Assistant Project

Laura Millar

********@***.***

650-***-****

Objective

To obtain a position in which I can gain experience as well as use my

current knowledge and skills to advance my career in science

Summary of Skills

. Knowledgeable and practiced in many common molecular biology

techniques including bead-based immunoassays; bacteria culture;

determination of cell density using spectrophotometer; DNA extraction

and purification; PCR; restriction enzyme digests; ligations;

transformations; gel electrophoresis; gel extraction; pH; DNA and

protein quantitation using UV spectrometry

. Experienced with high-throughput environments; use of various robots,

including Tecan and Beckman-Coulter liquid-handling robots

. Comfortable and experienced working both individually with very

minimal supervision as well as in a team environment where

communication is essential

. Familiar with GLP practices; detail-oriented; accurate and thorough

record-keeping

. Experienced with Microsoft Office: Word, Excel & Powerpoint

Experience

Aviir, Inc. February 2008 - current

Research Associate

. development of in-house immunoassays: conjugation of polystyrene beads

to capture antibodies, biotinylation of antibodies; testing of

potential antibody pair

. work as a team to run large serum sample sets with several different

commercial and custom multiplex bead-based assays to develop CVD

prognostic product

. operation & maintenance of Luminex/Bioplex 200 bead analyzers

. performance and occasional design of experiments to characterize and

improve various immunoassays

. analysis and presentation of data to R&D group

. creation and revision of numerous SOPs for various procedures used by

R&D

Affymetrix (Contract) April 2007 - December 2007

Research Assistant: Variation Scanning

. Work closely with a small team to construct standards for the MRD

(Mismatch Repair Detection) assay; required adhering to a timeline in

order to meet stringent deadline for project and troubleshooting of

unforeseen problems

. High-throughput primer dilution and single-plex PCR using Beckman-

Coulter Biomek FX

. Analysis of resulting PCR fragments with Caliper Life Sciences LabChip

HT

. Pool thousands of PCR products using Biomek FX and subsequent

multiplex cloning into sorter cells

. QC of DNA manipulations using SYBR Green agarose gels, and

quantitation of desired bands using ImageQuant software

. Multiplex PCR, Fragmentation, and Labeling of DNA samples

. Hybridization of DNA samples to probes on Resequencing chips

. Washing/staining and scanning of Resequencing chips using GeneChip

Fluidics stations and scanners

Amgen, Inc. December 2003 - March 2007

Associate, Research & Development: Protein Sciences

. Operated in a team environment which serves as the core DNA sequencing

resource for the research department using ABI Prism Big Dye reagents

. Responsible for batching reactions into 96-well plate format using

SWIS Workflow

. Setup and clean up of sequencing reactions using Tecan Genesis and

Genmate robots

. Operation of ABI 3100, 3700, and 3730 DNA analyzers

. Adapt to constantly increasing workload

. Organization of thousands of DNA and primer samples using Microsoft

Access

. Implemented GE Healthcare's TempliPhi technology for amplification of

plasmid DNA for sequencing; used Genetix QPIX2 automated colony picker

to pick samples for TempliPhi reactions

. Designed and performed experiments to decrease cost and increase

efficiency or test new products

. Relieved cloning group of excess workload by assisting whenever needed

. Served as back up for core plasmid prep facility - operated QIAGEN

BioRobots to prep plasmid DNA

Amgen, Inc. (Temporary) June 2003 - December 2003

Research Associate: Pharmaceutics

. Worked in a team environment to support researchers in stability

testing of potential therapeutics

. Maintained a high degree of accuracy by following precise protocols

for several different assays primarily with SDS-PAGE and subsequent

silver staining; gravimetric measurements of compounds to determine

concentration; osmometry; pH

. Compiled summary of results obtained from assays for researchers

One Lambda, Inc. (Temporary) March 2003 - June 2003

Research Associate

. Worked on individual projects to develop and improve the LABType rSSO

(reverse Sequence Specific Oligonucleotide) tissue typing test

. Conjugation of fluorescent microspheres to DNA probes

. Hybridization of conjugations to amplified DNA

. Assay with Luminex LABScan 100 flow analyzer and analyze results

. Performed various supporting duties such as machine calibration &

pipette calibration

University of California, Santa Barbara July 2001 - March 2003

Lab Assistant: Molecular, Cellular, and Developmental Biology

. Self-directed projects to further characterize the virulence factors

of Bordetella

. Primer design, PCR, cloning, plasmid rescue, Tri-parental mating

. Analysis of DNA sequence using Gene Construction Kit and Gene

Inspector

. Independently implemented gel shifts and DNase I Footprinting using

32P in order to assay the binding specificity of Bvg proteins to the

BipA gene

. ?-galactosidase assay

. Preparation of various reagents, antibiotics, gels, and media for both

E. coli and Bordetella

. Presented completed and in-progress projects as well as related

scientific papers to lab group

Publication

Fuchslocher, B., L. L. Millar, and P. A. Cotter. "Comparison of BipA

Alleles within and across Bordetella Species" Infect. Immun. June 2003.

71:3043-3052

Education

University of California, Santa Barbara

B.S. in Microbiology, June 2002

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