Cheryl Guadagna
____________________________________________________________________________
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* ***** ********** ******* ** a biotechnology and academic research
laboratory setting.
--Professional Experience--
UC Davis: Cell Biology & Human Anatomy, Deng Lab Nov 09 to Nov 10
Junior Specialist - Assistant to postdoctoral researcher
(Manuscript in progress, see below)
. Managed mouse colony; responsibilities included genotyping by toe/tail
clip, breeding, weaning, drug administration & daily care of animals.
Assisted with CNS surgical procedures and after-care.
. Skill in brain and spinal cord tissue extraction for glial cell primary
culture or cryo-embeding & sectioning by microtome. Experience with
confocal microscopy and immunohistochemistry.
. DNA and RNA extraction for use in PCR, qRT-PCR and cloning.
. Maintained and organized laboratory supplies and record keeping of waste
disposal for lab.
UC Davis: CNPRC, Abel Lab May 08
- June 09
Staff Research Associate I
. Isolation and primary culture of lymphocytes from primate tissues,
primate biopsy & necropsy.
. Characterization of immune cells by flow cytometry (FACS). Experience
with Flo-Jo computer program.
. Maintenance of equipment and laboratory stock and assistance of GSRs with
their projects.
Antibody Solutions, Inc. Sept
07 - Feb 08
Research Associate (Temporary Assignment)
. Characterization of hybridoma clones in monoclonal antibody production.
. Antibody purification and characterization by ELISA and western blot.
Santa Cruz Biotechnology, Inc.
Nov 05 - Sept 07
Research Associate
. Purification of polyclonal secondary antibodies from animal sera by
affinity chromatography.
. Conjugation of secondary antibodies with HRP and flurochromes,
. Preparation of antigen inoculations for production animals and product
kit assembly.
Research Assistant
. Preparation of animal sera, buffers, media & other solutions.
. Product preparation & kit assembly, record keeping and maintenance of
department stocks.
Laboratory Support
. Organization, sterilization and maintenance of equipment, glassware and
general supplies in support of 5 labs.
Veterinary Centers of America, Hemingway Cat Hospital Sept 02 -
May 04
Technician's Assistant
. Care, feeding, medication and observation of facility-boarded animals.
. Assisted with procedures including gentle restraint of animals, blood
drawing and subcutaneous injections.
--Manuscripts--
V Selvaraj, M Horiuchi, C Guadagna, N Phuong, T Itoh, K Otsu and W Deng
p38? Signaling in Oligodendrocyte Development and Myelination [Manuscript
in preparation]
--Education--
B.S. in Biology University of California, Santa Cruz, CA