Training Assistant

Yeon Jean Lee, PhD

PERMANENT ADDRESS Phone: ***-

***-****

**** ******** *** *** * Email:

*******@********.***

Berkeley, CA 94703

PROFILE

Biochemist with 4 years post-doctoral experience in academic setting

. Extensive background in molecular/cell biology and virology

. Background in RNA biology

. Experience with broad-based scientific background; mammalian tissue

culture, immunological assays, DNA/RNA manipulation; protein

expression/purification; qPCR

. Excellent communication, teamwork, and writing skills developed through

academic position; strong analytical skills and attention to detail;

bench and management duties

SKILLS

Northern blot analysis, Taqman real-time qPCR (dye chemistry), PCR,

western blot analysis, in situ hybridization (FISH), mammalian tissue

culture, growth and maintenance of adherent and non-adherent cell lines,

in vitro transcription, reverse transcription, in vitro degradation

assay, immunofluorescence assay (IFA), immunoprecipitation, microscopy

imaging, ELISA, RNA affinity purification, protein purification and

characterization from eukaryotic and prokaryotic systems, FPLC, HPLC,

vector construction, gene cloning, aseptic techniques, experience with

murine model system, mouse genotyping (handling and breeding),

siRNA/shRNA mediated knockdown, EMSA (DNA & RNA), UV crosslinking

experiment, experience with BL-2 virus, induction of lytic cycle in virus-

infected cells, mRNA half-life studies, transient and stable

transfection, transformation, RNA isolation, mutagenesis techniques,

statistics and data analysis, sequence searching on public databases,

sequence alignment, experience in following Standard Operating Procedures

(SOP) and Good Laboratory Practice (GLP)

OBJECTIVE

Obtain industrial research scientist position in molecular biology and

contribute to the technical expertise of an organization invested in the

professional development of its research staff

EDUCATION

Colorado State University, Ph.D., Biochemistry, 12/2006

Colorado State University, M.S., Biochemistry, 08/2002

University of Colorado Health Science Center, Continuing Education,

Immunology, 2000-2001

Colorado State University, B.S., Biochemistry & Microbiology, minor in

Chemistry, 12/1999

RESEARCH EXPERIENCE

UC Berkeley, Dept. of Plant and Microbial Biology

2007 - Current

Postdoctoral Fellow, Laboratory of Dr. Britt Glaunsinger

. Initiated characterization of how viral protein, SOX, promotes host

shutoff through the interception of cellular mRNA degradation pathways

during sarcoma-associated herpesvirus (KSHV) infection

. Determined SOX stimulates cellular transcriptome turnover via a noble

mechanism involving polyadenylation, demonstrated by northern blot

analysis, in situ hybridization (FISH), immunofluorescence

experiments, along with RNAi-mediated knockdown experiments

. Resulted in first author publication in PLoS Biology, and a co-author

publication in Journal of Virology, and a review article

Colorado State University, Dept. of Biochemistry

2001 - 2006

Graduate Research Assistant, Laboratory of Dr. Norman Curthoys

. Characterized how the level of renal glutaminase (GA) is significantly

increased during metabolic acidosis, mediated by a pH-response element

(pHRE) that binds zeta-crystallin/NADPH:quinone reductase (zeta-cryst)

and AUF1

. Independently developed in vitro mRNA decay assay to characterize the

GA turnover mechanism, as well as northern blot analysis, western blot

analysis, and protein purifications including FPLC

. Characterization of pH-responsive induction Phosphoenolpyruvate

carboxykinase (PEPCK), a key regulator of the increased renal

metabolism of glutamine during metabolic acidosis

. Independently developed multiplex real-time PCR to analyze induction

of renal PEPCK, as well as northern blot analysis, mutational analysis

to map and characterize the downstream element that contributes to

induction

Genesis Laboratories, Inc.

2001 - 2001

Chemistry Technician, Richard Poche

. Evaluation of purity and strength for active ingredients, and

cultivation and isolation of Borrelia burgdorferi, a reagent

responsible for the lyme disease, from field samples

. Involved in method development and validation of rodenticides; various

wet and dry chemical techniques were used to test baits for Good

Laboratory Practice (GLP) studies under FIFRA guidelines to compile,

analyze, summarize data that will provide guidance in the development

of a nutria control program

UC Health Science Center, Dept. of Rheumatology

2000 - 2001

Professional Research Assistant, Laboratory of Dr. Michael Holers

. Involved in molecular cloning and generation of monoclonal antibodies

of several complement receptors and membrane regulatory proteins in

the immune response, with emphasis on B lymphocytes and autoimmune

diseases

. Used murine models to study these complement related biologic

processes, analyzed by western, ELISA, and PCR

Colorado State University, Dept. of Microbiology

1999 - 2000

Research Assistant, Laboratory of Dr. Robert Ellis

. The focus of research involved diagnosis, immunoprophylaxis, and

molecular pathogenesis of diseases caused by Clostridium perfringens,

to better the understanding for the vaccine failures and disease

outbreaks

. A multiplex PCR was utilized for detection of the alpha, beta,

epsilon, iota and enterotoxin genes

TRAINING

Biosafety Training, Hazardous Waste Training; Radiation Training; Good

Laboratory Practice Standards Training; Safety Training; Standard

Operating Procedures (SOP) Training; Small Animal Handling Training

CERTIRICATION

Radioactive Materials Usage Certification; Radioactive Materials

Qualified User Certification; GLP (Good Laboratory Practice) Training

Certification; Small Animal Handling Certification

PUBLICATIONS AND FUNDING

Research papers:

How tails define the ending: Divergent roles for polyadenylation in RNA

stability and gene expression. Britt A. Glaunsinger and Yeon J. Lee. RNA

Biology 7:1 1-5; January/February 2010. Review.

Host Shutoff is a Conserved Phenotype of Gamma-Herpesvirus Infection and is

Orchestrated Exclusively from the Cytoplasm. Sergio Covarrubias1, Justin

M. Richner2, Karen Clyde2, Yeon J. Lee2, and Britt A. Glaunsinger2

1Division of Infectious Diseases and Immunity, School of Public Health.

2Department of Plant and Microbial Biology, University of California

Berkeley, Berkeley, CA 94720-3102. J. Virol. 2009 : JVI.01051-09v1.

Faculty of 1000 Biology - Evaluated as "Must-read"

Aberrant Herpesvirus-Induced Polyadenylation Correlates with Cellular

Messenger RNA Destruction. Yeon J. Lee and Britt Glaunsinger. Department of

Plant and Microbial Biology. University of California Berkeley, Berkeley,

CA 94720-3102. PLoS Biol. 2009 May 5;7(5):e1000107.

Highlighted as a "Editor's Choice" in Science and Nature Reviews

Genetics

Faculty of 1000 Biology - Evaluated as "Must-read"

Renal Response to Metabolic Acidosis: Role of mRNA Stabilization. Ibrahim,

H*., Lee, YJ*, Curthoys, NP. Kidney Int. 2008 Jan;73(1):11-8. Epub 2007 Oct

3. Review. (* Co-first author)

Research grant:

. Individual, postdoctoral, 07/2010-06/2013, National Cancer Institutes,

GRANT10414549

. Individual, predoctoral, 01/2003-03/2006, National Institutes of Health

Minority Supplement to Grant DK37124

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