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CURRICULUM VITAE

Evgeniy B. Eruslanov

Research Scientist,

College of Medicine, Department of Urology

University of Florida, Gainesville, FL

Mailing address: 7960 SW 84th way, Gainesville, FL,

32608, Phone: 352-***-****

Email: *.*********@*******.***.***.

PROFESSIONAL SKILLS

Cell culturing: short-term proliferative assays (antigen-specific, CD3/CD28); T cell cloning;

culturing tumor cell lines; preparation and separation of lung and spleen T cells, interstitial

lung macrophages; MLR (mixed lymphocyte reaction); isolation of tumor associated myeloid

cells from mouse and tumor tissue; preparation of bone-marrow derived macrophages;

dendritic cells and human monocyte-derived DC; purification of peritoneal neutrophils and

macrophages; separation and enrichment of different cell populations using specific antibody

labeled with magnetic beads (MACS cell separation); chemotaxis assay

Flow cytometry (10 years of experience): four-color staining of the cell surface markers

using fluorescent Abs, analysis of cell proliferation using BrdU incorporation assay or

labeling of cell with CSFE dye, analysis of cell cycle using BrdU and 7-AAD DNA staining,

detection of cytokines production by CD4, CD8 T cells, macrophages and dendritic cells

using an intracellular cytokine staining, detection of cell apoptosis and necrosis; evaluation of

phagocytic activity of myeloid cells using fluorescent dextran or fluorescent bacteria;

detection of ROS production by using oxidized DCFDA; detection of NO with DAF-FM;

detection of Ca2+ influx with Fluo Calcium indicators; BDTM Phosflow (Protein

Phosphorylation Analysis), data analysis using Beckman Coulter’s CXP software (former

Expo32), Becton Dickinson’s CellQuest software, FlowJo software.

Cytokine Multi-plex assay (Luminex); ELISA; ELISpot; Meso Scale Discovery Assay

Immunohistochemistry and Immunofluorescence staining of frozen and paraffin-embedded

tissue sections, Immunofluorescence microscopy

Evaluation of functional activity of myeloid cells (macrophages, dendritic cells and

neutrophils);

Evgeniy B Eruslanov, 2

DNA and RNA isolation and purification, real-time qPCR; Adenoviral or lentyviral-mediated

gene expression

2 years Bio Safety Level-3 experience

Experiments in vivo:

Radiation bone-marrow chimera approach; Adoptive transfer of bone-marrow cells; The

experimental surgical procedure to allow intratracheal injection; Analysis of cells division

and proliferation in vivo using CSFE labeled cells, BrdU incorporation; Mouse tumor models;

PROFESSIONAL EXPERIENCE

2008-present University of Florida Gainesville, FL

Research Scientist, College of Medicine, Department of Urology

Studying the role of CCL1/CCR8 chemokine axis in the tumor

microenvironment

Investigating the circulating and tumor infiltrating myeloid cell subsets

(granulocyte-type CD15high CD33low cells and monocyte-type CD15low

CD33high cells) in cancer patients.

2006-2008 University of Florida Gainesville, FL

Postdoctoral Research Associate, College of Medicine, Department of Urology

Investigated the prostaglandin E2 (PGE2) and 15-hydroxyprostaglandin

dehydrogenase (15-PGDH) in the regulation of local anti-tumor immune

response

Characterized the role of tumor induced VEGFR1/CD11b myeloid cells.

2004- 2006 Harvard School of Public Health, Harvard University Boston, MA

Postdoctoral Research Fellow, Laboratory for TB, Department of Immunology and

Infectious Diseases

Investigated the of innate and acquired immune responses in infected mice

with distinct susceptibility to tuberculosis infection

Characterized the Ipr1 protein function in macrophages, the protein of a novel

gene Ipr1 (intracellular pathogen resistance 1)

Developed the inducible Ipr1gene expression systems in macrophages, using

lentyvirus constructs

Bio Safety Level-3 experience

Evgeniy B Eruslanov, 3

2002-2004 Central Institute for Tuberculosis Moscow, Russia

PostDoc Fellow, Laboratory for Immunogenetics,

Characterized local mechanisms of immune response to TB infection in the

lung of infected mice with opposite susceptibility to infection;

Investigated the M.tuberculosis interaction with lung macrophages and

neutrophils isolated from susceptible and resistant mice to TB infection;

Investigated the neutrophils response to TB infection in susceptible and

resistant mice.

EDUCATION AND ACADEMIC TRAINING

2002 Central Institute for Tuberculosis

Gamaleya’s Institute of Epidemiology and Microbiology Moscow, Russia

Laboratory for Immunogenetics

Ph.D. Immunology

Pirogov’s Russian State Medical University

1999 Moscow, Russia

Medico-Biological Department

M.S. Immunology, (summa cum laude), Department of Immunology

PEER –REVIEWED PUBLICATIONS:

1. Eruslanov E, Neuberger M, Daurkin I, Rosser C,Perrin G, Algood C, Dahm P, Vieweg J,

Gilbert S and Kusmartsev S. Circulating and tumor-infiltrating myeloid cell subsets in

patients with bladder cancer, International Journal of Cancer, 2011, in press

2. Eruslanov E, Daurkin I, Vieweg J, Daaka Y, and Kusmartsev S. Aberrant PGE2

metabolism in bladder tumor microenvironment promotes immunosuppressive phenotype

of tumor-infiltrating myeloid cells. International Immunopharmacology, 2011, Feb 9.

(Epub ahead of print)

3. Eruslanov E, Daurkin I, Ortiz J, Vieweg J, and Kusmartsev S. Pivotal Advance: Tumor-

mediated induction myeloid-derived suppressor cells and M2-polarized macrophage by

altering intracellular PGE2 metabolism in myeloid cells. Journal of Leukocyte Biology,

2010, 88(5):839-48

Evgeniy B Eruslanov, 4

4. Daurkin I, Eruslanov E, Vieweg J, Kusmartsev S. Generation of antigen -presenting cells

from tumor-infiltrated CD11b myeloid cells with DNA demethylating agent 5 -aza-2'-

deoxycytidine. Cancer Immunol. Immunother. 2010, 59(5):697-706

5. Glushakova LG, Lisankie MJ, Eruslanov EB, Ojano-Dirain C, Zolotukhin I, Liu C,

Srivastava A, Stacpoole PW. AAV3-mediated transfer and expression of the pyruvate

dehydrogenase E1 alpha subunit gene causes metabolic remodeling and apoptosis of

human liver cancer cells. Mol. Genet. Metab. 2009, Nov, 98(3): 289-99

6. Sakamoto N, Porvasnik S, Kusmartsev S, Eruslanov E, Cao W, Urbanek C, Rosser CJ.

Effects of CXCR4 antagonist CTCE-9908 on prostate tumor growth. Prostate. 2009, Jul

8; 69(13):1460-1469

7. Eruslanov E, Kaliberov S, Daurkina I, Kaliberova L, Buchsbaum D, Vieweg J,

Kusmartsev S. Altered expression of 15-prostaglandin dehydrogenase in intra-tumoral

CD11b myeloid cells: a new mechanism of immune evasion in cancer,. J. Immunology.

2009. Jun15; 182(12): 7548-57

8. Kusmartsev S, Su Z, Heiser A, Dannull J, Eruslanov E, Kübler H, Yancey D, Dahm P.

and Vieweg J. Reversal of myeloid cell-mediated immunosuppression in patients with

metastatic renal cell carcinoma. Clinical Cancer Research. 2008. Dec 15:14(24):8270-

8278

9. Sakai Y., Goodison S., Kusmartsev S., Fletcher B., Eruslanov E., Cao W., Porvasnik S.,

Namiki K, Anai S., Rosser CJ. Bcl-2 mediated modulation of vascularization in prostate

cancer xenografts. Prostate. 2009, Apr 1: 69(5):459-70.

10. S. Kusmartsev, E. Eruslanov, H. Kübler, T. Tseng, Y. Sakai, Z. Su, S. Kaliberov, C.

Rosser, P. Dahm, D. Siemann and Johannes Vieweg . Oxidative Stress Regulates

Expression of VEGFR1 in Myeloid Cells: Link to Tumor-Induced Immune Suppression

in Renal Cell Carcinoma. J. Immunology. 2008. Jul 1; 181(1):346-53

11. Yan BS, Pichugin AV, Jobe O, Helming L, Eruslanov EB, Gutierrez-Pabello JA, Rojas

M, Shebzukhov YV, Kobzik L, and Kramnik I. Progression of Pulmonary Tuberculosis

and Efficiency of Bacillus Calmette-Guerin Vaccination are Genetically Controlled via a

Common sst1-Mediated Mechanism of Innate Immunity. J. Immunology., 2007, Nov 15;

179(10):6919-32

12. Pan H, Mostoslavky G, Eruslanov E, Kotton DN, Kramnik I. Dual-promoter lentiviral

system allows inducible expression of noxious proteins in macrophages. J Immunological

Methods. 2008, Jan 1; 329 (1-2):31-34

Evgeniy B Eruslanov, 5

13. Orlova MO, Majorov KB, Laydova IV, Eruslanov EB, M’lan CE, Greenwood CM,

Shurr E and Apt AS. Constitutive differences in gene expression profiles parallel genetic

patterns of susceptibility to tuberculosis in mice. Infection and Immunity. 2006 Jun;

74(6):3668-72.

14. K. B Majorov, E. B. Eruslanov, E.I. Rubakova, T. K. Kondratieva, and A. S. Apt.

Analysis of cellular phenotypes that mediate genetic resistance to tuberculosis using a

radiation bone marrow chimera approach. Infection and Immunity. 2005, Sep,

73(9):6174-8.

15. E. B. Eruslanov, I. V. Lyadova, T. K. Kondratieva, K. B Majorov, I.V Scheglov, M. O.

Orlova, and A. S. Apt . Neutrophil responses to Mycobacterium tuberculosis infection in

genetically susceptible and resistant mice. Infection and Immunity. 2005, Mar; 73(3):

1744-53

16. Mischenko VV, Kapina MA, Eruslanov EB, Kondratieva EV, Lyadova IV, Young DB,

Apt AS. Mycobacterial dissemination and cellular responses after 1-lobe restricted

tuberculosis infection of genetically susceptible and resistant mice. J Infect Dis. 2004

Dec 15; 190 (12):2137-45.

17. E. B. Eruslanov, K. B Majorov, M. O. Orlova, V. V. Mischenko, T. K. Kondratieva, A.

S. Apt and I. V. Lyadova. Lung cell responses to M. tuberculosis in genetically

susceptible and resistant mice following intratracheal challenge. Clin. Exp. Immunol.,

2004, 135:19-28.

18. K. B. Majorov, I. V. Lyadova, T. K. Kondratieva, E. B. Eruslanov, E. I. Rubakova, M.

O. Orlova, V. V. Mischenko, and A. S. Apt. Different innate ability of I/St and A/Sn

mice to combat virulent M. tuberculosis: phenotypes expressed in lung and extra-

pulmonary macrophages. Infect. Immunity 2003, 71: 697-707.

19. V. Lyadova, H. M. Vordermeier, E. B. Eruslanov, S. V. Khaidukov, A. S. Apt, R. G.

Hewinson. Intranasal BCG vaccination protects BALB/c mice against virulent

Mycobacterium bovis and accelerates production of IFN-γ in their lungs. Clin. Exp.

Immunol., 2001, 126:274-279.

20. Lyadova I. V., E. B. Eruslanov, V. V. Yeremeev, K. B. Majorov, B. V. Nikonenko, A.

V. Pichugin, S. V. Khaidukov, T. K. Kondratieva, A. S. Apt. Comparative analysis of T

lymphocytes recovered from the lungs of mice genetically susceptible, resistant and

hyperresistant to Mycobacterium tuberculosis-triggered disease. J. Immunology., 2000,

165:5921-5931.

Evgeniy B Eruslanov, 6

BOOKS

E. Eruslanov and S. Kusmartsev., chapter “Identification of ROS using oxidized DCFDA

and flow-cytometry”, Methods in Molecular Biology, volume “Advanced Protocol in

Oxidative Stress II”, D.D. Armstrong (ed.), vol. 594, 2010

RECENT PRESENTATIONS

AACR 101st Annual Meeting 2010 in Washington, DC, poster session, April, 2010

The College of Medicine 2010 Celebration of Research at University of Florida, poster #12

session, Gainesville, Florida, April, 2010

An AACR 100th Annual Meeting in Denver, CO, poster session, April 21,2009

Fifth Biennial Meeting, Moffitt Cancer Center, “Regulatory Myeloid Suppressor Cells in

Health & Disease”, Clearwater Beach, Florida, March 12-15, 2009

Research Day at University of Florida, Gainesville, Florida, poster session, April, 2009

An AACR Special Conference in Cancer Research “Tumor Immunology: New

Perspectives”, oral presentation, Miami, Florida, December2-5, 2008

11th

Annual Joint Cancer Conference of the Florida Universities, AMGEN Young

Investigators Forum, oral presentation, Palm Beach, FL, September 13, 2007

American Association for Cancer Research. Annual Meeting April, Los Angeles, CA, 14-

18, 2007, Abstract # 2552.

SCIENTIFIC AWARDS

1999 Laureate of 4th Pirogov's scientific conference, Moscow, Russia

1999 University of Rostock travel grant, Hanseatic Students' Days of science, Rostock,

Germany

1999 Winner of the best diploma award” of Medico-Biological Department, Russian State

Medical University, Moscow, Russia

2001 Instituto Italiano per gli Studi Filosofici travel grant award, Scuola Superiora

d'Immunologia Ruggero Ceppellini, Naples, Italy

2002 Instituto Italiano per gli Studi Filosofici travel grant award, Scuola Superiora

d'Immunologia Ruggero Ceppellini, Naples, Italy

2007 AMGEN fellows travel grant award, Cancer Conference of the Florida Universities, AMGEN

Young Investigators Forum,

Evgeniy B Eruslanov, 7

2008 AFLAC, Inc. Scholar-in-Training Award, AACR Conference “Tumor Immunology: New

Perspectives”

PROFESSIONAL ASSOCIATION MEMBERSHIPS

1. American Association for Cancer Research (AACR), member #147323.

2. American Association of Immunologists ( AAI ) member # 00213207

PATENTS

S. Kusmartsev, S. Kaliberov, E. Eruslanov and J. Vieweg., TUMOR GROWTH

INHIBITION VIA CONDITIONING OF TUMOR MICROENVIRONMENT., Serial No.

61/041,106;

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