Assistant University

JIANRONG WU, Ph.D.

**** ***** **** **. #***

phone: 919-***-****

Chapel Hill, NC 27514

****.***@*****.***

Experienced biophysicist with expertise in biophotonics, optics, microscopy

& spectroscopy, complex data analysis, simulation and programming looking

for R&D position.

Solid physics background

Optics & biophotonics

Molecular biophysics

Fluctuation spectroscopy

Simulation & computation

Model development

Complex data analysis

Matlab, LabVIEW, Java & C

Quick learner

Problem solving

Communication

Teamwork

Professional Experience

Research Assistant Professor, University of North Carolina, Chapel Hill, NC

Sep. 2009 - present

Postdoc research associate, University of North Carolina, Chapel Hill,

NC Sep. 2008 - Aug. 2009

. Developing new imaging techniques and image processing techniques

for fluorescence-based biosensors.

. Combining fluorescence fluctuation spectroscopy with fluorescence-

based biosensors for cell signaling studies, enabling observation

of multiple protein activities at once.

. In charge of laboratory imaging systems (TIRF, confocal, two-

photon, lasers, optical components, detectors and data acquisition

interface), training users and collaboration.

Scientist & Biophysicist, BioWarn, LLC, Pittsboro, NC

Oct. 2007 - Aug. 2008

Start-up biotech company developing semiconductor-based

biosensors

. Developed models to understand the mechanism of semiconductor

biosensors and used the model to design and optimize sensor

experiment procedures.

. Conducted biosensor experiments for government funded contract.

. Developed data analysis method and analyzed experimental data

using Igor and Matlab.

. Chosen to be one of the 7 core science team members to finish

existing contracts and obtain new contracts when the company

downsized from > 30.(company closed in 2008)

Research Assistant, Emory University, Atlanta, GA

June 2003 - Oct. 2007

Advisor: Professor Keith Berland

. Utilized two-photon fluorescence imaging and spectroscopy

techniques to study protein dynamics and interactions in living

cells.

. First theoretical research on optical excitation saturation

effects in fluorescence spectroscopy measurements. First to

demonstrate that the commonly used approach to avoid such

artifacts is not sufficient. Developed a practical method to

remove related artifacts.

. Used computation to examine and evaluate various optical

configurations and how the configurations influence spectroscopy

experimental results.

. Theoretically revised anomalous diffusion model used in

fluorescence spectroscopy measurements and clarified confusions

from the current models in the field.

. Single-handedly initiated and developed cell culture &

manipulation in the lab.

Summer Research Assistant, Emory University, Atlanta, GA

June 2000 - Sep. 2000

Advisor: Professor Keith Berland

. LabView programming for automatic data acquisition control on the

imaging system

Research Assistant, Xiamen University, China

Sep. 1997 - May. 1999

Advisor: Professor Wen-xuan Lv

. Measured the water content of nonconducting materials (oil and

paper) and the resistivity of semiconductors with microwave

perturbation outside the resonance cavity.

Teaching Experience

2002. Department of Physics, Emory University, Atlanta, GA

Teaching Associate: Undergraduate General Physics:

recitation

Teaching Assistant: Undergraduate General Physics:

laboratory

1999-2000 Department of Physics, Kent State University, Kent, OH

Teaching Assistant: Undergraduate University

Physics: laboratory

1998-1999 Department of Physics: Xiamen University, China

Teaching Assistant: Undergraduate Microwave

Experiment

Education

2000-2007 Physics, Emory University, Atlanta, GA Ph.D. in Physics

Dissertation: "Protein dynamics in living cells:

fundamental developments of fluorescence correlation

spectroscopy and investigations of protein nuclear import

mechanisms"

1993-1997 Physics, Xiamen University, China, B.S. in

Physics

Related courses:

Optics

Solid state physics

Electrodynamics

Thermal physics

Digital electronics

Analog electronics

Numerical analysis

Mathematics in physics

Non-linear algebra

Awards and Honors

2007 Travel award, Emory University

2006 Poster award 2nd place, 14th Annual Bud Suddath

Bioscience Symposium

Travel award, Emory University

1998 Xie Yu-Min Physics Scholarship, Xiamen University, China

1997 Graduated with Honor of the Year, Xiamen University,

China

Sa Ben-Dong Award (top awards at Xiamen University)

1996 Xin-Hua Scientific Award (top awards at Xiamen

University)

Professional Societies and social activities

American Society of Cell Biology - since 2010

Optical Society of America - since 2010

SPIE - since 2010

Sigma Xi, the Scientific Research Society - since 2009

Biophysical Society - since 2002

President of the physics graduate students council, Xiamen University, 1998-

1999

Publications

1. Akash Gulyani, Eric Vitriol, Richard Allen, Jianrong Wu, Dmitriy

Gremyachinskiy, Steven Lewis, Brian Dewar, Lee M Graves, Brian K Kay,

Brian Kuhlman, Tim Elston and Klaus M Hahn, 2011. "A biosensor generated

via high throughput screening quantifies cell edge Src dynamics". Nature

Chemical Biology. In press.

2. Jianrong Wu, Anita Corbett and Keith Berland, 2009. "The intracellular

mobility of nuclear import receptors and NLS cargos". Biophysical J.

96(9). 3840-3849.

3. Jianrong Wu and Keith Berland, 2008. "Propagators and Time-dependent

Diffusion Coefficients for Anomalous Diffusion in Fluorescence

Correlation Spectroscopy". Biophysical J. 95(4).2049-2052

4. Jianrong Wu and Keith Berland, 2008. "Comparing the intracellular

mobility off fluorescent proteins following in vitro expression or cell

loading with Streptolysin O". Journal of Biomedical Optics. 13(3),

031214.

5. Jianrong Wu and Keith Berland, 2007. "Fluorescence Intensity is a Poor

Predictor of Saturation Effects in Two-photon Microscopy: Artifacts in

Fluorescence Correlation Spectroscopy". Microscopy Research and

Technique. 70(8), 682-686.

6. Jianrong Wu and Keith Berland, 2006. "Wave Optics Analysis of

Observation Volumes in Two-photon Fluorescence Fluctuation Spectroscopy".

Proceedings of SPIE-The International Society for Optical Engineering,

6089 (Multiphoton Microscopy in the Biomedical Sciences VI), 60890M/1-

60890M/7.

7. Nagy, Attila, Jianrong Wu and Keith Berland, 2005. "Observation Volumes

and Gamma Factors in Two-photon Fluorescence Fluctuation Spectroscopy".

Biophysical J. 89(3), 2077-2090.

8. Nagy, Attila, Jianrong Wu and Keith Berland, 2005, "Characterizing

Observation Volumes and the Role of Excitation Saturation in One-photon

Fluorescence Fluctuation Spectroscopy". J. of Biomedical Optics,

10(4), 44015.

9. Cianci, Gianguido, Jianrong Wu and Keith Berland, 2004. "Saturation

Modified Point Spread Functions in Two-photon Microscopy". Microscopy

Research and Technique, 64(2), 135-141.

10.Jianrong Wu, Shaokuan Zheng, Zurong Ni, Fen Xiao and Wenxuan Lv, 1999.

"Study on the measurement of the resistivity of semiconductor with

perturbation methods of the TE011 cylindrical cavity". Journal of

Electronic Measurement and Instrument, 13(z):104-107

Meeting Presentations:

1. R. Allen, J. Zawistowski, Jianrong Wu, A. Gulyani, E. Vitriol, T.

Elston and K. M. Hahn, " Line Scanning and Artifact Correction at the

Cell Edge in Ratio Images". American Society of Cell Biology 49th annual

meeting, Philadelphia, PA. December 2010.

2. C. Welch, T. Samson, R. Allen, M. Berginski, Jianrong Wu, S.Gomez, T.

Elston, K. Burridge, and K.M. Hahn, "RhoG Regulates lamellipodial Rac1

Activity and Focal Adhesion Dynamics vis an Endosomal Recycling

Mechanism." American Society of Cell Biology 49th annual meeting,

Philadelphia, PA. December 2010.

3. C. Welch, T. Samson, Jianrong Wu, K. Burridge and K, Hahn, "Spatio-

Temporal Dynamics of RhoG Activation during cell Migration". American

Society of Cell Biology 49th annual meeting, San Diego, CA. December

2009.

4. Jianrong Wu, A. Corbett and K. Berland, "New Insights into Nuclear

Transport Mechanisms: Intracellular Mobility of Import Receptors and

Cargoes": Biophysical Society Meeting, Long Beach, CA, February 2008.

5. Jianrong Wu, and K. Berland, "Intracellular Dynamic of Nuclear Transport

Factors by Fluorescence Correlation Spectroscopy and Fluorescence

Imaging": Biophysical Society Meeting, Baltimore, MD, March 2007.

6. Jianrong Wu, and K. Berland, 2006, "Intracellular Dynamics of Nuclear

Transport Factors by Fluorescence Correlation Spectroscopy", 14th Annual

Bud Suddath Bioscience Symposium, Atlanta. GA, April 2006.

7. Jianrong Wu, Suzette S. Pabit and Keith M. Berland, 2006, "Investigating

Nuclear Transport Using Fluorescence Fluctuation Spectroscopy",

Biophysical Society meeting, Salt Lake City, UT, February 2006.

8. Jianrong Wu, A. Nagy and K. Berland, "Saturation in Fluorescence

Correlation Spectroscopy". Emerson Center Lectureship Award Symposium,

Atlanta, GA, May 2005.

9. Jianrong Wu, S. Pabit and K. Berland, "Mobility and Interactions of NLS

Cargoes and Import receptors Measured in Living Cells with Fluorescence

Fluctuation Spectroscopy", Biophysical Society meeting, Long Beach, CA,

February 2005.

10.Nagy, Attila, Jianrong Wu and Keith M Berland, "Photodynamics in

Fluorescence Correlation Spectroscopy", Biophysical society meeting,

Baltimore, MD, February, 2004.

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