Devendra Hiraman Dusane
Institutional Address Permanent Address
Institute of Bioinformatics and Biotechnology (IBB) 10, Shramnagar colony
University of Pune, Pune: 411 007 Upnagar, Nasik: 422 006
Maharashtra, India Maharashtra, India
e-mail: ****************@*****.**.**; ********@*******.**.**
Website: www.epernicus.com/dhd
Mobile: 009*********** ; 009***********
I. Educational Qualification
PhD (Biotechnology): Thesis submitted.
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Master’s of Science in Microbiology, University of Pune (1st Division): 1999 – 2001.
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Bachelor’s of Science, University of Pune (1st Division): 1996 – 1999.
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II. Research Interest
Biofilms; Quorum Sensing; Infectious Diseases; Natural products; Marine Ecology;
Nanotechnology; Biofuels
III. Awards & Honors
John David Williams Memorial award (International Society of
1.
Chemotherapy, UK) for excellence in the study of Antimicrobial agents (2011).
Selected for the Novartis India BioCamp 2011.
2.
Council of Scientific and Industrial Research (CSIR, India) travel grant (2011).
3.
Board of College and University Development (BCUD), University of
4.
Pune, grant for the 21st ECCMID-27th ICC 2011 at Italy (7-11, May) (2011).
Department of Science and Technology (DST) and Department of
5.
Biotechnology (DBT, India) travel grant (2011).
European Society of Clinical Microbiology and Infectious Diseases
6.
(ESCMID) attendance grant for course work “Biofilms in nosocomial fungal
Infections” Institute Pasteur, Paris, France (2011).
Biography appeared in Marquis Who’s Who of the World (2010).
7.
University Grants Commission (UGC) travel grant for “II-International
8.
Conference on Environmental, Industrial and Applied Microbiology”
Seville, Spain conference during Nov 28th - Dec 1st (2007).
Research fellowship awarded by Bhabha Atomic Research Centre
9.
(BARC), Department of Atomic Energy (DAE), Government of India under
(BARC–UoP programme) (2005).
IV. Positions held
2007 – 2010 : Senior Research Fellow (Dept. of Atomic Energy), BARC–UoP
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2005 – 2007 : Junior Research Fellow (Dept. of Atomic Energy), BARC–UoP
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2004 – 2005 : Research Associate, SRISTI, Ahmedabad, India.
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2001 – 2004 : Microbiologist, PRAJ Industries limited, Pune, India.
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V. Professional Activities
Life member of Association of Microbiologists of India (AMI) since 2008.
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Manuscript reviewing member for peer reviewed journals: Journal of Bacteriology
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Research (JBR); Journal of Cell and Animal Biology (JCAB)
VI. Doctoral Topic
“Antimicrobial compounds from marine microbes: Search for potential biofilm
disruptors”
• Advisor: Dr. Smita Zinjarde (Associate Professor, IBB, University of Pune, India)
• Co–Advisor: Professor BA Chopade (Director, IBB, University of Pune, India)
VII. Research Techniques
• Biofilm formation (single/mixed biofilms) and disruption techniques.
• Quorum Sensing bioassays (QS and QS Inhibition).
• Confocal laser scanning microscopy, Fluorescence and scanning electron
microscopy.
• Cell culture, cytotoxicity.
• Microtiter plate assays, Multichannel flow-cells.
• Antimicrobial, biosurfactant and emulsification assays.
• Protein purification: Cell Prep, LC-MS, Column chromatography, 2D gel.
• FTIR, NMR, FPLC, HPLC, MALDI, GC-MS.
• Molecular Techniques: PCR, RT-PCR, FISH, Gel electrophoresis, DNA isolation.
• Imaging softwares: COMSTAT, IMARIS and ImageJ.
• Fermentation techniques, Reactor scale up, process optimization, QC.
VIII. Professional Experience
1. Department of Atomic Energy (DAE); BARC-UoP Fellowship
From November 2005–October 2010 on a collaborative research Project entitled
“Antibacterial compounds from marine epizoic and epiphytic bacteria: Search for
potential biofilm disruptors”
• Principal Investigator: Dr. Smita Zinjarde (Associate Professor, Pune University)
• Co-Principal Investigator: Dr. V. P. Venugopalan (Head, BBPS, Kalpakkam, India)
2. SRISTI, Ahmedabad, Gujarat, India (www.sristi.org; www.nifindia.org)
As a Research Associate (RA) from November 2004 – November 2005.
Intellectual Property Rights (IPR)
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Development of antimicrobial herbal formulations.
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Study of soil micro flora and its effect on crop development.
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Isolation of antibiotic producing Actinomycetes.
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Validation, value addition and documentation of the traditional practices.
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Development of Medicinal Plant Database: NISSAT in four different local languages.
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Interaction with Scientists, entrepreneurs, traditional knowledge holders for
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dissemination of scientific knowledge within the society.
3. Praj Industries Ltd. Pune, Maharashtra, India (www.praj.net)
As a Microbiologist from November 2001 – November 2004.
Praj is a company with expertise in biotechnology, bioprocess, design process and
Engineering in the field of Biofuels with project worldwide.
• Yeast culture collection, screening of productive strains.
• Process optimization and scale up, Enzyme formulation.
• QC check, documentation, Process development, troubleshooting industrial
fermentation.
• Customer care, handling industrial fermentation related problems.
• Handled more than 25 project sites.
IX. LIST OF PUBLICATIONS: 07
Reviews: 01
1. Dusane, D.H., S.S. Zinjarde, V.P. Venugopalan, R.J.C. McLean, M. Weber and K.S.M.
Rahman. (2010). Quorum sensing: Implications on rhamnolipid biosurfactant
production. Biotechnology and Genetic Engineering Reviews. 27:159-184.
Research Papers: 06
2. Dusane et al. (2011). Antibiofilm potential of a glycolipid biosurfactant produced by a
tropical marine strain of Serratia marcescens. Biofouling. 27(6):645-654. (I.F. 4.415).
3. Kazemi Pour, Dusane et al. (2011). Biofilm formation by Acinetobacter baumanii
strains isolated from urinary tract infection and urinary catheters. FEMS Immunology
Medical Microbiology. 62(3):328-338. (I.F. 2.494).
4. Dusane, D. H., P. Matkar, V. P. Venugopalan, A.R. Kumar and S. S. Zinjarde. (2011).
Cross species induction of antimicrobial compounds, biosurfactants and quorum sensing
inhibitors in tropical marine epibiotic bacteria by pathogens and biofouling
microorganisms. Current Microbiology. 62(3):974-80. (I.F. 1.51).
5. Dusane, D.H., Y.V. Nancharaiah, V.P. Venugopalan and S.S. Zinjarde (2010).
Rhamnolipid mediated disruption of marine Bacillus pumilus biofilms. Colloids and
Surfaces B: Biointerfaces. 81: 242-248. (I.F. 2.780).
6. Dusane, D.H., J.K. Rajput, A.R. Kumar, Y.V. Nancharaiah, V.P. Venugopalan and S.S.
Zinjarde (2008). Disruption of fungal and bacterial biofilms by lauroyl glucose. Letters
in Applied Microbiology. 47: 374-379. (Most downloaded article in 2008 as per
Microbiologist Magazine, March 2009, Vol. 10, No. 1). (I.F. 1.647).
7. Dusane, D.H., Y.V. Nancharaiah, V.P. Venugopalan, A.R. Kumar and S.S. Zinjarde
(2008). Biofilm formation by a biotechnologically important tropical marine yeast
isolate, Yarrowia lipolytica NCIM 3589. Water Science and Technology. 58(6): 1221-
1229. (I.F. 1.056).
Manuscripts in Preparation: 03
1. Dusane et al. Disruption of fungal and bacterial biofilms by a novel protein from
marine Bacillus licheniformis. PLOS One.
2. Dusane et al. Disruption of Yarrowia lipolytica biofilms by rhamnolipid
biosurfactant. Colloids and Surfaces B: Biointerfaces.
3. Dusane et al. Silver nanoparticles disrupts detrimental Candida albicans and
Pseudomonas aeruginosa biofilms. Colloids and Surfaces B: Biointerfaces.
Popular Articles: 03
1. An article on science education system in India & initiatives for improvement
http://ezinearticles.com/?Science-Education-In-India&id=836401
2. An article on managing wild yeast contamination in industrial fermentation process
http://www.cheresources.com/wild_yeast_contamination.shtml
3. An informative article on biofilms http://ezinearticles.com/?Biofilms---A-Prelude-of-
Organized-Microbial-Community&id=861815
X. Conferences attended (Selected)
1. Dusane, D. H., S. Dam, R. Nair, A. R. Kumar, A. Beauvais and S. S. Zinjarde.
Antibiofilm and anticancer activity of farnesol: a quorum sensing molecule. Biofilms:
Friend or Foe? Workshop at Berlin, Germany 22-23, June 2011. (Poster presentation)
2. Dusane, D. H., V. Pawar, Y. V. Nancharaiah, V. P. Venugopalan, A. R. Kumar and S.
S. Zinjarde. A novel glycolipid from marine Serratia marcescens disrupts detrimental
Candida albicans BH and Pseudomonas aeruginosa PAO1 biofilms. 21st ECCMID/27th
ICC, 7-10th May 2011, Milan Italy. (Poster presentation)
3. Dusane, D.H., S.R. Damare, Y.V. Nancharaiah, N. Ramaiah, A.R. Kumar, V.P.
Venugopalan and S.S. Zinjarde. Disruption of fungal and bacterial biofilms by a novel
antimicrobial protein from Bacillus licheniformis D1. International conference on
“Biofilms in nosocomial fungal infections”, January 31st – 1st February 2011, Pasteur
Institute, Paris, France. (Oral Communication)
4. Dusane, D.H., S. Dam, Y. V. Nancharaiah, V.P. Venugopalan, A.R. Kumar and S.S.
Zinjarde. Disruption of Yarrowia lipolytica biofilms by rhamnolipid biosurfactant. 51st
annual conference of Association of Microbiologists of India on “International
symposium on recent advances in cross-disciplinary microbiology: Avenues and
challenges & International workshop on rRNA sequencing, phylogeny and next
generation genome sequencing, December 14-17, 2010, Birla Institute of Technology,
Mesra, Ranchi, India. (Poster presentation)
5. Pawar, V.S., D.H. Dusane, A.R. Kumar and S.S. Zinjarde. Disruption of detrimental
biofilms by bioactive compounds from a marine strain of Serratia marcescens, 49th
annual conference of Association of Microbiologists of India, November 14 -16, 2008.
University of Delhi, India. (Poster presentation)
6. Matkar, P., D.H. Dusane, A.R. Kumar, V.P. Venugopalan and S.S. Zinjarde. Biofilm
disruption by marine epibionts isolated from Perna viridis, 49th annual conference of
Association of Microbiologists of India, November 14 -16, 2008. University of Delhi,
India. (Poster presentation)
7. Dusane, D.H., Y.V. Nancharaiah, V.P. Venugopalan, A.R. Kumar and S.S. Zinjarde.
Biofilm formation by a biotechnologically important yeast: Yarrowia lipolytica NCIM
3589, IWA Biofilm Technologies 2008, January 8-10, 2008. Singapore. (Poster
presentation)
8. Dusane, D.H., Rajesh Kumar, Y.V. Nancharaiah, V.P. Venugopalan and S.S. Zinjarde.
Antibacterial compounds from marine epizoic and epiphytic bacteria: search for
potential biofilm disruptors, International Symposium on New Frontiers in Marine
Natural Product Research (NFMNPR), February 23-24, 2007. National Institute of
Oceanography, Goa, India. (Poster presentation)
9. Pakbaz, F., A. Krishnan, D.H. Dusane, A.R. Kumar and S.S. Zinjarde. Degradation of
1-bromodecane by a novel Pseudomonas sp., Microbes: Biofactories of the future, 48th
annual conference of Association of Microbiologists of India, December 18-20, 2007.
IIT-Madras, India. (Poster presentation)
10. Rajput, J.K., D.H. Dusane, A.R. Kumar, V.P. Venugopalan and S.S. Zinjarde,
Disruption of biofilms using Lauroyl glucose ester, International conference on Recent
Advances in Marine Antifouling Technology (RAMAT- 2006), November 6-8, 2006.
NIOT, Chennai, India. (Oral Communication)
XI. Personal Information
Date of Birth : March 28, 1979
Marital Status : Married
XII. References
Dr. Smita Zinjarde Professor RJC McLean
Associate Professor, Department of Biology,
Institute Bioinformatics Biotechnology, Texas State University- San Marcos,
University of Pune, Ganeshkhind, 601, University Drive,
Pune- 411007, India. San Marcos, TX 78666, USA.
e-mail: *****@*******.**.** e-mail: ******@*******.***
Dr. V.P. Venugopalan
Head,
Biofouling & Biofilm Process Section,
Water & Steam Chemistry Division,
IGCAR, BARC (F) Kalpakkam, India.
e-mail: ***@*****.***.**