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Assistant Maintenance

Location:
Los Gatos, CA, 95030
Posted:
December 10, 2010

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Resume:

Cheryl Guadagna

408-***-****

____________________________________________________________________________

______________

* ***** ********** ******* ** a biotechnology and academic research

laboratory setting.

--Professional Experience--

UC Davis: Cell Biology & Human Anatomy, Deng Lab Nov 09 to Nov 10

Junior Specialist - Assistant to postdoctoral researcher

(Manuscript in progress, see below)

. Managed mouse colony; responsibilities included genotyping by toe/tail

clip, breeding, weaning, drug administration & daily care of animals.

Assisted with CNS surgical procedures and after-care.

. Skill in brain and spinal cord tissue extraction for glial cell primary

culture or cryo-embeding & sectioning by microtome. Experience with

confocal microscopy and immunohistochemistry.

. DNA and RNA extraction for use in PCR, qRT-PCR and cloning.

. Maintained and organized laboratory supplies and record keeping of waste

disposal for lab.

UC Davis: CNPRC, Abel Lab May 08

- June 09

Staff Research Associate I

. Isolation and primary culture of lymphocytes from primate tissues,

primate biopsy & necropsy.

. Characterization of immune cells by flow cytometry (FACS). Experience

with Flo-Jo computer program.

. Maintenance of equipment and laboratory stock and assistance of GSRs with

their projects.

Antibody Solutions, Inc. Sept

07 - Feb 08

Research Associate (Temporary Assignment)

. Characterization of hybridoma clones in monoclonal antibody production.

. Antibody purification and characterization by ELISA and western blot.

Santa Cruz Biotechnology, Inc.

Nov 05 - Sept 07

Research Associate

. Purification of polyclonal secondary antibodies from animal sera by

affinity chromatography.

. Conjugation of secondary antibodies with HRP and flurochromes,

. Preparation of antigen inoculations for production animals and product

kit assembly.

Research Assistant

. Preparation of animal sera, buffers, media & other solutions.

. Product preparation & kit assembly, record keeping and maintenance of

department stocks.

Laboratory Support

. Organization, sterilization and maintenance of equipment, glassware and

general supplies in support of 5 labs.

Veterinary Centers of America, Hemingway Cat Hospital Sept 02 -

May 04

Technician's Assistant

. Care, feeding, medication and observation of facility-boarded animals.

. Assisted with procedures including gentle restraint of animals, blood

drawing and subcutaneous injections.

--Manuscripts--

V Selvaraj, M Horiuchi, C Guadagna, N Phuong, T Itoh, K Otsu and W Deng

p38? Signaling in Oligodendrocyte Development and Myelination [Manuscript

in preparation]

--Education--

B.S. in Biology University of California, Santa Cruz, CA



Contact this candidate