Assistant Medical

James Gaudette

**** ******* ******* ****, ***. #*503 San Diego, Ca. 92108 917-***-**** ***********@*****.***

JOB OBJECTIVE

Seeking research assistant position specializing in protein, cell, and molecular biology

techniques and applications

QUALIFICATION HIGHLIGHTS

• 5 years of independent laboratory research experience

• Proficient at recording findings and interpreting results

• Graduate level course work in immunology and infectious disease

• Highly motivated to learn and contribute to the field of science

EDUCATION

Albert Einstein College of Medicine, Bronx, NY

• Master of Science (2007 2011)

Worcester Polytechnic Institute, Worcester, MA

• Bachelor of Science in Molecular Genetics (2003 2007)

RESEARCH EXPERIENCE

Albert Einstein College of Medicine, Bronx, NY

Dr. Steve Almo (June 2010 – Present)

• Structural characterization of members of the C type lectin superfamily

• Assembled a cDNA library of 69 lectins that do not have solved structures

• Domain optimized 43 lectins for efficient expression and crystallization

• Expressed and purified lectins in both eukaryotic and prokaryotic systems

• Identified conditions for the crystallization of a C type lectin

Dr. Vinayaka Prasad (May 2009 – May 2010)

• Investigated the molecular basis for subtype specific differences in patients with

HIV associated dementia

• Monitored the phenotypic changes in macrophages infected with wild type or

mutated strains of HIV

University of Massachusetts Medical School, Worcester, MA

Dr. Mario Stevenson (June 2006 – June 2007)

• Worked to identify novel restriction factors of HIV replication

• Utilized RNAi to knock down HIV interacting host cell proteins

• Over expressed HIV interacting proteins in infected cells

RELEVANT SKILLS

High Throughput Techniques

• AKTA Express, Unicorn, Cloning, E. coli Transformation, autoinduction

Recombinant Protein Production, Molecular Biology, and Cell Culture

• Insect cell, mammalian, and E. coli protein expression

• Affinity, SEC, and IEX chromatography

• Western Blot, SDS PAGE, FPLC

• PCR, qPCR, RT PCR, RNA isolation, molecular cloning, vector creation

• Primary cell culture, transformed cell lines, transfection, infection, FACS, RNAi

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