Li Li

**** **** ** **** **, Ames, IA *****

515-***-****

abihgl@r.postjobfree.com

SUMMARY

Expertise in molecular biology, anaerobic fermentation, and gas

chromatography; Experience with a range of research models,

including human, animal, plant, and bacteria; Eager to further knowledge

and skills in the biotechnology & pharmaceutical industry.

WORKING EXPERIENCE

Consultant for Kemin Health LLC, Des Moines, IA, 10/2010-present

EDUCATION & RESEARCH EXPERIENCE

Ph.D. Genetics (Minor in Toxicology), Iowa State University, Ames, IA,

08/2010

Dissertation: Assessing prebiotic effects of resistant starch with an in

vivo rat model and with an in vitro semi-continuous fermentation model

Incorporated bio-analytical and genetic tools to study the effects of gut

microbiota changes and short chain fatty acids production related to the

pre-neoplasm in rats fed resistant starches

Established a long-term in vitro fermentation system to screening

resistant starch based on their fermentability and prebiotic effects

Designed and implemented human clinical feeding trials independently to

elucidate the effects of slowly digested starches on glycemic index

(glucose and insulin)

Designed and developed forward genetic screening of mutants of

brassinosteroid signaling transduction in Arabidopsis

Developed and completed yeast one-hybrid screening to identify the

important proteins in brassinosteroid pathway

M.S. Plant Genetics and Molecular Genetics, Institute of Genetics, Chinese

Academy of Sciences, Beijing, China, 2004

Dissertation: Over-expression of nicotianamine synthase (NAS) gene improved

salt tolerance in transgenic plant models

B.S. Biotechnology, Northeast Normal University, Changchun, China, 2001

Thesis: The influence of plant hormones in Freesia tissue culture.

EXPERTISE AND SKILLS

Extensive experience in Molecular biology

DNA/RNA related techniques: plant and bacteria DNA isolation, PCR, gene

cloning, vector construction, genotyping, gene expression (Northern /

Southern blotting and RT-PCR), and PCR-DGGE

Protein related techniques: protein expression in E. coli BL21/PET1,

protein purification, yeast one-hybrid technology, electrophoretic

mobility shift assay (EMSA), GST pull down assay, ELISA, and Western

Blotting, and enzyme assays

Plant tissue culture: rice, Arabidopsis and tobacco transgenic methods,

and cross of Arabidopsis

Extensive experience in Microbiology and Bio-analysis (Gas chromatography)

Cultivation of anaerobic bacteria with anaerobic chamber (Bactron I,

Sheldon, OR)

Extraction, trace analysis and quantitative measurement of short chain

fatty acids (SCFA) during fermentation using gas chromatography

Quantitative analysis of gas production during fermentation using

Microlyzer, Quintron, WI)

Computer skills include Windows and Macintosh operative systems, and

Microsoft Office; skilled in specialized scientific software for data

processing, molecular biology analysis, graphics, statistics, and

bioanalysis, including Agilent Chemstation, Canoco, Quantity One, Adobe

Photoshop, Pair-wise alignment (BLAST), multiple sequence alignment

(ClustalX), VectorNTI

Broad knowledge with institutional review board (IRB) protocol application,

consent forms and human subjects recruiting procedures, clinical data,

including glycemic index measurement of blood glucose and insulin level,

interpretation and reporting using various statistical packages (SAS

programs and R package)

PUBLICATIONS AND PRESENTATIONS

Li Li, Heike Hofmann, Mark Campbell, Pamela White, Suzanne Hendrich.

Ruminococcus bromii related organisms was selective enriched by high

amylose starch digested residues in lean microbiota with a semi-continuous

in vitro fermentation model (manuscript to be submitted to the Journal of

Nutrition)

Li Li, Hongxin Jiang, ManYu Yum, Hyun-Jung Kim, Mark Campbell, Pamela

White, Suzanne Hendrich. Increased butyrate production by long term in

vitro fermentation of digested high amylose cornstarch residues with human

fecal microbiota (manuscript to be submitted to the Journal of Nutrition)

Li Li, Yinsheng Zhao, Jovin Hasjim, Man-Yu Yum, Diane Birt, Jane-lin Jay,

Philip Dixon, Suzanne Hendrich. Gut microbial profile is related to the

efficacy of resistant starch in decreasing aberrant crypt foci in Fisher344

rats (manuscript to be submitted to the Journal of Nutrition)

Li Li, Yum M-Y, White P, Campbell M, Hendrich S. 2010 Long-term in vitro

fermentation of high amylose starch digestion residues by human fecal

inocula, FASEB J. 24:720.2.

Zhong Ye, Erinn Rieser, Li Li and Suzanne Hendrich. Identification of

bacterial species with phenolic-degrading activity in the human oral

cavity, FASEB J. 23: 345.6, 2009

Xiaofei Yu, Li Li, Lei Li, Michelle Guo, Joanne Chory and Yanhai Yin.

Modulation of brassinosteroid-regulated gene expression by jumonji domain-

containing proteins ELF6 and REF6 in Arabidopsis, Proc Natl Acad Sci 105:

7618-7623, 2008

Sun-Ok Lee, Li Li and Suzanne Hendrich. Blood glucose response to a novel

starch, FASEB J. 22: 460.7, 2008

Li Li, Zhong Ye and Suzanne Hendrich Identification of a putative human

fecal bacterial species associated with slow gut transit time, Society of

Toxicology Central States Chapter Annual Meeting, 2007

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