High School Assistant

Deanna A. Kulpa, Ph.D.

**** ********** ***** ~ Ann Arbor, Michigan 48103

734-***-**** ~ ********@*****.***

QUALIFICATIONS PROFILE

Molecular Biology ~ Genetics ~ Immunology ~ HIV Research ~

Retrovirology

Highly tenacious and astute professional with a Ph.D. in Human Genetics

along with extensive research experience in the fields of molecular

biology, immunology, retrovirology, and microbiology. Successfully

delineated HIV's unique capacity for evading the immune system during

postdoctoral research at the University of Michigan. Adept proficiency in

BSL3 pathogens, molecular biology techniques such as PCR, RT-PCR, Q-PCR,

RNA isolation, DNA sequencing, Western, Northern, & Southern blotting and

genetic engineering of viral vectors and plasmids. Garnered a highly

competitive Irvington Institute Fellowship from the Cancer Research

Institute. Articulate communicator with confident and engaging presentation

skills. Willing to travel and relocate. Additional expertise includes:

Virus Propagation & Production HIV Infection & Immune System Evasion

Isolation of Hematopoietic Progenitors Handling of HIV-Infected Samples

Multi Parameter Flow Cytometry

Propogation of Primary Immune Cell GMP and GLP Standards & Practices

Types

Vector Design & Engineering

EDUCATION

Ph.D. in Human Genetics, 2005 - University of Michigan, Ann Arbor, Michigan

Dissertation: "LINE-1 Ribonucleoprotein Particle Retrotransposition

Intermediates"

Bachelor of Science in Microbiology, 1997 - Michigan State University, East

Lansing, Michigan

PROFESSIONAL EXPERIENCE

University of Michigan, Department of Internal Medicine, Ann Arbor, MI

(2006 - Present)

Postdoctoral Research Fellow

Fellow of the Cancer Research Institute, New York

Examined the mechanisms of HIV immune system evasion in primary and

immortalized human macrophages and T cells. Explored antigen cross-

presentation in mouse and human dendritic cells and macrophages.

Key Achievements:

. Honored with selection for a prestigious Irvington Institute

Fellowship of the Cancer Research Institute, as one of only 11

recipients chosen from among hundreds of applicants.

. Examined the unique immune system evasion capabilities of HIV.

University of Michigan, Department of Human Genetics, Ann Arbor, MI (1999 -

2006)

Postdoctoral Research Fellow (1/2006-6/2006)

Pursued short-term postdoctoral work to complete thesis research pertaining

to the study of human retroelement LINE-1.

Key Achievements:

. Gained a deeper understanding of the mechanisms surrounding human

genome evolution.

Doctoral Student (1999-2005)

Constructed and optimized genetic and biochemical assays to characterize

the intermediate formation of the human genome retroelement LINE-1.

Key Achievements:

. Identified and elucidated novel activities associated with human LINE-

1 elements in the human genome, employing biochemical and genetic

approaches.

Deanna A. Kulpa, Ph.D.

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Desmos, Inc., San Diego, CA (1997 - 1998)

Research and Development Associate

Characterized human cell lines for production of cellular adhesion molecule

Laminin-5 for therapeutic use. Ensured compliance with GLP standards and

practices.

Chiron Technologies Center for Gene Therapy, San Diego, CA (1996 - 1997)

Pilot Manufacturing Associate

Built large-scale culture systems for the production and purification of

recombinant retroviral anti-HIV therapies and anti-cancer vaccines.

Produced and lyophilized retroviral anti-HIV therapy and anti-cancer

vaccine products for use in Phase II clinical trials. Applied GMP standards

and practices.

Aastrom Biosciences, Inc., Ann Arbor, MI (3/1996 - 8/1996)

Research and Development Scientist

Created and optimized large-scale culture conditions of primary mononuclear

cells isolated from human marrow to enrich hematopoietic progenitor cells,

which replaced depleted immune cells in support of a Phase I clinical trial

of patients receiving ablative chemotherapy.

University of Michigan, Department of Microbiology, Ann Arbor, MI (1994 -

1996)

Health Science Research Assistant II

Investigated the mutagenic potential of reverse transcriptase in the

creation of retroviral genomic DNA, leveraging the model Moloney murine

leukemia virus to understand retroviral genome evolution.

Michigan State University, Department of Energy, Plant Research Laboratory,

E. Lansing, MI (1993 - 1994)

Research Assistant

Implemented sequencing and analysis of the gene locus causing nitrogen

fixation in the alfalfa symbiont Rhizobium meliloti strain L5-30 for

potential agricultural applications.

Baylor College of Medicine, Institute for Molecular Genetics, Houston, TX

(6/1992 - 8/1992)

Summer Intern

Contributed to the scanning of exons of the human PMP22 gene to detect

causative mutations in Charcot-Marie-Tooth disease patients.

Michigan State University, Department of Energy, Plant Research Laboratory,

E. Lansing, MI (1991 - 1993)

Undergraduate Research Assistant

Leveraged a mutagenesis screen to detect the gene locus responsible for the

catabolism of a unique compound by nitrogen fixing bacterium Rhizobium

meliloti strain L5-30 for potential agricultural applications.

TEACHING EXPERIENCE

University of Michigan, Ann Arbor, MI (2006 - Present)

Undergraduate and Graduate Student Mentor (Summer 2010, Winter 2009, Fall

2008)

Developed and coached an undergraduate and two graduate students during

semester rotations while advising on independent research projects.

Laboratory Instructor (Summer 2010)

Instructed a laboratory section within a postdoctoral research training

program. Directed experiments for eight medical fellows seeking training in

laboratory techniques.

Deanna A. Kulpa, Ph.D.

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RESEARCH SKILLS

. Expert in tissue culture from creation of stable transduced immortalized

cell lines to propagation in large scale bioreactors.

. Experience with spinner flasks, cell hotels, cell factories, and

continuous perfusion bioreactors.

. Experience with harvest of viral supernatant culture medium from small

and large scale tissue culture systems, including concentration through

to lyophilized product.

. Familiar with transitioning adherent cell populations into non-adherent

populations.

. Experience with primary monocyte isolation from whole blood, with

knowledge of stimulation into lineages such as T-cells, macrophages, and

dendritic cells.

. Isolation of hematopoietic progenitors from bone marrow including

expansion and viral infection in culture.

. Familiar with processing bone marrow and cord blood for cryopreservation.

. Lentivirus and retrovirus propagation and production, including work with

replication competent HIV, HIV infected blood products, and bone marrow

in BSL3 setting.

. Experience with viruses such as adenovirus and MLV.

. Expert in numerous molecular biology techniques: Western, Northern, &

Southern blotting, plasmid and nuclear DNA isolation, RNA isolation,

protein expression and purification, PCR, RT-PCR, Q-PCR, knockdown with

shRNA and siRNA, plasmid construction and design, cloning, DNA sequencing

and mutagenesis protocols.

. Proficient in immunological techniques: multi-color flow cytometry

including operation of FACScan and FACSCanto using FACSDiva and CellQuest

systems and analysis using FloJo.

. Proficient in ELISA, confocal microscopy, antibody and protein labeling.

. Experience with protein column purification, gradient centrifugation, and

fraction collection.

. Experience working with radioactivity for metabolic labeling, probe

labeling, and DNA sequencing.

. Experience with DNA sequencing, sequence analysis, and collection and

screening of large DNA sequence databases for mutational analyses.

. Proficient in Word, Excel, PowerPoint, GraphPad Prism, Adobe Photoshop

and Illustrator, Canvas, LSM Image Browser, Sequencher, and Seqed.

AWARDS & HONORS

. Suttons Bay High School Distinguished Alumnus Award, 2008

. Irvington Institute Fellowship of the Cancer Research Institute, 2007-

2009

. Association for Women in Science Educational Foundation Pre-doctoral

Program, Amy Lutz Award, 2003

. NIH Pre-doctoral Genetics Training Grant Fellowship, 2001-2003

PUBLICATIONS

. Kulpa, D.A., Del Cid, N., Raghavan, M., and Collins, K.L. (2011) HIV Nef

Hijacks a Novel Cross-Presentation Pathway to Disrupt Antigen

Presentation. Submitted.

. Wonderlich, E.R., Kulpa, D.A., Leonard, J., Leopold, K., Norman, J.M.,

and Collins, K.L. (2011) ARF-1 Activity is Required to Recruit AP-1 to

the MHC-I Cytoplasmic Tail and Disrupt MHC-I Trafficking in HIV-1

Infected Primary T Cells. Journal of Virology in review.

. Kulpa, D.A and Collins, K.L. (2011) The Emerging Role of HLA-C in HIV

Infection. Invited Review for Immunology. Accepted.

. Doucet, A.J., Hulme, A.E., Sahinovic, E., Kulpa, D.A., Kopera, H.C.,

Moldovan, J.B., Athanikar, J.N., Hasnaoui, M., Bucheton, A., Moran, J.V.,

and Gilbert, N. (2010) Physical Characterization of LINE-1

Ribonucleoprotein Particles. PLoS Genetics 6(10): e1001150.

. Garcia-Perez, J.L., Morell, M., Scheys, J.O., Kulpa, D.A., Morell, S.,

Carter, C.C., Kim, J.K., Hammer, G.D., Collins, K.L., Andrews, P.W.,

O'Shea, K.S., Menendez, P., Moran, J.V. (2010) Epigenetic Silencing of

LINE-1 Retrotransposition Events in Human Embryonic Carcinoma Cell Lines.

Nature 466: 769-773.

Deanna A. Kulpa, Ph.D.

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. Schaefer, M.R., Williams, M., Kulpa, D.A., Blakely, P.K., and Collins,

K.L. (2008) A Novel Trafficking Signal Within the HLA-C Cytoplasmic Tail

Allows Regulated Expression Upon Differentiation of Macrophages. Journal

of Immunology 180:7804-7817.

. Kulpa, D.A. and Moran, J.V. (2006) Cis-Preferential LINE-1 Reverse

Transcriptase Activity in Ribonucleoprotein Particles. Nature Structural

and Molecular Biology 13(7): 655-660.

. Hulme, A., Kulpa, D.A., Garcia-Perez, J.L., and Moran, J.V. (2006) The

Impact of LINE-1 Retrotransposition on the Human Genome. pp. 35-55. In:

Lupski, J.R. and Stankiewicz, P. (eds.), Genomic Disorders: The Genomic

Basis of Disease. Humana Press, Totowa, NJ.

. Kulpa, D.A. and Moran, J.V. (2005) Ribonucleoprotein Particle Formation

is Necessary but not Sufficient for LINE-1 Retrotransposition. Human

Molecular Genetics 14:3237-3248.

. Kulpa, D., Topping, R., Telesnitsky, A. (1997) Determination of the Site

of First Strand Transfer During Moloney Murine Leukemia Virus Reverse

Transcription and Identification of Strand Transfer-Associated Reverse

Transcriptase Errors. EMBO Journal 16(4):856-865.

. Rossbach, S., Kulpa, D.A., Rossbach, U., de Bruijn, F.J. (1994)

Molecular and Genetic Characterization of the Rhizopine Catabolism

(mocABRC) Genes of Rhizobium meliloti L5-30. Molecular and General

Genetics 245(1):11-24.

. Rossbach, S., McSpadden, B., Kulpa, D., and de Bruijn, F.J. (1994)

Rhizopine Synthesis and Catabolism Genes for the Creation of "biased

rhizospheres" and as a Marker System to Detect (Genetically Modified)

Microorganisms in the Soil- Construction of a Minimal moc Cassette. pp

223-249. In: L. Morris, C. Grim, J.S. Angle (eds.) Biotechnology Risk

Assessment: Risk Assessment Methodologies, College Park, MD.

. Roa, B.B., Garcia, C.A., Suter, U., Kulpa, D.A., Wise, C.A., Mueller, J.,

Welcher, A.A., Snipes, G.J., Shooter, E.M., Patel, P.I., Lupski, J.R.

(1993) Charcot-Marie-Tooth Disease Type 1A. Association with a

Spontaneous Point Mutation in the PMP22 Gene. New England Journal of

Medicine 329(2): 96-101.

PRESENTATIONS

. October 2010: "Adapter Protein-1 Directs MHC-I for Cross Presentation in

Antigen Presenting Cells" 18th Annual International Cancer Immunotherapy

Symposium, New York, New York (Poster) .

. September 2009: "Adapter Protein-1 Plays a Role in MHC-I Trafficking in

Antigen Presenting Cells" 17th Annual International Cancer Immunotherapy

Symposium, New York, New York (Poster).

. September 2008: "The Effects of HIV Nef Protein on MHC-I in Antigen

Presenting Cells" 16th Annual International Cancer Immunotherapy

Symposium, New York, New York (Poster).

. April 2008: Keynote speaker at Suttons Bay High School Academic Achiever

Award Ceremony, Suttons Bay, Michigan.

. June 2005: "Reverse Transcriptase Activity in Human L1 RNPs" FASEB

Summer Research Conference on Mammalian Mobile Elements, Tucson, Arizona

(Talk).

. May 2005: "Reverse Transcriptase Activity in Human L1 RNPs" 10th Annual

Meeting of the RNA Society, Banff, Alberta, Canada (Poster).

. June 2004: "ORF1 Protein in Human Retrotransposition RNP" 9th Annual

Meeting of the RNA Society, Madison, Wisconsin (Poster).

. February 2003: "Multiple Roles for the ORF1-encoded Protein in LINE-1

Retrotransposition" Keystone Symposium on Transposition and Other Genome

Rearrangements, Santa Fe, New Mexico (Poster).

. October 2002: "Multiple Roles for the ORF1-encoded Protein in LINE-1

Retrotransposition" 52nd Annual Meeting of the American Society of Human

Genetics, Baltimore, Maryland (Talk).

. June 2002: "In Search of L1 RNP Intermediates" 7th Annual Meeting of the

RNA Society, Madison, Wisconsin (Poster).

. October 2001: "In Search of L1 Retrotransposition Intermediates" 51st

Annual Meeting of the American Society of Human Genetics, San Diego,

California (Poster).

. September 2000: "Are RNPs Retrotransposition Intermediates?" Workshop on

Site-specific Recombination and Transposition 2000, Marine Biological

Laboratory, Woods Hole, Massachusetts (Poster).

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