Assistant Manager
Resume:
[pic]
Barbara Wroblewska, Ph.D.
Associate Research Professor
********@**********.***
Home Address: ***** ******** ******
Kensington, Maryland 20895
301-***-**** (home)
301-***-**** (mobile)
Qualification Overview
. Highly qualified research scientist with more than twenty years
laboratory experience and excellent academic credentials
. Author and co-author of 50+ peer reviewed scientific publications and
over 65 scientific abstracts presented at the meetings
. Substantial experience in sophisticated laboratory techniques
including cell biology, molecular biology, cell and tissue culture and
pharmacological interactions in animal models:
1. Cell biology techniques: pharmacological measurements of
receptor interactions (dose-response effects, agonist and
antagonist plots), measurements of signal transduction responses
(changes in cAMP, cGMP, PI, intracellular Ca
radioimmunoassay, ELISA, measurements of the levels of hormones
in the brain and periphery (growth hormone, prolactin, LH-RH).
2. Molecular biology techniques: reverse transcription PCR and
molecular hybridization technologies, real time quantitative
PCR, molecular cloning, transient and stable transfections of
cDNA and subcloning of stable cell lines, in situ hybridization,
Western blotting.
3. Cell and tissue culture techniques: obtaining and maintaining
primary cell cultures (brain and spinal cord neurons,
astrocytes, vascular brain endothelial and vascular smooth
muscle cells), maintaining continuous cell lines, cellular
models of neurotoxicity
4. Animal models: PCP- and amphetamine-induced model of
schizophrenia, behavioral assessment of locomotor activity,
prepulse inhibition (PPI) determination, inflammatory and acute
pain models, carotid artery occlusion ischemic model, in vivo
models of endocrinological interactions between hypothalamus and
pituitary (lesions of different brain areas).
. Expertise in laboratory research, data collection and analysis and
project management
. Substantial experience as a laboratory manager
. Supervisor of undergraduate and graduate students for the last 15
years
. Mentor of senior thesis for undergraduate students and reviewer of PhD
thesis for graduate students
. Regular involvement in performing research for grant applications,
writing grants, editing and correcting grant applications,
collaboration with grant PI
. Lecturer for undergraduate courses at the university and graduate
courses abroad
. Member of the faculty of the Interdisciplinary Program for
Neuroscience at Georgetown University
. Reviewer for several scientific journals in the area of neuroscience,
neurochemistry, molecular pharmacology, neuronal plasticity and
psychiatry.
. Invited PhD reviewer for the international graduate schools in the
area of neuroscience.
. Invited member of the organizing committees of the scientific
international meetings in the area of neuroscience.
. Invited speaker and participant to several scientific meetings both
national and international
. Ad-hock grant reviewer for NIMH, (special panel Molecular, Cellular &
Developmental Neurobiology), grant reviewer for the National Science
Foundation and primary and secondary grant reviewer for the
Department of Defense (NETRP, studies in Parkinson's disease)
Education
1995 Additional training in molecular biology- TRAC-9, The Foundation for
Advanced Education in the Sciences, NIH, Bethesda, MD.
1979 Ph.D. (Natural Sciences), University of Warsaw, Warsaw, Poland,
(Summa cum laude) - degree certified by the National Association of
Credential Evaluation Services.
1973 M.Sci. and B.Sci. (Biology), University of Warsaw, Warsaw, Poland -
degree certified by the National Association of Credential Evaluation
Services.
Work experience
2009-present Researcher, Department of Biology, Georgetown University
. Characterize signal transduction mechanism of G-protein coupled
receptors, produce cell lines stably expressing human GRM3
. Collaboration with the Department of Pediatrics Gastroenterology -
consultation for the allergen testing in breast milk using ELISA
technology
. Consultant for the Boston based company Surface Logix Inc.
1993-2009 Associate Research Professor, Department of Biology, Georgetown
University, Washington DC
. Investigate the interactions between N-acetylaspartylglutamate (NAAG)
and metabotropic glutamate receptor (mGluR3)
. Characterize the signal transduction mechanisms of mGluR3 receptor in
cultured astrocytes and neurons
. Describe the role of mGluR3 receptor in long term depression and long
term potentiation in rats
. Molecular cloning and cellular expression of enzymes which cleave NAAG
(carboxypeptidases II and III)
. Investigate the effects of the inhibition of NAAG peptidases on pain
perception and schizophrenic behavior in rat and mice models
1990-1993 Assistant Research Professor, Department of Biology, Georgetown
University, Washington DC
. Investigate the role of arachidonic acid in signal transduction of
ionotropic glutamate receptors
. Characterize the pharmacology and signal transduction mechanism of
metabotropic glutamate receptors in cerebellar neurons
. Neuroprotective role of NAAG in cultured cortical neurons
1988-1990 Assistant Research Professor, Department of Physiology and
Biophysics, Georgetown University, Washington DC
. Characterize the vasoactive role of arachidonic acid derivatives in
rat aorta and a role of prostaglandins, hydroperoxides and other
derivatives of arachidonic acid in vasodilatory responses of blood
vessels
1986-1988 Research Associate, Department of Physiology and Biophysics,
Georgetown University, Washington DC
. Investigate the role of endothelium in vasoconstriction and
vasorelaxation of coronary arteries
. Characterize the effects of estradiol on smooth muscle cell
proliferation in blood vessels
1982-1985 Visiting Fellow, Laboratory of Neuroanatomical and
Neuropathological Sciences, NINCDS, National Institutes of
Health, Bethesda, MD
. Characterization of adrenergic receptors linked to adenylate cyclase
in cultured cerebro-vascular smooth muscle and endothelial cells
. Changes in the neurotransmitter release and glycogen metabolism and
its role in brain ischemia and brain edema
. Development and characterization of cerebromicrovascular endothelium
and smooth muscle cells in culture
1979-1982 Senior Research Assistant, Medical Research Centre, Polish
Academy of Sciences, Warsaw, Poland
. Characterization of the interaction between insulin and insulin
receptor in cerebellar capillaries and astrocytes
1974-1979 Postgraduate Fellow, Department of Neuroendocrinology,
Institute of Animal Physiology & Nutrition, Polish Academy
of Sciences, Warsaw, Poland
. Characterization of the release of prolactin in sheep with lesions of
medial basal hypothalamus
. Regulation of the release of growth hormone during pregnancy and
lactation in sheep.
. Regulation of the release of LH-RH during estrous cycle in sheep
Professional Memberships
1983-Present: Member, Society for Neuroscience
1986-Present: Member, American Association for Advancement of Sciences
(AAAS)
1993-Present: Member, International Brain Research Organization (IBRO)
1997-present: Member, Polish Neuroscience Society (PTBUN)
1979-1981: Member, Polish Teachers Association
2007-present American Association of University Professors
Awards
1974-1978 Doctoral Fellowship from Polish Academy of Sciences,
1982-1985 Fogarty Visiting Fellowship, NIH,
Invited Presentations:
Sixth International Meeting on Metabotropic Glutamate Receptors, (Invited
Speaker), Taormina, Sicily, September 2008.
International Meeting of the Polish Neuroscience Society, (Invited
Speaker), Krakow, Poland, September 2005.
Fifth International Meeting on Metabotropic Glutamate Receptors, (Invited
Speaker), Taormina, Sicily, September 2005.
1st International Symposium on N-acetylaspartate, (Invited Speaker), NIH,
Bethesda, MD, 2004.
Fourth International Meeting on Function and Regulation of Metabotropic
Glutamate Receptors (Speaker), Taormina, Sicily-Italy, September 2002.
New York State Institute for Basic Research in Developmental Disabilities,
(Seminar Speaker), Staten Island, NY, September 2000.
Department of Biology, Georgetown University, (Seminar Speaker), March 2000
Third International Meeting on Function and Regulation of Metabotropic
Glutamate Receptors (Speaker), Taormina, Sicily-Italy, September 1999.
Fourth International Congress of the Polish Neuroscience Society (Speaker),
Gdansk, Poland, September 1999.
Department of Neurophysiology, Nencki Institute of Experimental Biology
(Seminar Speaker), Warsaw, Poland, September 1999
Department of Physiology and Biophysics, Georgetown University (Seminar
Speaker), December, 1998
Department of Biology, Georgetown University (Seminar Speaker), Washington,
DC, October 1998.
National Center for Toxicological Research, U.S. Food and Drug
Administration, (Seminar Speaker), Jefferson, AR, June, 1998
Nencki Institute of Experimental Biology, Polish Academy of Sciences
(Seminar Speaker), Warsaw, Poland, October 1997.
Third International Congress of the Polish Neuroscience Society (Speaker),
Lodz, Poland, September 1997.
Second International Meeting on Function and Regulation of Metabotropic
Glutamate Receptors (Speaker), Taormina, Sicily-Italy, September 1996.
First International Congress of the Polish Neuroscience Society (Speaker),
Warsaw, Poland, September 1992.
Bibliography
Peer review publications
Wroblewska B., Wegorzewska I.N., Bzdega T., Neale J.H. Type 2
metabotropic glutamate receptor (mGluR2) fails to negatively couple to
cGMP in stably transfected cells. Neurochem Int. (Epub ahead of print)
2010.
Wroblewska B. and Lewis D. Validating Novel Targets for Pharmacological
Interventions in Schizophrenia (Editorial), Am. J. Psychiatry, 2009.
Yamamoto T., Saito O., Bartlozzi A., Sarva J., Kozikowski A., Wroblewska
B., Bzdega T., Neale J.H. Local administration of N-
acetylaspartylglutamate (NAAG) peptidase inhibitors is analgesic in
peripheral pain. Eur. J. Neurosci.25:147-158, 2007.
Wroblewska B. NAAG as a neurotransmitter. Adv. Exp. Med. Biol. 576: 317-
325, proceedings from 1st International Symposium on N-acetylaspartate,
2006.
Wroblewska B., Wegorzewska I. M., Bzdega T., Olszewski R. T., Neale J. H.
Differential negative coupling of type 3 metabotropic glutamate receptor
to cyclic GMP levels in neurons and astrocytes. J. Neurochem. 96: 1071-
1077, 2006.
Neale J. H., Olszewski R. T., Gehl L. M., Wroblewska B., Bzdega T. The
neurotransmitter N-acetylaspartylglutamate in models of pain, ALS,
diabetic neuropathy, CNS injury and schizophrenia. Trends Pharmacol. Sci.
26: 477-484, 2005.
Poschel B., Wroblewska B., Heinemann U., Manahan-Vaughan D. The
metabotropic glutamate receptor mGluR3 is critically required for
hippocampal long-term depression and modulates long-term potentiation in
the dentate gyrus in freely moving rats. Cereb. Cortex 15: 1414-1423,
2005.
Yamamoto T., Hirasawa S., Wroblewska B., Grajkowska E., Zhou J.,
Kozikowski A., Wroblewski J., and Neale J. H. Antinociceptive effects of
N-acetylaspartylglutamate (NAAG) peptidase inhibitors ZJ-11, ZJ-17 and ZJ-
43 in the rat formalin test and in the rat neuropathic pain model. Eur.
J. Neurosci. 20: 483-494, 2004.
Gehl L. M., Saab O. H., Bzdega T., Wroblewska B., Neale J. H.
Biosynthesis of NAAG by an enzyme-mediated process in rat central nervous
system neurons and glia. J. Neurochem. 90: 989-997, 2004.
Kozikowski A.P., Zhang J., Petukhov P.A., Grajkowska E., Wroblewski J.T.,
Yamamoto T., Bzdega T., Wroblewska B., Neale J.H. Synthesis of urea-based
inhibitors as active site probes of glutamate carboxypeptidase II:
efficacy as analgesic agents. J. Med. Chem. 47: 1729-1738, 2004.
Bzdega T., Crowe S.L., Ramadan E.R., Sciarretta K.H., Olszewski R.T.,
Ojeifo O.A., Rafalski V.A., Wroblewska B., Neale J.H. The cloning and
characterization of a second brain enzyme with NAAG peptidase activity.
J. Neurochem 89: 627-635, 2004.
Olszewski R.T., Bukhari N., Zhou J., Kozikowski A.P., Wroblewski J.T.,
Shamimi-Norri S., Wroblewska B., Bzdega T., Vicini S., Barton F.B., Neale
J. H. NAAG peptidase inhibition reduces locomotor activity and some
stereotypes in the PCP model of schizophrenia via group II mGluR. J.
Neurochem. 89: 876-885, 2004.
Bacich D.J., Ramadan E., O'Keefe D.S., Bukhari N., Wegorzewska I., Ojeifo
O., Olszewski R., Wrenn C.C., Bzdega T., Wroblewska B., Heston W.D.,
Neale J.H. Deletion of the glutamate carboxypeptidase II gene in mice
reveals a second enzyme activity that hydrolyzes N-
acetylaspartylglutamate. J. Neurochem 83: 20-29, 2002.
Kozikowski A.P., Nan F., Conti P., Zhang J., Ramadan E., Bzdega T.,
Wroblewska B., Neale J.H., Pshenichkin S., Wroblewski J.T. Design of
remarkably simple, yet potent urea-based inhibitors of glutamate
carboxypeptidase II (NAALADase). J. Med. Chem. 44: 298-301, 2001.
Lea P.M. 4th, Wroblewska B., Sarvey J.M., Neale J.H. ? NAAG rescues LTP
from blockade by NAAG in rat dentate gyrus via the type 3 metabotropic
glutamate receptor. J. Neurophysiol. 85: 1097-1106, 2001.
Zhao J., Ramadan E., Capiello M., Wroblewska B., Bzdega T., Neale J.H.
NAAG inhibits KCl-induced 3[H]-GABA release via mGluR3, camp, PKA and L-
type calcium conductance. Eur. J. Neurosci. 13: 340-346, 2001.
Neale J. H., Bzdega T. and Wroblewska B. N-acetylaspartylglutamate: the
most abundant peptide neurotransmitter in the mammalian central nervous
system. J. Neurochemistry. 75: 443-452, 2000.
Nan F., Bzdega T., Pshenichkin S., Wroblewski J.T., Wroblewska B., Neale
J. H., Kozikowski A. P. Dual function glutamate-related ligands:
discovery of a novel, potent inhibitor of glutamate carboxypeptidase II
possessing mGluR3 agonist activity. J. Medicinal Chemistry. 43:772-774,
2000.
Bruno V., Wroblewska B., Wroblewski J. T. and Nicoletti F.
Neuroprotective activity of N-acetylaspartylglutamate in cultured
cortical cells. Neuroscience 85: 751-757, 1998.
Wroblewska B., Santi M. R. and Neale J. H. N-acetylaspartylglutamate
activates cyclic-AMP coupled metabotropic glutamate receptors in
cerebellar astrocytes. Glia 24: 172-180, 1998.
Ghose S., Wroblewska B., Corsi L., Grayson D. R., De Blas A. L., Vicini
S., Neale J. H. NAAG stimulates mGluR3 to regulate expression of the
GABAA ?6 subunit in cerebellar granule cells. J. Neurochemistry 69: 2326-
2336, 1997.
Bzdega T., Turi T., Wroblewska B., She D., Neale J. H. Molecular cloning
of a peptidase against N-Acetylaspartylglutamate (NAAG) from a rat
hippocampal cDNA library. J. Neurochemistry 69: 2270-2278, 1997.
Wroblewska B., Wroblewski J. T., Pshenichkin S., Surin A., Sullivan S.
E., Neale J. H. N-Acetylaspartylglutamate selectively activates mGluR3
receptors in transfected cells. J. Neurochemistry 69: 174-182, 1997.
Dong Q. S., Wroblewska B., Myers A. K. Inhibitory effect of alcohol on
cyclic GMP accumulation in human platelets. Thrombosis Research, 80: 143-
151, 1995.
Wroblewska B., Wroblewski J. T., Saab O. H., Neale J. H. N-
acetylaspartylglutamate inhibits forskolin-stimulated cyclic AMP levels
via a metabotropic glutamate receptor in cultured cerebellar granule
cells. J. Neurochemistry, 61: 943-948, 1993.
Vargas R., Wroblewska B., Rego A., Hatch J., Ramwell P. W. Oestradiol
inhibits smooth muscle cell proliferation of pig coronary artery. Br. J.
Pharmacology, 109: 612-617, 1993.
Rego A., Vargas R., Wroblewska B., Foegh M. L., Ramwell P. W.:
Attenuation of vascular relaxation and cGMP by cyclosporin A. J.
Pharmacol. Exp. Ther. 252: 165-170, 1990.
Vargas R., Bormes G. W., Wroblewska B., Foegh M. L., Kot P.A., Ramwell P.
W.: Angiopeptin inhibits thymidine incorporation in rat carotid artery in
vivo and in vitro. Transplantation Proc. 21:3702-3704, 1989.
Thomas G., Vargas R., Wroblewska B., Ramwell P. W.: Role of the
endothelium and arginine peptides on the vaso-motor response of porcine
internal mammary artery. Life Sciences 44:1823-1830, 1989.
Wroblewska B., Kempski O., Merkel N., Bembry J., Spatz M.: Effect of
vasoactive peptides on prostacyclin formation in cerebrovascular cellular
elements and glia. A comparative study. Neurochem. Int. 12: 1-4, 1988.
Kempski O., Wroblewska B., Spatz M.: Effects of forskolin on growth and
morphology of cultured glial and cerebro-vascular endothelium and smooth
muscle cells. Int. J. Devl. Neuroscience.5: 435-445, 1987.
Uotila P., Vargas R., Wroblewska B., d'Alarcao M., Matsuda S. P., Corey
E. J., Cunard C. M., Ramwell P. W.: Relaxing effects of 15-lipoxygenase
products of arachidonic acid on rat aorta. J. Pharmacol. Exp. Ther. 242:
945-949, 1987.
d'Alarcao M., Corey E. J., Cunard C., Ramwell P. W., Uotila P., Vargas
R., Wroblewska B.: The vasodilatation induced by hydroperoxy metabolites
of arachidonic acid in the rat mesenteric and pulmonary circulation. Br.
J. Pharmacol. 91: 627-632, 1987.
Wroblewska B., Kumami K., Cvejic V., Mrsulja B. B., Spatz M.:
Cerebrocortical modulation of S2-receptors and turnover rate of 5-
hydroxytryptamine in ischemia. Neurochem. Res. 12: 21-25, 1987.
Spatz M., Mrsulja B. B., Wroblewska B., Merkel N., Bembry J.: Modulation
of glycogen metabolism in cerebromicro-vascular smooth muscle and
endothelial cultures. Biochem. Biophys. Res. Commun. 134: 484-491, 1986.
Wroblewska B., Spatz M., Merkel N., Bembry J.: Cerebro-vascular smooth
muscle II: Characterization of adrenergic receptors linked to adenylate
cyclase. Life Sci. 34; 783-781, 1984.
Albrecht J., Wroblewska B., Mossakowski M. J.: The binding of insulin to
cerebral capillaries and astrocytes of the rat. Neurochem. Res. 7: 489-
494, 1983.
Skubiszewski B., Przekop F., Wolinska-Witort E., Stupnicka E., Wroblewska
B., Domanski E.: Effect of prolonged administration of small doses of LH-
RH and LH-RH analogue d-Ser(But)6-Des-Gli-NH2-10-ethylamide on the
release of LH and induction of ovulation in the mid-anoestrous ewes. J.
Animal Reprod. 4: 269-274, 1982.
Wroblewska B., Domanski E.: Role of the medial-basal hypothalamus in the
secretion of growth hormone during pregnancy and lactation in ewes. J.
Endocrinol. 89: 349-354, 1981.
Wroblewska B., Wolinska-Witort E., Domanski E.: The effect of dopamine on
the release of the prolactin in sheep with lesions of the hypothetical
centre producing prolactin inhibiting factor (PIF). Acta Physiol. Pol.
31: 333-339, 1980.
Domanski E., Przekop F., Skubiszewski B., Wroblewska B., Stupnicka E.:
Effect of prolonged infusion of small doses of LH-RH on the release of LH
and ovulation in ewes during midanoestrous. J. Reprod. Fert. 51: 457-460,
1977.
Book chapters and publications in other journals:
Wroblewski J. T., Lazarewicz J. W., Wroblewska B., Costa E.: Role of
arachidonic acid in signal transduction of N-methyl-D-aspartate-sensitive
glutamate receptors. In: Meldrum B. S., Moroni F., Simon R. P., Woods J.
H. (eds.) Excitatory Amino Acids, pp. 231-240, Raven Press, New York,
1991.
Wroblewski J. T., Raulli R., Lazarewicz J. W., Kiedrowski L., Costa E.,
Wroblewska B.: Intracellular messengers generated by glutamate receptors
in primary cultures of cerebellar neurons. In: Transmitter Amino Acid
Receptors: Structures, Transduction and Models for Drug Development (ed.
E.A. Barnard and E. Costa), Thieme Med. Publ., pp. 379-393, 1991.
Vargas R., Thomas G., Wroblewska B., Ramwell P. W.: Differential effects
of 17? and 17? estradiol on PGF2? mediated contraction of the porcine
coronary artery. Advances in Prostaglandin, Tromboxane, and Leukotriene
Research, (ed. Samuelsson, Paoletti, Ramwell) Vol 18, pp.245-248, 1989.
Spatz M., Yamamoto H., Lust D. W., Wroblewska B., Merkel N., Bembry J.
Cerebral microvessels. In: Peptide and amino acid transport mechanisms in
the central nervous system (ed. Rakic L., Begley D.J., Davson H. and
Zlokovic B.V.), The Macmillan Press, 1988.
Spatz M., Yamamoto H., Mrsulja B. B., Lust D. W., Wroblewska B., Merkel
N., Bembry J.: Effect of vasoactive substances on carbohydrate metabolism
in cerebro-vascular cultures. In: Stroke and Microcirculation (ed. Cervos-
Navarro), pp. 205-209, 1987.
Wroblewska B., Spatz M.: Interaction between adenosine and prostaglandin
receptors in capillary endothelial cultures. In: Stroke and
Microcirculation (ed. Cervos-Navarro), Raven Press, pp. 235-238, 1987.
Spatz M., Wroblewska B., Mrsulja B. B., Merkel N., Bembry J.: Biochemical
characteristics of explanted and propagated cerebromicrovascular
endothelium and smooth muscle cells. In: Neural Regulation of Brain
Circulation (ed. Owman and Hardebo), Elsevier Sci. Publ., pp.43-57, 1986.
Spatz M., Cvejic V., Kumami K., Ueki Y., Djuricic B. M., Wroblewska B.,
Mrsulja B. B.: Neurotransmitters in ischemia with particular reference to
5-hydroxy-tryptamine. In: Pharmacology of Cerebral Ischemia (ed.
Krieglstein), Elsevier Publ. Sci., pp.181-190, 1986.
Wroblewska B., Ueki Y., Mrsulja B. B., Djuricic B., Spatz M.: Serotonin
S2-receptors in ischemic brain edema. In: Brain Edema (ed. Inaba, Klatzo,
Spatz) pp. 178-184, 1985.
Spatz M., Maruki Ch., Nagatsu I., Wroblewska B., Mocarski E., Merkel N.,
Bembry J.: Recent progress in the studies related to cerebral
microvascular function. J. Cereb. Blood Flow Metab. 3: S311-S312, 1983.
Abstracts presented at the meetings
Wroblewska B., Wegorzewska I., Bzdega T., Neale J.H. Differential coupling
to cyclic nucleotide transduction systems between mGluR3 and mGluR2
receptors expressed in the C6 glioma cell line. 38th Annual Meeting,
Society for Neuroscience, Washington DC, 2008.
Wroblewska B., Olszewski R.T., Bukhari N., Bzdega T., Neale J.H.,
Kozikowski A.P., Zhou J. NAAG and mGluR3 receptor in neuropatic pain and
schizophrenia animal models., Polish Neuroscience Society, Krakow, 2005.
Olszewski R.T., Wegorzewska M.M., Monteiro A.C.C., Kozikowski A.P., Sarva
J., Wroblewska B., Neale J.H. NAAG peptidase inhibitor reduces
positive and negative schizophrenia - like symptoms induced by PCP in
mouse models. 35th Annual Meeting, Society for Neuroscience, Washington
DC, 2005.
Gehl L.M., DeStefano M.W., Bzdega T., Wroblewska B., Olszewski R.T.,
Burbello P., Neale J.H. Expression patterns of two N-
acetylaspartylglutamate - hydrolyzing enzymes: Glutamate Carboxypeptidases
II and III. 35th Annual Meeting, Society for Neuroscience, Washington DC,
2005.
Olszewski R.T., Ramadan E., Bzdega T., Bacich D.J., O'Keefe D.S., Heston
W.D.W Wroblewska B., Neale J.H. Characterization of N-
acetylaspartylglutamate peptidase II as a potential factor in survival of
glutamate carboxypeptidase II (GCPII) knockout mice. 33rd Annual Meeting,
Society for Neuroscience, New Orleans, LA, 2003.
Wroblewska B., Ramadan E., Bacich D.J., O'Keefe D.S., Heston W.D.W,
Bukhari N., Wegorzewska I., Bzdega T., Wroblewski J.T., Kozikowski A.,
Yamamoto T., Neale J.H. Probing the functions of NAAG via NAAG peptidases
and mGluR3. 33rd Annual Meeting, American Society for Neurochemistry, Palm
Beach, FL, 2002.
Wroblewska B., Shamimi-Noori S., Bzdega T., Wroblewski J.T., Neale J.H. N-
acetylaspartylglutamate (NAAG) inhibits cGMP formation through the
activation of mGluR3 receptor. 32nd Annual Meeting, Society for
Neuroscience, Orland, FL, 2002.
Bzdega T., Crowe S., Wroblewska B., Rafalski V., Neale J.H. Molecular
cloning and expression of novel brain peptidase with activity against N-
acetylaspartylglutamate. 32nd Annual Meeting, Society for Neuroscience,
Orlando, FL, 2002.
Bzdega T., O'Keefe D., Bacich J.D., Ramadan E., Wroblewska B., Bukhari N.,
Wegorzewska I., Heston W.D.W., Neale J.H. Generation and characterization
of mice lacking the glutamate carboxypeptidase II. 31st Annual Meeting,
Society for Neuroscience, San Diego, CA, 2001
Zhao J., Ramadan E., Cappiello M., Neale J.H., Bzdega T., Wroblewska B.
NAAG inhibits KCl-induced [3H]-GABA release in cultured rat cortical
neurons. 30th Annual Meeting, Society for Neuroscience, New Orleans LA,
2000.
Wang H., Bzdega T., Neale J.H., Wroblewska B. Changes in carboxypeptidase
II activity and expression in cortical and cerebellar astrocytes in
culture. 30th Annual Metting, Society for Neuroscience, New Orleans LA,
2000.
Wroblewska B., Bzdega T., Synthetic analogs of NAAG are potent blockers
29th Annual Meeting Society for Neuroscience, Miami Beach, Fl, 1999.
Wroblewska B., Neale J.H., Bzdega T., Lea P.M., Sarvey J.M. Antagonistic
effects of beta-NAAG on mGluR3 receptor. 3rd International Meeting on
Metabotropic Glutamate Receptors, 19-24 September, Taormina, Italy, 1999.
Wroblewska B. NAAG as an agonist of metabotropic glutamate receptor -
mGluR3, 4th International Congress of the Polish Neuroscience Society, 2-5
September, Gdansk, Poland, 1999.
Lea P.M. IV, Wroblewska B., Neale J.H., Sarvey J.M. ?-NAAG rescues LTP
from blockade by NAAG in rat dentate gyrus via the mGluR3 receptor. 28th
Annual Meeting Society for Neuroscience, Los Angeles, CA, 1998.
Wroblewska B., Neale J.H., Bzdega T., Lea P.M. IV, Sarvey J.M.
Antagonistic effects of ?-NAAG on mGluR3 receptor. 28th Annual Meeting
Society for Neuroscience, Los Angeles, CA, 1998.
Bzdega T., She D., Turi T.,Wroblewska B., Neale J.H. Molecular cloning of
alternatively spliced variants of the peptidase against N-
acetylaspartylglutamate (NAAG) from human and rat nervous systems. 28th
Annual Meeting Society for Neuroscience, Los Angeles, CA, 1998.
Wroblewska B., Sullivan S. E., Bzdega T., Neale J. H. Role of N-
acetylaspartylglutamate (NAAG) in the neurotransmittion. 3rd International
Congress of the Polish Neuroscience Society, 7-10 September, Lodz, Poland,
1997.
Sullivan S. E., Surin A., Wroblewska B., Wroblewski J. T., Neale J. H.
Inhibitory effect of N-Acetylaspartylglutamate (NAAG) on intracellular
Ca2+ concentrations in cultured neuronal cells. 27th Annual Meeting
Society for Neuroscience, New Orleans, 1997.
Ghose S., Wroblewska B., Corsi L., De Blas A., Vicini S., Neale J. H. NAAG
differentially modulates expression of GABAA ?3, ?2L, and ? subunit mRNA
in cultured cerebellar granule cells. 27th Annual Meeting Society for
Neuroscience, New Orleans, LA, 1997.
Wroblewska B., Bruno V., Wroblewski J. T., Nicoletti F. Neuroprotective
activity of NAAG in mixed cultures of cortical cells. 27th Annual Meeting
Society for Neuroscience, New Orleans, LA, 1997.
Wroblewska B., Bzdega T., Sullivan S. E., Bruno V., Nicoletti F., She D.,
Wang F., Wroblewski J. T., Ghose S., Neale J. H. NAAG selectively
activates mGluR3 to inhibit voltage-dependent calcium channels in neurons,
to reduce NMDA-induced neurotoxicity, and to stimulate astrocyte mitosis.
ISN/ASN satellite symposium and ASPET Colloquium on Excitatory Amino
Acids, Waterville Valley, July 16-20, 1997.
Ghose S., Wroblewska B., Grayson D. R., Neale J. H. NAAG modulates
expression of the GABA-A ?6 subunit mRNA via activation of a mGluR in
cultured cerebellar granule cells. 26th Annual Meeting Society for
Neuroscience, Washington DC, 1996.
Wroblewski J. T., Pshenichkin S., Wroblewska B. Pharmacological
characterization of mGluR6 metabotropic glutamate receptors expressed in
CHO cells. 26th Annual Meeting Society for Neuroscience, Washington DC,
1996.
Wroblewska B., Surin A., Pshenichkin S., Wroblewski J. T., Neale J. H.
Selectivity of the effects of N-acetylaspartylglutamate (NAAG) on mGluR3
metabotropic glutamate receptors. 26th Annual Meeting Society for
Neuroscience, Washington DC, 1996.
Wroblewska B., Santi M. R., Neale J. H. NAAG activates cloned mGluR3
receptor. 25th Annual Meeting Society for Neuroscience, San Diego, CA,
1995.
Santi M. R., Wroblewska B., Neale J. H. NAAG acts through metabotropic
glutamate receptors in cultured glial cells. 25th Annual Meeting Society
for Neuroscience, San Diego, CA, 1995.
Mehta V., Razavi P., Tamjidi P., Datwani A., Scharaf R., Wroblewska B.,
Namboodiri M. A. A. N-acetylaspartate as an acetyl source for lipid
synthesis in cultured astrocytes from 8 day old rats. 25th Annual Meeting
Society for Neuroscience, San Diego, CA, 1995.
Wroblewski J. T., Ikonomovic S., Santi M. R., Wroblewska B., Grayson D.
R.: Expression of metabotropic glutamate receptors coupled to adenylate
cyclase in cerebellar neurons. 23rd Annual Meeting Society for
Neuroscience, Washington D.C., 1993.
Neale J. H., Wroblewska B.: N-acetylaspartylglutamate inhibits cyclic GMP
formation in cerebellar granule cells. 23rd Annual Meeting Society for
Neuroscience, Washington D.C., 1993.
Wroblewska B., Neale J. H., Wroblewski J. T.: Excitatory amino acids
inhibit membrane-bound guanulate cyclase in cerebellar neurons. 23rd
Annual Meeting Society for Neuroscience, Washington D.C., 1993.
Wroblewski J. T., Majewska M., Wroblewska B.: Metabotropic glutamate
receptors coupled to adenylyl cyclase decrease forskolin-stimulated cAMP
formation in cerebellar granule cells. 22nd Annual Meeting Society for
Neuroscience, Anaheim, CA, 1992.
Wroblewska B., Neale J. H.: NAAG decreases forskolin-stimulated cAMP
formation in cerebellar granule cells. 22nd Annual Meeting of the Society
for Neuroscience, Anaheim, CA, 1992.
Vargas R., Wroblewska B., Ramwell P. Angiopeptin inhibits proliferation in
segments of porcine coronary artery. American College of Cardiology, 40th
Annual Scientific Session, Atlanta, GA, 1991.
Wroblewski J. T., Lazarewicz J. W., Costa E., Wroblewska B.: Role of
arachidonic acid in signal transduction of N-methyl-D-aspartate receptors.
Neurochem. Int.16 (Suppl.1):61, 1990.
Wroblewska B., Majewska M., Costa E., Wroblewski J. T. Dual regulation of
guanylate cyclase by N-Methyl-D-Aspartate receptors: role of nitric oxide
and arachidonic acid. 20th Annual Meeting Society for Neuroscience, St.
Louis, MO, 1990
Wroblewska B., Ramwell P. W., Costa E., Wroblewski J. T. 12-Lipoxygenase
metabolites mediate stimulation of guanylate cyclase by glutamate
receptor. Prostaglandins and Related Compaunds, Florence, Italy, May 1990.
Vargas R., Bormes G., Wroblewska B., Kot P., Ramwell P. W., Foegh M., L.:
Angiopeptin, a somatostatin analog inhibits in vivo and in vitro
myointimal proliferation in rat carotid artery. 62nd American Heart
Association, New Orleans LA, 1989.
Wroblewska B., Wroblewski J. T., Ramwell P. W., Costa E.: Lipoxygenase
mediates the formation of cyclic GMP induced by excitatory amino acids in
cerebellar granule cells. 19th Annual Meeting Society for Neuroscience
Phoenix, AR, 1989.
Rego A., Vargas R., Wroblewska B., Foegh M. L., Ramwell P. W.: Effect of
cyclosporin A (CSA) on vascular relaxation. Third International Alexis
Carrel Conference: Accelerated Atherosclerosis, March 30-31 1989.
Rego A., Vargas R., Wroblewska B., Foegh M. L., Ramwell P. W.: Effects of
cyclosporin A on resistance vessels reactivity. Circulation 78:II552,
1988.
Vargas R., Thomas G., Wroblewska B., Ramwell P. W.: Differential effects
of 17? and 17? estradiol on PGF2? mediated contraction of the porcine
coronary artery. Taipei Conference on Prostaglandin and Leukotriene
Research, Taipei, Taiwan, April 22-24, 1988.
Rego A., Vargas R., Foegh M. L., Wroblewska B., Ramwell P. W.: Cyclosporin
A treatment alters vascular reactivity in the rat. Second International
Congress on Cyclosporine. Washington DC, Nov 4-7, 1987.
Spatz M., Yamamoto H., Lust D. W., Wroblewska B., Merkel N., Bembry J.:
Peptides and cerebral microvessels. Int Symposium: Peptide and amino acid
transport mechanisms in the central nervous system, Belgrade, Yugoslavia,
May 3-7, 1987.
Wroblewska B., Kempski O., Spatz M.: Cultured cerebro-microvascular smooth
muscle and glial cells: prostaglandin receptors linked to adenylate
cyclase. 16th Annual Meeting Society for Neuroscience, Washington D.C.,
1986.
Vargas R., Wroblewska B., Cunard C.M., Uotila P., Ramwell P. W.:
Lipoxygenase derivatives of free fatty acid induce relaxation of rat
aorta. FASEB, Washington D.C., 1986.
Wroblewska B., Cunard C. M., Vargas R., Ramwell P. W.: Forskolin and dB-
cAMP-induced relaxation of rat aorta. FASEB, Washington D.C., 1986.
Wroblewska B., Kempski O., Merkel N., Bembry J., Spatz M.: Prostaglandin
synthesis (PGI2) in cultured microvascular cellular elements and glia from
rat brain: effects of angiotensins and bradykinin. 15th Annual Meeting
Society for Neuroscience, Dallas TX, 1985.
Kempski O., Wroblewska B., Kretzschmar H., Merkel N., Bembry J., Spatz M.:
Forskolin induces growth inhibition and differentiation in cultured cells
from rat brain. 15th Annual Meeting Society for Neuroscience, Dallas TX,
1985.
Spatz M., Wroblewska B., Mrsulja B. B., Merkel N., Bembry J.: Biochemical
characteristics of explanted and propagated cerebrovascular endothelium
and smooth muscle cells. Symposium in Neural Regulation of Brain
Circulation, Lund, Sweden, 1985.
Wroblewska B., Spatz M., Bembry J.: Binding of alpha-adrenergic receptors
in cultured cerebrovascular smooth muscle cells. 14th Annual Meeting
Society for Neuroscience, Anaheim CA, 1984.
Spatz M., Wroblewska B., Bembry J.: Recent progress in the cerebrovascular
receptors. 2nd European Conference for Neuropathology, Warsaw, Poland,
1984.
Szumanska G., Spatz M., Wroblewska B., Bembry J.: Histochemical detection
of adenylate cyclase activity with forskolin. 13th Annual Meeting of the
Society for Neuroscience, Boston MA, 1983.
Wroblewska B., Domanski E.: Effect of central nervous system on the
secretion of growth hormone in the ewes during pregnancy and lactation.
Meeting on Peptides and Brain Function, Lodz, Poland, 1979.
Domanski E., Polkowska J., Wolinska-Witort E., Wroblewska B.: Hypothalamic
control of prolactin secretion. 6th International Congress of
Endocrinology, Melbourne, Australia, 1980.
Domanski E., Wolinska E., Wroblewska B.: The role of dopamine in the
regulation of prolactin secretion in sheep. 7th Meeting on Physiology of
Farm Animals, Smolenice, Czechoslovakia, 1975.
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