Manager Assistant

Sudharani Nagaraju

Dublin, CA *****

847-***-**** (cell) *******@*****.***

Education

M.S. in Biological Sciences, California State University, Hayward, CA

(March 2010)

Biotechnology Certification Program (BCP), California State

University, Hayward, CA

(June 2008)

M.A. in Mass Communication, Bharathiar University, India

B.S. in Zoological Sciences, Bharathiar University, India

Work / Research Experience

Research Assistant, CSU East Bay, MBRS-Score Program (Jan 2009 thru

May 2009)

. Performed Molecular techniques such as Isolation of genomic

DNA, Plasmids, and RNA, PCR, Real-time PCR, Cycle Sequencing,

TOPO cloning, Gateway recombination and transformations,

quantified nucleic acids using nanodrop using pico green and

spectrophotometer.

. Used various data mining and analyzing tools such as Blast,

APE, Chromas, Lalign and clustalW.

. Assisted a Professor in characterizing nutrient-stress

responsive genes.

Research Work (Jun 2008 - Mar 2010)

Worked as a Lab Manager

. Guided students with lab & research work and maintained the

lab.

Thesis: Characterization of the promoter of a Nutrient-stress

Responsive Gene in white lupin

. Performed primer-walk sequencing to obtain the putative

promoter of L#315 a nutrient stress responsive gene.

. Performed TOPO cloning and Gateway recombination techniques

to do transcriptional fusion of L#315 promoter with GUS and

GFP reporter genes independently and transformed the

constructs into white lupin plants.

. Performed Real-time PCR to qualitatively analyze the

presence of GUS and GFP reporter gene in the proteoid roots

of the transformed plants. Also compared the expression

level of the gene of interest in nutrient stress and

nutrient sufficient condition.

. Used Agarose gel electrophoresis to confirm the PCR

products.

. Used histochemical assay to visualize GUS expression &

Fluorescence Microscope to observe GFP expression in the

proteoid roots of the transformed plants.

Skills Summary

PCR, Sequencing and Analysis

. Purification and Isolation of genomic DNA, Plasmids, and RNA

. PCR

. Reverse Transcriptase PCR

. Real Time PCR

. Cloning

. Gateway Recombination techniques

. Agarose

. SDS-PAGE Gel Electrophoresis

. Quantification using spectrophotometer and nanodrop

. Site-directed Mutagenesis - creating mutant entry clone and

recombining with destination vector using gateway technology

. Transformation techniques

. Cycle Sequencing

. RNAi interference

. Histochemical assay

Cell culture Techniques

. Cell line maintenance and sub-culturing

. Establish primary culture from mouse heart and lung cells

. Clonal selection using limiting dilutions and clonal rings

Protein Chemistry

. Protein isolation and purification using column, ion

exchange and affinity chromatography

. Bradford assay to calculate the protein concentration

. Separation of biomolecules using HPLC technique

. SDS Page

. Western blotting

Proficient with Microsoft office and data-mining tools like Chromas,

Clustal alignment, Blast, Expasy translate tools, ORF finder etc. to

analyze sequence obtained.

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