Ms Office Computer

Arya M. Koshti

***** ***** **, *******, **-***** . Mobile 510-***-**** .

abgz63@r.postjobfree.com

Objective

To obtain Research Associate position within your organization where my

skills in molecular biology techniques can be an asset towards achieving

the goal of the company.

Summary of QUalification

. M.S. in molecular biology with 3 years hands on experience in biotech

industry and academic institution.

. Experience with basic Molecular techniques, mammalian cell culture

techniques, viral infection and titration.

. Detailed oriented, have keen interest in keeping up with the current

scientific literature and good at following the detailed protocols.

. Maintained accurate and detailed laboratory records with experimental

procedures, results and in depth data analysis.

. Proficient in general computer skills (MS Excel).

. Can work with minimal supervision, under deadlines, great team spirit and

good communication and presentation skills (oral and written).

PROFESSIONAL EXPERIENCE

Genentech, Inc., San Francisco, CA

July 2010-

December 2010

Intern, Microbial Pathogenesis Department (HCV)

Key contributor to the successful completion of multiple projects.

. Generated SEAP reporter cell line by stable transfection of Huh-7.5 cells

followed by selection with puromycin to study HCV replication and

infectivity.

. Made HCVpp (viral) Stocks for routine lab experiments.

. Expressed cell surface receptors for in-vitro studies of HCV entry in

different cell lines.

California State University, East Bay, Hayward, CA

September 2009-May2010

Teaching Associate- Biology 1401 and 1402

Educated and evaluated undergraduates (25 students per class) during labs

on molecular and plant biology concepts.

laboratory Skills

Virology Lab Experience: Cell Culture (NIH/3T3, Huh-7.5, AML-12, 293T),

Transduction using retrovirus/lentivirus, Generation of Viral Stocks and

Infection, Neutralization Assay to Screen for Antiviral Compounds,

Determining Viral Titers, TCID50, Luciferase Assay, Staining for viral

core proteins, FACS, Fluorescent microscopy, In-vitro transcription,

Formaldehyde(RNA) gels.

Molecular Lab Experience: DNA/RNA isolation, PCR, Sequencing, qRT-PCR,

Ligation mediated PCR, Inverse PCR, TA Cloning, Primer Designing, Nanodrop,

Restriction Digestion, Gel Electrophoresis, Gateway Cloning, Southern

Blotting, Site Directed Mutagenesis.

Cell Culture Expereince: MTS assay, Cryopreservation, Established primary

cell culture, Maintained and sub-cultured Primary cell line, Stable and

transient transfection, Cloning of established cell line (limited dilution

method).

Protein Experience: Size exclusion/Ion-exchange Chromatography, Western

Blotting, SDS PAGE, Protein extraction Expressed proteins using expression

vectors,2D-Gels, Determined specific activity of the enzyme, Bradford

Assay, Prepared buffers.

BASIC BIOINFORMATICS TOOLS and COMPUTER PROFICIENCY

. Various Web-based applications for Bioinformatics Analysis (PDB, Pubmed,

and comparison using BLAST, Clustal W,PFAM, BLOCKS), Sequencer, Chromas,

Plasmid Editor, SAS Programming.

. Proficient in MS Office (Word, Excel).

EDUCATION

Masters in Science (Biology), California State University, East Bay,

Hayward, CA, GPA 3.98

Master Thesis: Cloning of an Integrase gene from the LTR-Retroelements in

the Liliaceae family.

. Did qRT-PCR for determining the expression of Integrase gene.

. Cloned and sequenced Integrase gene from LTR-retrotransposons.

. Developed Assay for determining Integrase (enzyme) Activity.

Relevant Course Work: Cell and Molecular Biology, Functional Genomics, PCR,

Sequencing and Fragment Analysis, Protein Chemistry, Cell Culture, Advance

Molecular Techniques, Bioinformatics, Microbiology, Genetics, Biochemistry

Bachelors in Science (Microbiology), Nagpur University, Nagpur, India

(Overall Grade A).

Certificate, Computer Application in Biotechnology (Bioinformatics), Ohlone

College, Fremont, CA, GPA 4.0

Communication Skills

. Meticulous and sharp, with strong skills in data interpretation and

analysis, presentations, and report writing.

. A talented communicator who articulates with precision to all levels of

professionals.

REFERENCES

To be provided upon request.

. Authorized to work in USA.

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