Rochelle Holt, B.S.

**** ******* *** **** ***. **0

Orlando, FL 32817

Mobile: 941-***-****

abj2ds@r.postjobfree.com

abj2ds@r.postjobfree.com

Education University of Central Florida

Bachelor of Science in Biology (GPA: 3.5)

2010

Relevant Bio I, II & Lab Molecular Cell Biology Animal

Physiology

Coursework Chem I, II & Lab Immunology & Lab Biochemistry

Genetics Organic Chem I, II & Lab Molec.

& Cellular Pharm

Physics I & II Microbiology

Embryology/Develop

Academic Florida Bright Futures Scholar (4 years)

Honors University of Central Florida President's Honor Roll (1

semester)

University of Central Florida Deans List (3 semesters)

National SMART Grant (1 year)

Professional Intern, Metabolic Signaling & Disease Program

05/10- 11/10

Experience Sanford-Burnham Medical Research Institute

Orlando, FL

Interned in Dr. Steven Smith's laboratory: assisted with the

design and carrying out of experiments pertaining to the AMPK

and NAMPT signaling pathways as they relate to type 2 diabetes

mellitus.

Intern, Nature Works Department 02/10-

04/10

Orlando Science Center Orlando, FL

Assisted in planning an Earth Day event by contacting

participating organizations and arranging event information.

Also assisted in setting up animal and educational displays.

Skills Technical: standard lab safety, lab maintenance & inventory;

solution-making; standard cell culture technique (primary human

myotubes); adenoviral transfection; DNA, RNA & protein

extractions from cells & tissues; nucleotide quantification

(NanoDrop) & protein quantification (BCA & 660nm assays); qPCR,

qRT-PCR & Western blotting; use of 14C & 3H radioactive isotopes

for glucose uptake, glycogen synthesis & fatty acid oxidation

assays; basic mouse handling, oral gavage, intraperitoneal &

subcutaneous injections.

Academic: Experimental design, critical reading of journal

articles & oral presentation using PowerPoint.

Computer: Microsoft Word, Excel, PowerPoint & Graphpad Prism 5.

Certifications Collaborative Institutional Training Initiative (CITI):

HIPS, Good Clinical Practice,

Biomedical Research-Basic/Refresher.

Charles River: Biomethodology of the Laboratory Mouse (Technical

Skills Development).

Peer- Sheila Costford, Shantele Thomas, Meghan Gabriel,

Reviewed Rochelle Holt and Steven Smith. High palmitate exposure

Abstract results in increased NAMPT and PGC-1? expression in human

myotubes. The American College of Sports Medicine

Conference on Integrative Physiology of Exercise. 2010.

Affiliations Sanford-Burnham Science Network

05/10-Present

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