Michael L. Hornback

**** *. ***** **. ***. ***

Maize, KS 67101

404-***-****

abhdn2@r.postjobfree.com

Objective

My thirteen years of training in bacteriology and basic science

research provides the foundation required to follow and

investigate useful science. I believe I am the person for the

Microbiology Specialist position.

Summary of qualifications

10 years of independent basic science research in the field of

pathogenic microbiology

Published a peer reviewed paper and currently writing another

manuscript

Worked in BSL-3 facility during entire Ph.D. dissertation and

familiar with all BSL-3 protocols

Knowledge of most genetic, molecular biological, and biochemical

methodologies

Kept labs in compliance with chemical and radiochemical

inventories and protocols

Experience

Postdoctoral Fellow 2010

Kansas University Medical Center, Kansas City, Kansas. Indranil

Biswas, mentor

Project involves dissecting the relationship of several

transcriptional regulators on the transcription of a small

metabolite operon in Streptococcus mutans

Isolated RNA to determine transcription levels of smt operon in

various mutant backgrounds and employing in vitro transcription

assays to understand the requirements necessary for transcription

of this operon

Manuscript in preparation titled "Jha, J. K., Hornback, M. L. and

I. Biswas. Streptococcus mutans CovR relieves transcriptional

silencing by nucleoid-associated protein HLP. Manuscript in

preparation.

Postdoctoral Fellow 2007-2010

Emory University, Atlanta, GA. Charles P. Moran, mentor

Performed research on Bacillus subtilis and gene regulation of a

sporulation killing factor operon (skf) during phosphate

starvation.

Used genetic techniques using mutants and promoter fusions to

determine the complex gene regulation of this operon.

Employed biochemical techniques such as protein expression and

purification of gene regulators, electromobility shift assays,

and DNAseI protection assays.

Contact person to make sure the lab is compliance with radiation

and general lab safety protocols.

Manuscript in preparation titled "Multiple regulators control

transcription of the sporulation killing factor gene in Bacillus

subtilis."

Doctoral Student 2001-2006

East Carolina University, Greenville, NC. R. Martin Roop II,

mentor

Dissertation work involved with research to determine if DNA

repair pathways are necessary for pathogenesis of Brucella

abortus

Worked primarily in BSL-3 laboratory and intimately familiar with

BSL-3 protocols.

Performed numerous in vivo assays ranging from isolation and

infection of murine macrophages to infecting mice with test

strains as well as numerous molecular biological techniques.

Published paper in Journal of Bacteriology (Hornback, M. L. and

R. M. Roop II. 2006. The Brucella abortus xthA-1 gene product

participates in base excision repair and resistance to oxidative

killing but is not required for wild-type virulence in the mouse

model. J. Bacteriol. 188(4): 1295-1300.).

Gave over twenty oral and poster presentations at various

scientific meetings

AWards

Mary Poston Award for Outstanding Student

Presentation

Fall 2005 Meeting of the North Carolina Branch of the American

Society of Microbiology

Arnold Ravin-Muriel Rogers Fellowships Travel

Awards

Wind River Conference on Prokaryotic Biology, 2003, 2005, 2006,

and 2008

Sigma Xi Award for Outstanding Doctoral Student

Presentation

12th Annual Graduate Student Research Day. Greenville, NC. March

26, 2004.

Education

East Carolina University 2001-2006

Ph.D. from the Department of Microbiology and Immunology

Kansas State University

1996-1999

Bachelors of Science in Microbiology

Dean's Honor Role 1998

Hutchinson Community College 1991-1992 and

1994-1996

Associates of Art Degree

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