Farhat Khan

(***** Barcan Circle

Columbia, MD **044

(Telephone: 410-***-****

(Email Address: abhjvx@r.postjobfree.com

Objective: Challenging Career as an Interdisciplinary Scientist

CORE SKILLS:

. Successful hands-on experience in operations management, planning,

workflow organization, space utilization, research, analysis, and

investigations.

. Demonstrated ability to analyze information identifies significant

factors, gather pertinent data, and develop solutions.

. An effective problem-solver with excellent organizational and time

management skills; capably liaises and manages business relationships.

. Recognized for ability to educate, train, lead, evaluate, and motivate

personnel at all levels.

STRENGTHS:

. Consistently perform critical roles in ensuring the readiness of staff

and systems to support operations and organizational objectives.

. Promote a hardworking collaborative environment with excellent team

building skills; foster enthusiasm with a focus on achieving company

goals.

. Communicate effectively orally and in writing with strong presentation

skills.

. Effectively use fiscal analysis, budgetary planning, and cost control

strengths to coordinate and administer annual operating budgets.

PROFESSIONAL WORK EXPERIENCE

March 2007 - Associate Investigator Department of Regulated

Laboratories, Division of Regulated Activities, Walter Reed Army

Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD

20910.

Administrative Experience:

. Wrote quarterly and annual progress reports, and assisted the

supervisor with managing funded projects, including review and

interpret research results, compliance with the statement of work and

budget, safety consideration, regulatory requirement, and product

development plans.

. Written animal protocols and standard operating procedures (SOP) for

laboratory equipments and biological assays.

. Contributed in writing proposals applied to the Department of Defense.

Knowledgeable about the Department of Defense Congressionally Directed

Medical Research Programs (DODCDMRP).

. Worked with administrative and scientific staff, regulatory

compliance, information management and budget personnel at Walter Reed

Army Institute of Research (WRAIR).

. Provided expert analysis, evaluation and improvement of scientific

programs and their administration.

. Supervised laboratory technicians, and summer students on the assay

development, assessing their progress, organizing meetings, presenting

research data in departmental meetings, and in conferences.

Research Experience:

. Developed pro-2-PAM as a candidate drug to treat organophosphate

toxicity.

. Developed an HPLC assay to separate pro-2-PAM and the oxime 2-PAM.

. Utilized spectroscopy to quantify the conversion of pro-2-PAM to 2-

PAM.

. Studied the kinetics of reactivation of diisopropyl fluorophosphate

inhibited acetylcholinesterase with 2-PAM and pro-2-PAM.

9/1998-3/2007 Molecular Biologist Division of Malaria Vaccine

Development, Walter Reed Army Institute of Research, 503 Robert Grant

Avenue, Silver Spring, MD 20910.

Administrative Experience:

. Contributed in writing Investigational New Drug (IND) and Batch

Production Record (BPI) for the malaria vaccine FMP10, and in wrote

grant proposals.

. Supervised laboratory technicians, students on vaccine development.

. Presented data in departmental meetings and conferences, written

several SOPs for laboratory equipments and biological assays.

Research Experience:

. Constructed plasmids for expression of C3d conjugated Plasmodium

berghei antigens for a new DNA vaccine, expressed it in mammalian

cells, and characterized the product by Western blot assay.

. Constructed plasmid vector for combination vaccine, this chimeric

protein was expressed in E. coli and purified on Ni-NTA and GST-

Sepharose affinity resin for the recombinant protein vaccine.

Antibodies were raised in mice and rabbit against the fusion protein,

antibody titers measured against individual components of the fusion

protein. Efficacy of the antibodies was measured in a growth

inhibition assay.

. Codon harmonized Plasmodium falciparum antigens were expressed in E.

coli and purified on Ni-NTA and ion-exchange chromatography for

recombinant protein vaccine.

. Constructed a plasmid vector of codon harmonized malaria antigens

without His tag, and expressed in E. coli. Effect of His-tag on the

proteins solubility, structure, and immune responses was studied.

. Estimated residual endotoxins, imidazole, sarkosyl, and E. coli

contaminant proteins in final recombinant vaccine product.

. Developed ELISA and Western blot assays using conformation specific

antibodies to determine native structure of recombinant vaccine

product.

1/1996-8/1998 Research Associate: Department of Animal sciences,

Bldg. 142. University of Maryland at College Park, MD 20742.

. Successfully cloned gene of Glucosidase 1 and characterized

promoter activity of this gene. Supervised Masters' student in

purification of glucosyltransferase.

1/1994 -12/1995 Postdoctoral Trainee: Department of Psychiatry

and Behavioral Sciences, Ross 618, 720 Rutland Avenue, Johns

Hopkins University, Baltimore, MD 21205.

. Completed cDNA cloning and in vitro transcription and

translation of the rat homolog of Dentatorubral and

pallidoluysian atrophy (DRPLA) gene. Participated in cDNA

cloning of Huntington disease gene.

3/1989- 12/1993 Research Assistant: Department of Biochemistry,

N Y U Medical Center, 550 First Avenue, New York, N Y 10016.

. Completed cDNA cloning and Sequence analysis of a human type A/B

hnRNP protein. Worked on genetic linkage mapping and comparative

mapping of mouse chromosome 12 by identifying DNA markers.

9/1986-2/1989 Postdoctoral Fellow: Department of Chemistry &

Biochemistry, University of Notre Dame, Notre Dame, IN 46556.

. Studied regulation of glycolipid glycosyltransferases in colon

carcinoma and neuroblastoma cell line.

COMPUTER INFORMATION TECHNOLOGY SKILLS

. Proficient in use of most major computer systems, information systems,

spreadsheet applications, graphic and presentation applications, and

word processing applications, including but not limited to: Windows 98

/ ME / 2000 / XP / Vista; Microsoft WordPerfect; Microsoft Office

Suite: MS Word, MS Excel, MS Access, PowerPoint; MS Publisher;

Internet Explore; Netscape Navigator.

. Plasmid construction, using Vector NTI computer program. Empower

3 software for Waters HPLC system.

EDUCATION

. 7/82-7/85 Doctor of Philosophy, Chemistry, Aligarh University,

Aligarh, U.P., India.

. 5/80-6/82 Master of Philosophy, Chemistry, Aligarh University,

Aligarh, U.P., India.

. 6/77-5/79 Master of Science, Chemistry, Aligarh University,

Aligarh, U.P., India.

. 5/74-6/77 Bachelor of Science, Chemistry, Aligarh University,

Aligarh, U.P., India.

HONORS

. Siple Award at 26th Army Science Conference 2008, Orlando,

Florida.

. Postdoctoral Trainee Fellowship, National Institute of Health,

Bethesda, MD USA. 1/1994-12/1995.

. Research Associateship, Council of Scientific & Industrial

Research, New Delhi, India, 1986.

. Senior Research Fellowship, Department of Science & Technology,

New Delhi, India, 1985-86.

. Junior & Senior Research Fellowship Council of Scientific &

Industrial Research, New Delhi, India, 1980-85

. Postgraduate Merit Scholarship for Masters Degree in Chemistry,

Aligarh University. 1977-79.

TRAININGS

2010 Regulatory Overview of Drug and Biologics Development,

Gaithersburg, MD.

2010 Basic Flow Cytometry Course, WRAIR, Silver Spring, MD

20910.

2009 Introduction to FDA Good Laboratory Practices, USAMRIID,

Frederick, MD.

2007. Lagomorphs Handling Techniques, WRAIR, Silver Spring,

MD 20910.

2007. Laboratory Animal Care Course, WRAIR, Silver Spring,

MD 20910.

2007 Rodent Handling Techniques, WRAIR Silver Spring, MD

20910.

1986-2010 Radiation Safety, University of Notre Dame, IN, NYU

Medical Center, NY, Johns

Hopkins University, Baltimore, MD and WRAIR,

Silver Spring, MD 20910.

2004. Compliance with Good Manufacturing Practices (1

Semester), UMBC, MD 21244.

2004 Good Laboratory Practices Regulation (16 h) WRAIR,

Silver Spring MD 20910.

1999 Introduction to Biostatistics Course, (36 Class hours)

WRAIR, Silver Spring, MD 20910.

RESEARCH TECHNICAL SKILLS

. Chromatographic techniques (Hydrophobic, Ion-exchange, affinity

and size exclusion) of protein purification and biochemical

Characterization, Fast Performance Liquid Chromatography (FPLC,

Water's system), High Pressure Liquid Chromatography (HPLC)

Waters system and UPLC-MS/MS Waters system.

. Analyzed linkage of enzymatic products of carbohydrate molecules

by smith degradation methods.

. Generated affinity matrix to purify enzymes and lectins,

Performed enzyme kinetics using synthetic substrates, and

inhibitors of ?-D-mannosidase,

. Performed cell culture, including primary mouse mammary gland

cells to study developmental regulation of glucosidase.

. Performed Immunological techniques: antibody production and

purification, immunoprecipitation, Western transfer, and ELISA.

. Molecular experience includes plasmid transfection by chemical,

electrophoretic, and lipid mediated methods in eukaryotic and

prokaryotic cells; Protein expression in mammalian and

bacterial cells, 10 Liter bacterial fermentation for recombinant

malaria vaccine development; microfludization for bacterial cell

lysis; estimation of endotoxin level in purified vaccine

antigens, Southern (cDNA and genomic) and Northern transfer,

cDNA and gene cloning and DNA sequencing, construction of cDNA

and genomic library, high and low stringency colony

hybridization, restriction fragment length polymorphism (RFLP)

for genetic linkage mapping, primer extension, ribonuclease

protection assay, electrophoretic mobility shift assay, PCR/real

time PCR.

. Tumor production in mice with ascites fluid, handling laboratory

animals including mice and rabbit.

OTHER SKILLS/ABILITIES AND INTERESTS

. Acquired Project Management expertise and to knowledge of: the

substantive nature of agency programs and activities; agency missions,

policies, and objectives; management principles and processes; and the

analytical and evaluative methods and techniques for assessing program

development and organizational effectiveness and efficiency. Possess

an understanding of basic budgetary and financial management

principles and techniques. Possess skill in application of fact-

finding and investigative techniques; oral and written communications;

and development of presentations and reports.

PEER REVIEWED PUBLICATIONS

1. Pro-2-PAM therapy for central and peripheral cholinesterases. DeMar JC,

Clarkson ED, Ratcliffe RH, Campbell AJ, Thangavelu SG, Herdman CA,

Leader H, Schultz SM, Marek E, Medynets MA, Ku TC, Evans SA, Khan FA,

Owens RR, Nambiar MP, and Gordon RK. (2010) Chem. Biol. Interact. 187,

191-198.

2. Evaluation of immunoglobulin purification methods and their impact on

quality and yield of antigen-specific antibodies. Bergmann-Leitner ES,

Mease RM, Duncan EH, Khan F, Waitumbi J, and Angov E. (2008) Malaria

Journal, 7: 129.

3. A critical evaluation of different methods for measuring the functional

activity of antibodies against malaria blood stage. Bergmann-Leitner

ES, Duncan EH, Muller GE, Burge JR, Khan F, Angov E., Lyon JA (2006)

Am. J Trop. Med. Hyg. 75 (3): 437-442.

4. C3d binding to the circumsporozoite protein carboxyl-terminus deviates

immunity against malaria. Bergmann-Leitner ES, Scheiblhofer S, Weiss R,

Duncan EH, Leitner WW, Chen D, Angov E, Khan F, Williams JL, Winter DB,

Thalhamer J, Lyon JA, Tsokos GC. (2005) Int Immunol., 17(3):245-55.

5. Removal of the circumsporozoite protein (CSP)

glycosylphosphatidylinositol signal sequence from a CSP DNA vaccine

enhances induction of CSP-specific TH2 type immune responses and

improves protection against malaria infection. Scheiblhofer, S., Chen

D., Weiss, R., Khan F., Mostbock S., Fegeding K., Leitner W. W.,

Thalhamer J., and Lyon J.A. (2001) Eur. J. Immunol., 31:692-698.

6. Genomic organization and promoter activity of glucosidase I gene. Khan

F.A., Varma G.M and Vijay I.K (1999) Glycobiology, 8:797-806.

7. Truncated N-terminal fragments of Huntingtin with expanded glutamine

repeats form nuclear and cytoplasmic aggregates in cell culture. Cooper

J.K, Schilling G., Peters M.F, Herring W.J, Sharp A.H, Kaminsky Z.,

Masone J, Khan F. A, Delanoy M., Borchelt D.R., Dawson V.L, Dawson

T.M., and Ross, C.A. (1998) Hum Mol Genet., 5:783-790.

8. Biosynthesis in vitro of neolactotetrosylceramide by a

galactosyltransferase from mouse T-lymphoma: purification and kinetic

studies; synthesis of neolacto and polylactosamine core. Basu M., Wang

S-A, Tang H., Khan F.A., and Basu S. (1996) Glycoconjugate J, 13:423-

432.

9. cDNA cloning and characterization of an atrophin -1(DRPLA disease gene)

related protein. Khan F. A., Margolis R.L., Love S.L., Sharp A.H., Li S-

H, and Ross, C.A. (1996) Neurobiology of Disease. 3:121-128.

10. Refinement of the DNA Marker Maps of Mouse Chromosome 12. Khan F.,

Clarke V., and D' Eustachio P. (1994) Genomics, 21:128-137.

11. Biosynthesis in vitro of SA-Lex and SA-diLex by ?1-3 fucosyltransferase

from colon carcinoma cells and embryonic brain tissues. Basu M., Hawes

J.W., Li Z., Ghosh S., Khan F.A., Zhang S., and Basu, S. (1991)

Glycobiology, 1:527-535.

12. Cloning and Sequence Analysis of a Human Type A/B hnRNP Protein. Khan

F.A., Jaiswal A. K., and Szer, W. (1991) FEBS Lett, 290:159-161.

13. Biosynthesis in vitro of Core Lacto- Series Glycosphingolipids by N-

acetylglucosaminyltransferases from Human Colon Carcinoma Colo 205.

Basu M., Khan F. A., Das K.K. & Zhang, B. (1991) Carbohydrate Res,

209:261-277.

14. Solubilized Glycosyltransferases and Biosynthesis in vitro of

Glycolipids. Basu, S., Shaper R.J., Das K.K., Daussin F., Banerjee P.,

Khan F. A., Basu M. & Suzuki, I. (1988) Biochemie, 70:1551-1563.

15. Biosynthesis in vitro of Neuronal and Non- Neuronal Gangliosides. Basu

S., Daussin F., Banerjee P., Shaper R.J., Das K.K, Khan F. A., & Basu

M. (1988) New Trends in Ganglioside Research: Neurochemical &

Neurogenerative Aspects, Plenum Book, (Ed. Leeden, R. et.al.) Livina

Press, Padova, P. 259-273.

16. Biosynthesis of Tumor Related Glycosphingolipids. Basu M., Das K.K.,

Zhang B., Khan F. A., and Basu S. (1988) Indian J. Biochem. Biophys.,

25:112-118.

17. Characterization of Lysosomal ?-D-Mannosidase from Human Placenta. Basu

D., Nair J.V. and Khan, F.A. (1988) Indian J. Biochem. Biophys. 25:695-

698.

18. Effect of Chemical Modification on Placental ?-D-Mannosidase. Khan, F.

A. & Basu D. (1984). Indian J. Biochem. Biophys, 21:203-205.

19. Isolation and Characterization of Lysosomal ?-D-Mannosidase of

Placental Tissue. Khan, F.A., & Basu D. (1982) J.BioSci. 4:133-138.

MENTORSHIPS

1. Benjamin Hoyt: (undergraduate student, Connecticut State

University, development of spectrophotometric assay for

the conversion of Pro-2-PAM to 2-PAM in the presence of

riboflavin, SEAP Program, 2009)

2. Elizabeth Marek (BS): (Technician) Department of Biochemical

Pharmacology, Division of Biochemistry, WRAIR. January 2008-

August 2009.

Isolation of membrane fraction, and HPLC, assay to measure

stability of Pro-2-PAM.

3. Marie Medynets (BS): (Technician) Department of Biochemical

Pharmacology, Division of Biochemistry, WRAIR. November 2007-

March 2009.

Kinetics of reactivation of Diisopropyl fluorophosphates

inhibited Acetylcholinesterase with oximes and oxidized pro-

oximes.

4. Sonia Thangavelu (BS): (Technician) Department of Biochemical

Pharmacology. Division of Biochemistry, WRAIR. March 2007-

December 2008.

Development of in vitro assays to characterize oximes and pro-

oximes.

5. Mark Ficco (BS): (Technician) Department of Biochemical

Pharmacology, Division of Biochemistry, WRAIR. May 2007-

December 2007.

Kinetics of reactivation of Di-isopropyl fluorophosphates

inhibited Acetylcholinesterase with oximes and oxidized pro-

oximes.

6. Chris Burge: (Undergraduate Student at College Park)

Expression, and Purification of MSP142 (FUP/CAMP) and His-tag

free MSP142 (FVO) recombinant Protein. SEAP Program 2005 and

2006).

7. Govindaraj Krishnamurthy Ph.D.: (Postdoctoral Fellow USUHS)

Protein expression of C3d and CSP-C3d conjugates in E. coli

2005.

8. Ttݫ Խ?zxpX Y 7 x Ttݫ Խ?zxpX Y

7 Jessica Ackerman: (Co-operative undergraduate student from

Penn State) Three Vector Systems, and Their Components, Used in

Producing Plasmodium falciparum Recombinant Protein 2001.

9. Nishchhal Neelaveni: (Master's student at University of

Maryland at College Park, 1996-1997, Purification and

characterization of glucosyltransferase from bovine mammary

gland).

REFERENCES

1. Dr. Richard K. Gordon, Chief

Department of Regulated Laboratories

Division of Regulated Activities

Walter Reed Army Institute of Research

Silver Spring, MD 20910.

Tel.: 301-***-****

2. Dr. Evelina Angov

Division of Malaria Vaccine Development

Walter Reed Army Institute of Research

Silver Spring, MD 20910

Tel.: 301-***-****

3. Dr. Anjali Yadava

Division of Malaria Vaccine Development

Walter Reed Army Institute of Research

Silver Spring, MD 20910

Tel. No. 301-***-****

4. Dr. Peter D'Eustachio, Professor

Department of Biochemistry, N Y U Medical Center, 550

First Avenue, New York, N Y 10016.

Tel: 212-***-****

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