Technical Support
. Provided technical support for all automated assay technology including
system maintenance, calibration verification, troubleshoot and resolve
robot hardware/software issues, troubleshoot integrated components and
provided hardware/software upgrades and troubleshooting support for
system computer.
. Investigated all hardware, software and programming problems before
scheduling service call, minimizing down time and avoiding unnecessary
visits by application or service engineers.
. Scheduled all routine and non-routine service calls without impacting
data release timelines in over 5 years.
1. Disassembled and rewired malfunctioning LEAP autosampler avoiding 7 day
down time while service engineers were unavailable.
2. Troubleshoot and maintain Sciex and MicroMass LC/MS systems, including
routine cleaning and repair of peripheral components; minimizing down
time, and avoiding delays in data release.
Additional Contributions
. $70 - 90K savings per negotiated automation purchase through effective
communications with vendor's sales and application engineers, as well as
corporate collegues, managers and directors.
. Saved over $300K by developing and implementing ballistic LC/MS method,
reducing the total analysis time 42% and eliminating the need to
purchase an additional LC/MS system.
. Increasing FTE efficienty 100% by studing Automaton software, then
installed, validated and implemented on three LC/MS systems, enabling
automated system tuning, sample analysis and data analysis.
3. Implemented 384-well format for LC/MS analysis eliminating the need to
expand plate capacity on LC/MS systems.
. Quickly acquired and utilized working knowledge of Activity Base,
BioBook, and Documentum to document data and maintain document control.
SYNTEX CORPORATION, Palo Alto, CA. 1992 -
1996
Chemist
Provided immunoassay based analysis for preclinical PK and PK/PD studies
supporting Syntex therapeutic large molecule efforts.
1. Developed rapid thaw process preventing degradation of therapeutic
peptide, enabling sample analysis by RIA and ELISA methods.
2. Independently acquired and successfully applied cell culture techniques
to maintain monoclonal antibody cell line enabling ELISA method
development.
3. Developed and validated SPE/RIA then adapted to SPE/ELISA, enabling
outsourcing sample analysis to contract labs.
SYVA COMPANY/SYNTEX CORPORATION, San Jose, CA. 1990 -
1992
Chemist
Participated in establishing initial Lot Testing function within Systems
Support group, enabling Syva to supply customers with lot specific
performance data on each EMIT II DAU and TDM assay manufactured. Provided
customer support through Lot Testing program on DAU and TDM reagent
performance using primary analyzers.
. Trained in the use of DAU and TDM assays on all primary automated
analyzers (Hitachi 704 and 717, Olympus AU5000, Cobas Mira, Monarch
IL2000 and Synchron CX4).
. Conducted training sessions covering programming and operation of
Olympus AU5000, providing technical knowledge and hands-on operating
experience for SYVA Technical Consultants.
EDUCATION/TRAINING/AWARDS
BS Food Science, University of Washington, Seattle, WA.
"The Practice of Modern HPLC," LC Resources.
Sciex 4000, Applied Biosystems.
Winner of 2007 Roche Palo Alto DMPK Innovation Award.
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