Michael L. Hornback
Maize, KS 67101
******@*******.***
Objective
My thirteen years of training in bacteriology and basic science
research provides the foundation required to follow and
investigate useful science. I believe I am the person for the
Microbiology Specialist position.
Summary of qualifications
10 years of independent basic science research in the field of
pathogenic microbiology
Published a peer reviewed paper and currently writing another
manuscript
Worked in BSL-3 facility during entire Ph.D. dissertation and
familiar with all BSL-3 protocols
Knowledge of most genetic, molecular biological, and biochemical
methodologies
Kept labs in compliance with chemical and radiochemical
inventories and protocols
Experience
Postdoctoral Fellow 2010
Kansas University Medical Center, Kansas City, Kansas. Indranil
Biswas, mentor
Project involves dissecting the relationship of several
transcriptional regulators on the transcription of a small
metabolite operon in Streptococcus mutans
Isolated RNA to determine transcription levels of smt operon in
various mutant backgrounds and employing in vitro transcription
assays to understand the requirements necessary for transcription
of this operon
Manuscript in preparation titled "Jha, J. K., Hornback, M. L. and
I. Biswas. Streptococcus mutans CovR relieves transcriptional
silencing by nucleoid-associated protein HLP. Manuscript in
preparation.
Postdoctoral Fellow 2007-2010
Emory University, Atlanta, GA. Charles P. Moran, mentor
Performed research on Bacillus subtilis and gene regulation of a
sporulation killing factor operon (skf) during phosphate
starvation.
Used genetic techniques using mutants and promoter fusions to
determine the complex gene regulation of this operon.
Employed biochemical techniques such as protein expression and
purification of gene regulators, electromobility shift assays,
and DNAseI protection assays.
Contact person to make sure the lab is compliance with radiation
and general lab safety protocols.
Manuscript in preparation titled "Multiple regulators control
transcription of the sporulation killing factor gene in Bacillus
subtilis."
Doctoral Student 2001-2006
East Carolina University, Greenville, NC. R. Martin Roop II,
mentor
Dissertation work involved with research to determine if DNA
repair pathways are necessary for pathogenesis of Brucella
abortus
Worked primarily in BSL-3 laboratory and intimately familiar with
BSL-3 protocols.
Performed numerous in vivo assays ranging from isolation and
infection of murine macrophages to infecting mice with test
strains as well as numerous molecular biological techniques.
Published paper in Journal of Bacteriology (Hornback, M. L. and
R. M. Roop II. 2006. The Brucella abortus xthA-1 gene product
participates in base excision repair and resistance to oxidative
killing but is not required for wild-type virulence in the mouse
model. J. Bacteriol. 188(4): 1295-1300.).
Gave over twenty oral and poster presentations at various
scientific meetings
AWards
Mary Poston Award for Outstanding Student
Presentation
Fall 2005 Meeting of the North Carolina Branch of the American
Society of Microbiology
Arnold Ravin-Muriel Rogers Fellowships Travel
Awards
Wind River Conference on Prokaryotic Biology, 2003, 2005, 2006,
and 2008
Sigma Xi Award for Outstanding Doctoral Student
Presentation
12th Annual Graduate Student Research Day. Greenville, NC. March
26, 2004.
Education
East Carolina University 2001-2006
Ph.D. from the Department of Microbiology and Immunology
Kansas State University
1996-1999
Bachelors of Science in Microbiology
Dean's Honor Role 1998
Hutchinson Community College 1991-1992 and
1994-1996
Associates of Art Degree