Assistant Quality Control

NATALIA GORDONOV

** ********* ******, **** *******, NJ 08520)

tel.: 609-***-****; e-mail: *******@*****.***

SUMMARY

Well organized, detail oriented Biologist with extensive experimental

biology experience across pharmaceutical and academic environments in

therapeutic areas of inflammation, neuroscience and oncology. Practical

expertise in a variety of methods in the fields of molecular biology,

biochemistry and immunology. Dynamic communicator, and an excellent

leader and team player.

TECHNICAL SKILLS

- METHODS OF MOLECULAR BIOLOGY: Isolation of DNA and RNA and analysis of

their structure (electrophoresis, Northern, Southern and Western

blotting, hybridization with radiolabeled probes, PCR and RT-PCR, PAGE),

transformation, transfection, plasmid cultivation and purification.

- IN VITRO: Methods of cell culture for growing different cells and cell

lines; harvesting and processing animal tissue; cell-based assays,

primary tissue culture.

- IMMUNOLOGICAL METHODS: ELISA, IP; separation of lymphocytes from blood

using Ficoll gradient.

- METHODS OF BIOCHEMISTRY: Protein purification (including column

chromatography), SDS PAGE, BCA.

PROFESSIONAL EXPERIENCE

VAXINNATE Inc., Cranbury, NJ

2010

Research Associate, Immunological group

. Performed ELISA on clinical trial samples; RNA purification, PCR.

. Provided data analysis and troubleshooting.

. Followed biosafety procedures for handling human blood and its

components.

. Applied GLP and GMP during execution of all working tasks.

UMDNJ, Newark, NJ

2010

Research teaching specialist IV, Department of pharmacology and physiology

Studied the role of amyloid precursor protein in the pathogenesis of

Alzheimer's disease.

. Performed Western blotting, plasmid purification and

immunocytochemistry.

. Provided troubleshooting.

LIFECELL, Branchburg, NJ (contract position)

2008 Assistant

Scientist, R&D Department

. Grew and conducted experiments with cell lines.

. Tissue processing, DSC, enzyme digestion assays (collagenase

susceptibility, Maillard reaction, etc.)

. Performed data analysis and troubleshooting.

CENTOCOR (JNJ company), Raritan, NJ

2006 - 2008

Associate Scientist, Department of Analytical Services

Key team member of Erythropoietin method quality control group

. Performed Mouse Bioassay for Erythropoietin, dosing of mice (EPO and

Fe59), using different routes of administration; cardiac puncture and

hematocrits procedures; estimated sample concentration using

spectrophotometer.

. Documented results in LIMS, released test results.

. Tracked data trending, performed investigations, and reviewed SOPs.

INSTITUTE FOR BASIC RESEARCH IN

DEVELOPMENTAL DISABILITIES, Staten Island, NY 2003 - 2006

Research Assistant Scientist I, Department of Developmental Biochemistry

Studied the role of Taurine Cloramine in the development of inflammation.

. Optimized tissue culture methods for growing and performing experiments

with different cells and cell lines, used cell based assays (cell

proliferation).

. Performed dissection techniques; transformation and plasmids

purification.

. Isolated proteins/DNA/RNA and analyzed their structure (electrophoresis,

PAGE, Northern and Western blotting, hybridization with radiolabeled

probes, EMSA).

. Trained team members in various methods.

PRINCETON UNIVERSITY, Princeton, NJ 2001 - 2002

Laboratory Assistant, Department of Molecular Biology

Member of oncology research team.

. Determined the role of NDP Kinase in the development of cancer.

. Used methods of biochemistry and molecular biology for protein and

nucleic acids research: protein purification (including column

chromatography), SDS PAGE, and protein assays (Bradford, BCA),

transformation and PCR

ALCORN STATE UNIVERSITY, Alcorn, MS 1999 - 2001

Laboratory Associate, Department of Biological Sciences

Supervised up to 4 graduate students in Immunology laboratory research

under the direction of professor.

. Determined immunomodulation effect of Met-enkephalin and related peptides

on cytokine production in vitro and in vivo.

. Optimized ELISA and cell culture techniques for determination of

different cytokines.

. Developed a new animal model for studying DTH response on guinea pigs:

dosing, dissection and histology techniques.

EDUCATION AND PROFESSIONAL DEVELOPMENT

. MS, (Biology) - The First Moscow Medical Academy, Moscow, Russia

. Course on GMP / GLP - Centocor, NJ, USA

ADDENDUM

PUBLICATIONS AND PRESENTATIONS

1. M.R. Quinn, V. Serban, Y. Liu, N. Gordonov, and C. Wang. Taurine

Chloramine Inhibites NO Production and INOS Gene Expression in BV-2 Cells

by Attenuating NF-kB Activation.-Keystone Symposia: NF-kB: 20 years on the

Road From Biochemistry to pathology, Baff, Alberta, Canada, March 2006.

2. Sizemore RC, Piva M, Moore L, Gordonov N, etc., Modulation of Delayed-

Type Hypersensitivity Responses in Hairless Guinea Pigs by Peptides Derived

from Enkephalin, Neuroimmunomodulation, 2004, N11, 141-148.

3. Cobb A, Moore L, Godfrey H, Gordonov N, etc. The Use of Hairless (IAF/HA-

Ho) Guinea Pigs for the Determination of Delayed-type Hypersensitivity to

PPD. - International Immunopharmacology, v.1, N2, 2001, 349-353.

4. Montgomery C, Gordonov N, Sizemore RC. The Effects of Met-enkephalin and

Related Peptides on Interleukin-2 (IL-2) production.- Mississippi Academy

of Sciences, University of Southern Mississippi, April 2000.

Contact this candidate