Medical University

CURRICULUM VITAE

*** ****** ******,

Charleston, SC 29407

Tel: 843-***-****

Email: ********@*****.***

Quan Fang, M.D./Ph.D.

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Education

1. 1995-2001 Ph.D. Fudan University Medical School (former

Shanghai Medical University), Shanghai, China, 2001

2. 1988-1993 M.D. Tongji Medical University, Wuhan, China, 1993

Research Experience

. 2011- current

Volunteer researcher, Medical University of South Carolina

. 2006 - 2010

Postdoctoral Fellow, Medical University of South Carolina,

Charleston, South Carolina

. 2002 -March 2006

Postdoctoral Fellow, University of South Florida, Tampa, Florida

. 2001 - September 2002

Postdoctoral Fellow, University of Pittsburgh Cancer Institute

Research Background:

Cell biology and molecular cell biology:

1. Primary cell culture & cell line generating, mouse MEF cell line

generating, cell transfection, cell cloning and in vitro cell

function assay. Virus transduction, siRNA/shRNA generation.

2. Gene cloning and subcloning, gene expression, protein expression,

protein-protein interaction analysis, protein modification

analysis.

3. Western blot and IP, CHIP assay, RT-PCR, real-time PCR, Northern

blot.

4. Monoclonal antibody and fusion protein construction, and

purification by chromatography.

Immunological techniques:

Mouse PBMC and spleen cells isolation and cell surface marker staining

CD3, CD4, CD8, NK, CD56.

T cell function assay (cytotoxic T lymphocyte assay, BD Cytokine Bead

assay, ELISPOT assay, ELISA, Bio-Rad multiplex cytokines assay).

Complement related assay (complement activation, IgG binding assay).

Intracellular staining measured by flow cytometry or fluoroscopy.

Single chain antibody (scfv) generating and clone selection. Fusion

scfv antibodies purification.

Animal experiments:

Mouse subcutaneous tumor model, animal chemotherapy, localized tumor

radiotherapy, knockout mice clone selection and maintaining.

Publications

1. Fang Q, Tomlinson S., Enhancing antitumor radiotherapy by targeting

apoptotic cell clearance. Manuscript is in preparation.

2. Neumann CA, Fang Q, Are peroxiredoxins tumor suppressors. Curr Opin

Pharmacol. 2007 Aug;7(4):375-80

3. Naidu, K.A., Fang Q., Cheng, J-Q., Nicosia V N., Coppola, D. p53

Enhances Ascorbyl Stearate Induced G2-M Arrest of Human Ovarian

Cancer Cells. Anticancer Res. 2007 Nov-Dec;27(6B):3927-34. (co-

first author)

4. Fang Q. Naidu, K. A., Naidu K. A., Sun M., Dan H C., Nicosia V N.,

Cheng, J-Q., Coppola D. Ascorbyl Stearate Inhibits Cell

Proliferation and Survival in Human Ovarian Carcinoma Cells by

Targeting PI3K/AKT2 Pathway. Anticancer Research 2006, Feb;

26(1A):203-9

5. Hu B., Guo P., Fang Q., Tao, H.-Q., Schafer, A. C., Wang, D.-G., Su

Huang, H.-J., Gunji, Y., Nagane, M., Nishikawa, R., Alitalo, K.,

Cavenee, W. K., and Cheng, S.-Y. Angiopoietin-2 Induces Human

Glioma Invasion through the Activation of Metalloprotease-2. Proc.

Natl. Acad. Sci. USA, 100: 8904-8909, 2003. (co-first author)

6. Guo P., Fang Q., Tao H.-Q., Schafer A C., FentonB. M., Ding I., Hu

B., and Cheng,

S.-Y. Overexpression of Vascular Endothelial Growth Factor

Isoforms, VEGF121 and VEGF165, Enhances Estrogen-dependent and

Estrogen-independent Human MCF-7 Breast Tumor Growth. Cancer Res.,

63: 4684-4691, 2003.

7. Hakam A., Fang Q., Karl R., and Coppola D. Coexpression of IGF-1R

and c-Src proteins in human pancreatic ductal adenocarcinoma. Dig.

Dis. Sci., 48: 1972-1978, 2003.

8. Sekharam M., Zhao H., Sun M., Fang Q., Zhang Q., Yuan Z., Dan H.C.,

Boulware D., Cheng J.Q., and Coppola D. Insulin-like growth factor

1 receptor enhances invasion and induces resistance to apoptosis of

colon cancer cells through the Akt/Bcl-x(L) pathway. Cancer Res.,

63:7708-7716, 2003.

References:

1. Stephen Tomlinson Ph.D Professor

Department of Microbiology and Immunology,

Medical University of South Carolina

173 Ashley Avenue, Room CRI 213

Charleston, SC 29425

phone: 843-***-****

Email: ********@****.***

2. Azizul Haque, Ph.D, Associate Professor

Department of Microbiology and Immunology

Medical University of South Carolina

173 Ashley Avenue, Room CRI 206

Charleston, SC 29425

Phone: 843-***-****

Email: *****@****.***

3. Santo Nicosia, M.D. Professor, Chairman

Department of Pathology, University of South Florida

12901 Bruce B. Downs, MDC Box 11

Tampa, FL 33612

Phone: 813-***-****

email: ********@***.***.***

Research interest

Specialty: breast cancer, ovarian cancer, glioma cancer, angiogenesis,

apoptosis, cell signaling, tumor metastasis, cancer immunology

Contact this candidate