Curriculum Vitae

Mark M. Kennedy

***** ***** ******

Bristol, WI 53104

Home phone: 262-***-****

E-mail: kennedy2green @AOL.com

Summary of Qualifications

Conduct multi-disciplinary research in the areas of Animal Science,

Pharmacology, Cell and Molecular Biology utilizing in vivo, ex vivo and in

vitro experimentation.

Demonstrate a high level of responsibility in maintaining scientific

standards adhering to GLP,GMP and IACUC guidelines as well as safe

laboratory practices.

Coordinate and execute my experiments in a timely fashion and record data

efficiently and accurately. I frequently present data to colleagues and

peers.

Demonstrate creative "out of the box" thinking to solve many difficult

problems and developed new techniques to achieve project goals.

Maintain a high standard of excellence by implementation and execution of

our Quality System.

Professional Experience

Abbott Laboratories: 2000-Present

Position: R&D Tech -2005-present Polyclonal Antibody Production

*Rat Lung Assay. Product release testing of Survanta, which is used in

the treatment of Respiratory Distress Syndrome in pre-mature infants.

Lung Volume/Pressure measurements of:

VP1 Freshly excised lung

VP2 Surfactant deficient lung

VP3 Survanta instilled lung

*Abbott liaison and subject matter expert for European RLA testing at

CERB, France.

*Collection of mouse ascites for monoclonal antibody production.

*Lab Safety Officer

*Emergency Control Force member

Position: Animal Care Technician -2004-2005 Comparative Medicine

*Animal husbandry and support for neuroscience and cancer.

Position: Research Pharmacologist-2000-2004 Metabolic Disease

Pharmacology

*Drug dosing in rats and mice, Oral, IP, SC, IV

*Tail bleeds, body weights, glucose monitoring, insulin EIA

*Euthanasia and organ excision

*Small animal surgery

Baxter Healthcare Corp.: 1995-1998

Position Research Scientist Gene Therapy Unit

*Laboratory Manager of Transgenic Animal group supporting Baxter's

Gene Therapy Unit.

Abbott Laboratories: 1984-1995

Position: Research Cell Biologist 1990-1995 Aging and Degenerative

Diseases

*Culture of human embryonic kidney cells

*Culture of mouse embryonic stem cells

*Transfections of DNA into these cells

*Analysis of resultant transfectants

*Setting up and maintaining a colony of transgenic mice

*Recovery, culture and transfer of mouse embryos

*Introduction of genetic material into a mouse embryo

*Microinjection into pronuclei and blastocysts

*Breeding and analysis of transgenic mice

*Injection of ES cells into fetal placenta

*Differentiation of ES cells into various cell types

Position: Biology Research Associate 1984-1990 Hepatitis and AIDS

Research

*Culture and production of HIV stocks for assay development

*Reverse Transcriptase Assay

*S35 Met-Cys cell labeling

*Western Blot Analysis

*Radio-immunoprecipitation - SDS PAGE

*PBL isolation for HIV infectivity studies

*Primary cell culture of chimpanzee liver cells

*Virus immortalization of various cell types

The Blood Center of Southeastern Wisconsin 1982- 1984

Position: Research Technician Hemophilia Foundation

*Monoclonal antibody production

*Harvesting of spleen cells and culture of myeloma cells

*PEG fusion and selection of hybridomas

*ELISA for antibody secretion

*Cloning of positive hybridomas

*Production, collection and purification of ascities fluid

*Weekly harvesting & culture of primary umbilical endothelial cells

Education

University of Wisconsin- Milwaukee B.A.- Zoology 1982

ALAT certification 2009

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