Project Management

? abhcnq@r.postjobfree.com ? 443-***-**** ?

Yuri Kim

* ****** *** . ***********, ** 21093 . abhcnq@r.postjobfree.com .

home: 410-***-**** . cell: 443-***-****

Highly skilled research scientist with expertise in studies involving

optical microscopy, 3D-imaging, single-particle tracking and image

analysis, cell-based assays, application of various biophysical methods,

spectroscopy and chromatography for protein and chemical analysis, computer-

based massive data mining and analysis with technical and project

management skills seeking new carrier opportunities

Professional Experience

RESEARCH ASSOCIATE July 2010-February 2011

DEPARTMENT OF NEUROSCIENCES, SCHOOL OF MEDICINE GEORGETOWN UNIVERSITY,

WASHINGTON, DC

-Developing real-time fluorescent imaging of dendritic spine shape dynamics

and calcium signaling in mouse brain neurons.

-Provide technical support for the users of confocal microscopy facility in

the department.

-Perform data collection and analysis of immunofluorescent and

immunohistochemical staining and 3D-imaging and reconstruction of protein

distribution in isolated neurons and astrocytes.

RESEARCH ASSOCIATE October 2006-June 2010

Institute of Human Virology, School of Medicine University of Maryland

Baltimore, MD

-Lead the innovative project of live imaging and real-time 3-D tracking of

the interaction and fusion of single human viruses, HIV-1 particularly,

with human cells. These efforts resulted in RO1 NIH grant award to PI and

manuscript publication in Cell.

-Perform cell transfection, immunofluorescent staining and 3-D imaging of

cellular and viral proteins distribution in the cells.

-Provide expert support of researchers utilizing microscopy facility,

organization of demo of the new microscopy systems.

-Responsible for data handling, storage and analysis of massive imaging

database by using cloudy Data Robotics (DROBO) system, single-particle

tracking and analysis software.

RESEARCH ASSOCIATE, July 2004-October 2006

Department of Pediatrics Infectious Diseases School of Medicine, Johns

Hopkins University Baltimore, MD

-Designed and performed experiments with pathogenic microorganisms and

human cells in vitro using real-time fluorescent imaging, confocal

microscopy and fluorescent immunostaining.

-Lead the project on role of cell calcium signaling system in the

transmigration of causative parasite of the African sleeping sickness

across in vitro blood-brain barrier model.

-Perform custom upgrading of imaging microscopy workstation with perfusion

system and temperature-controlled incubator.

-Provide technical and expert support in data collection and analysis of

multiple users of imaging microscopy workstation within the large

laboratory.

POST-DOCTORAL FELLOW July 2000-June 2004

Department of Pediatrics Infectious Diseases

School of Medicine, Johns Hopkins University Baltimore, MD

-Lead the project on real-time interaction of microbial pathogens with

primary human brain microvascular endothelial cells (HBMEC)

-Studied mechanisms of thrombin effects on HBMEC resulting in blood-brain

barrier dysfunction (awarded grant from American Heart Association)

-Developed a new in vitro model of human blood-brain barrier by using

primary HBMEC and astrocytes under static and fluid shear stress.

RESEARCH SCIENTIST August 1997-July 2000

Institute of Cell Biophysics Russian Academy of Sciences Pushchino, Moscow,

Region, Russia

-Design and custom build single-cell, real-time fluorescent and

electrophysiological measurement system for studies of the mechanism of

taste reception;

-Lead investigation of the role of ATP receptor, newly found in mouse taste

bud cells, in taste reception (this study received in 2001 Howard Hughes

Medical Institution five-year grant for PI).

Additional EXPERIENCE

GUEST RESEARCHER, Department of Biomedical and Behavioral Sciences,

National Institute for Occupational Safety and Health, Cincinnati, OH, USA,

1996-1997.

PROFESSIONAL SKILLS

In-depth knowledge of light, fluorescent, confocal and deconvolution

microscopy; various image analysis software; programming macros for

analysis;

Hands-on experience with protein biochemistry, HPLC, spectroscopy,

analytical instrumentation, working with almost 2 dozen cell types and

microbial pathogens;

Technical expertise and troubleshooting of various computer/instrumentation

hardware and software;

Strong background in biophysics and chemistry allows fast learning and

understanding of new techniques and methods;

Flexible, highly analytical and problem-solving skills;

Excellent presentation, writing, and communication skills;

Project management skill; supervisor of graduate students and post-docs.

EDUCATION AND TRAINING

-Ph.D. in Biophysics --- Institute of Biophysics Russian Academy

of Sciences Pushchino, Moscow Region, Russia

-Master of Science in Biochemistry --- Dnepropetrovsk State University

Ukraine Graduated with honors.

AWARDS, FELLOWSHIPS AND GRANTS

-Scientist Development Grant from American Heart Association National

Research Programs, 2004-2009.

-Annual Research Associateship Awards from the National Research Council

(Washington, DC, USA) and National Institute for Occupational Safety and

Health CDC, DHHS (Cincinnati, OH, USA) in 1993, 1994 and 1995 to study in

vitro effects of low-intensity, low frequency electromagnetic fields on

biological objects;

Professional activities

Author of 27 publications, including publications in such high rank

journals, as Cell, J. Clinical Investigation, J. Cell Science, Cellular

Microbiology, etc.

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