Quality Control Maintenance

RESUME

Steven Skinner

** ***** ****

Colbert, GA 30628

Phone number: 770-***-****

Email address: **********@**********.***

EDUCATION

Athens Technical College

Athens, GA

Associate Degree in Applied Science with a major in Biotechnology

03/2007 – 06/2009

Graduated Dean’s Scholar with a cumulative GPA of 3.71

The following is a list of skills obtained through a laboratory intensive hands-on training at

Athens Technical College. These laboratory skills were learned in addition to traditional college-

level courses in Biology, Microbiology, General Chemistry, and Organic Chemistry. Laboratory

work 4 or 5 days a week and additional experience through an independent research project and

an internship were required.

The internship was approximately 80 hours and was at the Driftmier Engineering Building at

the University of Georgia. The internship included collecting data for the feasibility of turning

biomass into an environmentally-friendly and alternative fuel. Also included in the internship

was studying the procedures for making biodiesel from waste vegetable oils. The independent

research project was spent writing a protocol on preparing biodiesel.

General Laboratory Skills

Laboratory Notebook Maintenance (Good Laboratory Practices), Use of Excel Spreadsheets for

Graphing, Statistical Treatments of Data, Proficient in Microsoft Office Programs (Word, Excel,

and PowerPoint)

(Please inquire if interested in looking at the laboratory notebooks)

Chemistry/Prep Techniques

Media Preparations including Selective and Special Indicator Media, Solution Preparation, Serial

Dilutions, Molarity Calculations, Sterile Techniques, Sterile Media Preparation, Use of pH and

other Ion-selective Electrode Meters, Gravimetric and Titrimetric Methods of Analysis

Protein Techniques

Differential Centrifugation and Purification, Gel Filtration Chromatography, Purification of a

Restriction Enzyme using Ion Exchange Chromatography, Fractionation, Harvest, SDS-PAGE,

Purification of Green Fluorescent Protein (GFP) by Hydrophobic Interaction Chromatography

(HIC), Molecular Weight Determination, Bradford/Lowry Assays, Enzyme Assays including

Malate Dehydrogenase and β-Galactosidase, Immunoelectrophoresis, Western Blot, ELISA

DNA Techniques

Agarose Gel Electrophoresis for DNA separation, Analysis of Plasmids, Chromosomal DNA

isolation, Restriction Analysis, Restriction Mapping, DNA Ligation, Staining and Destaining of

DNA, Photodocumentation and Analysis of DNA Gels, Conventional Transformation of E. Coli,

Electroporation, Construction of Recombinant Plasmid DNA including Blue/White Cloning of

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DNA fragments, Polymerase Chain Reaction (PCR), Southern Blot, Preparation of a Genomic

Library

Instrumental Analysis Techniques

Flame AA (Atomic Absorption) – Digestions, Standards preparation, Sample analysis

UV-VIS – Direct use of Spectrophotometer for concentration analysis

Recognition of problems with above machinery, troubleshooting, and correction of minor issues

Cell Techniques

Growth and Management of Saintpaulia ionantha and Pisum sativum, Plant Cell Cultures,

Preparation of Physiologically Active Mitochondria and Chloroplast Isolation, Marker Enzyme

Assays, Growth and Maintenance of a Monoclonal Antibody-producing hybridoma cell line in a

CO2 incubator and Bioreactor, Sterile Cell Manipulations in a Biological Safety Cabinet

EMPLOYMENT

2010-Date Sample Preparation Technician, Analytical Support Branch, Integrated

Laboratory Systems, Athens, GA (Contractor for the Environmental

Protection Agency)

Responsibilities and duties include (but are not limited to) assisting the Extraction

Chemist in the extraction and subsequent clean up of environmental samples

(water, soil, waste, wipes, air, and tissue) for analysis by GC, GC/MS, and HPLC;

Preparing reagents and standards with proper documentation and labeling;

Preparing a weekly report of laboratory activities and documenting the amount of

time spent on each project; Calibrating and/or checking thermometers, balances,

and recording temperatures of refrigerators and water baths on a daily or as

needed basis; Recording accurate and complete data to log books, spreadsheets,

and the laboratory information management system; Maintaining laboratory in an

orderly and clean state; Inventory of solvents, laboratory supplies, and advising

the Extraction Chemist of supply needs; Cleaning and preparing glassware,

assembling and disassembling extraction apparatus and conducting equipment

maintenance; Preparing samples for inorganic parameters; Working in a team

environment with a professional and positive attitude; and other duties as needed.

2005-2006 Machinist, Hayes Lemmerz International, Gainesville, GA

Worked in a manufacturing environment where wheels for automobiles were

made. Was responsible for basic maintenance on the robots and machinery that

shaved and drilled the wheels into their final shape. Used CNC to assist the

machinery in keeping in close tolerances to the desired wheel shape and size.

Used calipers and micrometers to check the machinery’s performance of cutting

the wheels. Kept a log book to record every measurement and what was done

if something went wrong. Brought sample wheels to the laboratory at regular

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intervals to do more precise testing. Helped set up the tooling for new wheels.

2002-2004 Apprentice Pressman, Blue Ridge Paper Products, Athens, GA

Worked in the Flexo department and ran a press machine that produced mostly

milk and juice cartons. A typical 8 hour day produced about a quarter of a million

small milk cartons. Helped set up the machinery in preparation for a “make-

ready.” Corrected and tweaked minor issues in the machinery before and while it

was running. Documented what was done during the day and filled out work

orders if they were needed. Loaded fresh rolls of paper on the spindle as it was

needed. Helped the Pressman set up the printing plates. Constantly checked the

cartons (quality control) to make sure they were as good as possible while the

machinery was running and made adjustments as necessary.

20012002 Line Cook, Café International, Helen, GA

Worked in a fast-paced kitchen preparing mostly German types of foods. Was

responsible for maintaining stock inventory. Was assigned the duty of picking out

a daily special and procuring any special ingredients for the special. Worked on

the grill making reubens. Occasionally worked the cheese/salad bar that

specialized in exotic and rare cheeses. Came in early to set the kitchen up and

prepared bratwurst, knockwurst, etc. for the day. Trained other cooks to help out

on other stations.

References are available upon request.

Contact this candidate