Assistant Project
Resume:
Laura Millar
abhztn@r.postjobfree.com
Objective
To obtain a position in which I can gain experience as well as use my
current knowledge and skills to advance my career in science
Summary of Skills
. Knowledgeable and practiced in many common molecular biology
techniques including bead-based immunoassays; bacteria culture;
determination of cell density using spectrophotometer; DNA extraction
and purification; PCR; restriction enzyme digests; ligations;
transformations; gel electrophoresis; gel extraction; pH; DNA and
protein quantitation using UV spectrometry
. Experienced with high-throughput environments; use of various robots,
including Tecan and Beckman-Coulter liquid-handling robots
. Comfortable and experienced working both individually with very
minimal supervision as well as in a team environment where
communication is essential
. Familiar with GLP practices; detail-oriented; accurate and thorough
record-keeping
. Experienced with Microsoft Office: Word, Excel & Powerpoint
Experience
Aviir, Inc. February 2008 - current
Research Associate
. development of in-house immunoassays: conjugation of polystyrene beads
to capture antibodies, biotinylation of antibodies; testing of
potential antibody pair
. work as a team to run large serum sample sets with several different
commercial and custom multiplex bead-based assays to develop CVD
prognostic product
. operation & maintenance of Luminex/Bioplex 200 bead analyzers
. performance and occasional design of experiments to characterize and
improve various immunoassays
. analysis and presentation of data to R&D group
. creation and revision of numerous SOPs for various procedures used by
R&D
Affymetrix (Contract) April 2007 - December 2007
Research Assistant: Variation Scanning
. Work closely with a small team to construct standards for the MRD
(Mismatch Repair Detection) assay; required adhering to a timeline in
order to meet stringent deadline for project and troubleshooting of
unforeseen problems
. High-throughput primer dilution and single-plex PCR using Beckman-
Coulter Biomek FX
. Analysis of resulting PCR fragments with Caliper Life Sciences LabChip
HT
. Pool thousands of PCR products using Biomek FX and subsequent
multiplex cloning into sorter cells
. QC of DNA manipulations using SYBR Green agarose gels, and
quantitation of desired bands using ImageQuant software
. Multiplex PCR, Fragmentation, and Labeling of DNA samples
. Hybridization of DNA samples to probes on Resequencing chips
. Washing/staining and scanning of Resequencing chips using GeneChip
Fluidics stations and scanners
Amgen, Inc. December 2003 - March 2007
Associate, Research & Development: Protein Sciences
. Operated in a team environment which serves as the core DNA sequencing
resource for the research department using ABI Prism Big Dye reagents
. Responsible for batching reactions into 96-well plate format using
SWIS Workflow
. Setup and clean up of sequencing reactions using Tecan Genesis and
Genmate robots
. Operation of ABI 3100, 3700, and 3730 DNA analyzers
. Adapt to constantly increasing workload
. Organization of thousands of DNA and primer samples using Microsoft
Access
. Implemented GE Healthcare's TempliPhi technology for amplification of
plasmid DNA for sequencing; used Genetix QPIX2 automated colony picker
to pick samples for TempliPhi reactions
. Designed and performed experiments to decrease cost and increase
efficiency or test new products
. Relieved cloning group of excess workload by assisting whenever needed
. Served as back up for core plasmid prep facility - operated QIAGEN
BioRobots to prep plasmid DNA
Amgen, Inc. (Temporary) June 2003 - December 2003
Research Associate: Pharmaceutics
. Worked in a team environment to support researchers in stability
testing of potential therapeutics
. Maintained a high degree of accuracy by following precise protocols
for several different assays primarily with SDS-PAGE and subsequent
silver staining; gravimetric measurements of compounds to determine
concentration; osmometry; pH
. Compiled summary of results obtained from assays for researchers
One Lambda, Inc. (Temporary) March 2003 - June 2003
Research Associate
. Worked on individual projects to develop and improve the LABType rSSO
(reverse Sequence Specific Oligonucleotide) tissue typing test
. Conjugation of fluorescent microspheres to DNA probes
. Hybridization of conjugations to amplified DNA
. Assay with Luminex LABScan 100 flow analyzer and analyze results
. Performed various supporting duties such as machine calibration &
pipette calibration
University of California, Santa Barbara July 2001 - March 2003
Lab Assistant: Molecular, Cellular, and Developmental Biology
. Self-directed projects to further characterize the virulence factors
of Bordetella
. Primer design, PCR, cloning, plasmid rescue, Tri-parental mating
. Analysis of DNA sequence using Gene Construction Kit and Gene
Inspector
. Independently implemented gel shifts and DNase I Footprinting using
32P in order to assay the binding specificity of Bvg proteins to the
BipA gene
. ?-galactosidase assay
. Preparation of various reagents, antibiotics, gels, and media for both
E. coli and Bordetella
. Presented completed and in-progress projects as well as related
scientific papers to lab group
Publication
Fuchslocher, B., L. L. Millar, and P. A. Cotter. "Comparison of BipA
Alleles within and across Bordetella Species" Infect. Immun. June 2003.
71:3043-3052
Education
University of California, Santa Barbara
B.S. in Microbiology, June 2002
[pic]
Contact this candidate